EB病毒感染患儿免疫细胞功能变化及其临床意义

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Abstract Objective To study the changes of lymphocyte subsets and T lymphocyte function in peripheral blood caused by Epstein-Barr virus (EBV) infection in children, and to explore the relationship between EBV DNA load and lymphocyte subsets. Methods Twenty-five children with EBV infection were selected as the infection group from January 2015 to July 2018 in the Aerospace Center Hospital (“our hospital” for short), and another 25 healthy subjects who underwent physical examination in our hospital in the same time were selected as the control group. Flow cytometry was used to detect the expression of perforin and granulase B in peripheral blood lymphocyte subsets and CD8+ T lymphocyte. The levels of IFN-γ and interleukin 4 (IL-4) secreted by peripheral blood mononuclear cells (PBMC) were measured by enzyme-linked immunosorbent assay (ELISA). Serum EBV DNA load was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). Results The ratios of CD3+ and CD8+ cells in peripheral blood of the infected group were significantly higher than that of the control group (P < 0.001). The ratio of CD4+ cells was significantly lower than that of the control group (P < 0.001), but there was no significant difference between the absolute values of the two groups(P > 0.05), the ratio of CD4/CD8 in the infection grous was significantly lower than the control group (P < 0.001), but there was no linear correlation between the change in the proportion and serum EBV DNA load (r = 0.19, P > 0.05). The ratio and absolute value of CD19+ cells were lower than those of the control group (P < 0.001). There was no significant difference in the ratio and absolute value of CD16+CD56+ NK cells between the two groups (P > 0.05). Compared with the control group, the secretion of IFN-γ was increased in the infection group (P < 0.01), and the secretion of IL-4 was decreased (P < 0.05). The secretion of perforin and granzyme B in CD8+ T cells were significantly increased (P < 0.05). Conclusion The lymphocyte subsets and their functions in the peripheral blood of the infected group have obvious changes. It is important to detect the changes of lymphocyte subsets for understanding the cellular immune function of children and guiding clinical treatment.

Key words： Epstein-Barr virus Lymphocyte subsets Interferon gamma Interleukin 4 Perforin

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	LIU Xuekai1 WANG Yingshi1 XIN Qin1 GAO Yuhan2

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LIU Xuekai1 WANG Yingshi1 XIN Qin1 GAO Yuhan2. Changes of immune cell function in children with EB virus infection and its clinical significance[J]. 中国医药导报, 2019, 16(8): 72-75,95.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I8/72

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