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2017 Vol. 14, No. 22
Published: 2017-08-05
 
 
4 Effect of ACAT1 gene knockdown on the biological behavior of the prostate cancer cells
CHEN Xin1 XU Ximing1 GAN Yuanyuan1 CAO Dedong1 WU Long1 CHEN Zhiyuan2
Objective To observe the effect of ACAT1 on human prostate cancer cells proliferation, invasion and migration. Methods Human prostate cancer cells (PC-3 cells) were cultured, then the expression of ACAT1 gene in PC-3 cells were interfered using Hiperfect transfection reagent, the expression of ACAT1 gene before and after transfection was detected by Real-time PCR. The proliferation rates of PC-3 cells in the blank control group (serum-free 1640 medium added), the negative control group (negative-siRNA-HiperFect complex added) and the ACAT1 group (ACAT1 siRNA-HiperFect complex added) were analyzed by CFSE staining. The changes of cell proliferation before and after ACAT1 gene silencing were compared. Transwell assays were used to evaluate the effect of ACAT1 gene interfering on cells migration and invasion. Results Compared with the blank control group and the negative control group respectively, ACAT1 mRNA expression level reached the lowest level after 48 hours in the ACAT1 siRNA group (P < 0.05). In the ACAT1 siRNA group, compared with the blank control group and the negative control group, proliferation rate of PC-3 cell decreased, the numbers of invasive cell and migration cell reduced, the differences were statistically significant (P < 0.01). Compared between the blank control group and the negative control group, the differences in proliferation rate, invasion and migration ability of human prostate cancer PC-3 cells had no statistically significant (P > 0.05). Conclusion ACAT1 siRNA reduces proliferation, migration and invasion of PC-3 cells, which maybe provide a new idea and strategy for pathogenesis and treatment of prostate cancer.
2017 Vol. 14 (22): 4-7,15 [Abstract] ( 217 ) HTML (1 KB)  PDF (926 KB)  ( 56 )
8 Investigation on the stability of the Penicillin Sodium skin test solution under the modified preparation method
WAN Xia1 PAN Jing2 XIAO Wei3 ZHANG Yong1 WANG Huibing4
Objective To investigate the changes of related substance of Penicillin Sodium skin test solution after keeping at 2-8℃ environment for 4 h, in order to guide the use of Penicillin Sodium for injection. Methods High performance liquid chromatography was used to analyze the samples of Penicillin Sodium. The Red Classical C18 chromatographic column (5 μm, 150.0 mm×4.6 mm) was adopted, phosphate buffer, methanol and acetonitrile were used as mobile phase, with the flow rate of 1.0 mL/min. The detecting wave length was set at 225 nm, the column temperature was 34℃ and the injection volume was 20 μL. Results The content of impurities in Penicillin solution would increase after acid or base destruction and the new impurities would appear, while there were no new impurities appeared in the Penicillin solution after storage for 4 h. The content of impurity F in skin test solution of Penicillin Sodium samples had no significant changes before and after storage, while the contents of impurities A, B, C, D, E had certain changes, but the total content of impurities was not more than the corresponding main area of the control solution. Conclusion The modified preparation method simplifies the preparation process. The content of related substance of Penicillin Sodium skin test solution will increase after keeping for 4 h at 2-8℃, which increase the possibility of false positive result, therefore, Penicillin Sodium should be used during shorter time.
2017 Vol. 14 (22): 8-10,29 [Abstract] ( 178 ) HTML (1 KB)  PDF (790 KB)  ( 41 )
11 Effect of Shujin Jianyao Pills on ischiadic nerve and cytokine in serum of CCI rats
SHI Hongchao1 OU Huiyu2 HE Fenglei1 CHEN Dixin2 PENG Fuquan1 LI Ruipeng2 MO Guoqiang1
Objective To study the effect of Shujin Jianyao Pills on the pathological changes of ischiadic nerve and cytokine in the serum of CCI (chronic constriction injury) rats. Methods 112 SD rats were randomly divided into sham operation group, model group, Zhuanggu Guanjie Pill group, Paracetamol and Caffeine Tablets group, Shujin Jianyao Pill low dose group (1.10 g/kg), middle dose group (2.19 g/kg) and high dose group (4.38 g/kg), with 16 rats in each group. The right common sciatic nerve was exposed by blunt dissection, and four ligatures were tied loosely around the sciatic nerve. Sham operation referred to the procedure of exposing the nerve as above but without any nerve ligation. All groups were administrated with corresponding drugs by intragastric administration once a day for 28 days. After the last administration, the hindpaw withdrawal latency (HWL) of the rats, the contents of inflammatory cytokines including TNF-α, IL-1β, IL-6, PGE2 in the serum and the pathological changes of ischiadic nerve were detected. Results Compared to the sham operation group, in the model group, the HWL was shortened, ischiadic nerve was become swollen and vacuolar, the serum levels of IL-1β and IL-6 were increased (P < 0.05). Compared to the model group, in the Shujin Jianyao Pill groups, the HWL were extended, the pathological changes were ameliorated induced by CCI, and the IL-1β and IL-6 levels were decreased (P < 0.05). Conclusion Shujin Jianyao Pill can alleviate the histologic changes of sciatic nerve, inhibit the expression of inflammatory cytokines, which has therapeutic effect on traumatic neuralgia.
2017 Vol. 14 (22): 11-15 [Abstract] ( 175 ) HTML (1 KB)  PDF (1268 KB)  ( 146 )
16 Action of human umbilical cord mesenchymal stem cells by intra-arterial transplantation for treatment of type 2 diabetic Beagle dogs 
DONG Song WANG Hong LEI Lei SHI Han WANG Yizhong
Objective To investigate the therapeutic effects of human umbilical cord mesenchymal stem cells (UCMSCs) transplanted into a dog model of type 2 diabetes mellitus (T2DM) via the arterial intervention. Methods 24 Beagle dogs were randomly divided into UCMSCs treatment group (treatment group, n = 11), diabetic model control group (model group, n = 8) and normal control group (normal group, n = 5). Dogs in treatment and model control group were fed on high fat diet and intravenously injected with alloxan to establish T2DM models. Treatment group accepted insulin therapy and UCMSCs transplantation via arterial intervention. Model group only received insulin therapy, and normal group did not receive any treatment. Distribution of UCMSCs traced by CM-Dil was observed under fluorescence microscope. In addition, the three groups were compared with body mass, blood fat, fasting plasma glucose, insulin dosage, Area under the curve of C-peptide, insulin secretion index (HOMA-β), insulin resistance index (HOMA-IR) before and after therapy. Results The cells traced by CM-Dil mainly distributed in the pancreas and kidney. At 4 weeks after transplantation, fasting plasma glucose and insulin dosage were decreased gradually in treatment group , up to 12 weeks average daily insulin dose was declined from (27.94±1.86) U to (15.02±2.90) U. Compared with the model group, at 12 weeks triglyceride, low density lipoprotein cholesterol, HOMA-IR decreased (P < 0.05),  under the curve of C-peptide, HOMA-β increased in treatment group (P < 0.05). Conclusion The method of translation of UCMSCs by artery intervention not only maximum guarantee the stem cells quantity into the pancreas , but also has great effects on reversing glucose and lipid metabolism disorder, recovering islet β cell function, decreasing insulin dosage, and relieving insulin resistance.
2017 Vol. 14 (22): 16-20 [Abstract] ( 196 ) HTML (1 KB)  PDF (917 KB)  ( 43 )
21 Animal experimental study of Yinxieping in the treatment of psoriasis vulgaris
CHEN Douhong1 CHEN Shaoxiu1 WANG Chao1 WANG Jiankang1 DU Xiao1 WU Shengying2
Objective To establish animal models of Balb/c nude mice with psoriasis vulgaris, observe the effect of Yinxieping on double adjustable epidermal tissue protein (AREG), vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Methods 36 Balb/c nude mice were randomly divided into model control group and Yinxieping A group, Yinxieping B group, the three groups were all smeared imiquimod cream for  consecutive 8 d in skin of 4 sections in head and neck, upper limbs, torso and lower extremities of mice to built the model. At the dose of 8th day after modeling, Yinxieping A group, Yinxieping B group were treated by gavage for 10 d with 0.1 g/kg and 0.5 g/kg dose, model group was injected into the same amounts saline. At the treatments 8th, 10th day, psoriasis area and severity index (PASI) was used to evaluated the animal head neck, upper limbs, torso and lower extremities 4 skin lesions; ELISA was uesd to determine contents of AREG, KGF, VEGF and vascular endothelial growth factor receptor 1 (VEGFR1) and other indicators in epidermal tissue homogenates in a fixed quantity. Results PASI scores of Yinxieping A group, Yinxieping B group and model control group at modeled 8th day were compared, the differences were not statistically significant (P > 0.05), when treated by gavage at 10th day, compared with the model control group and themsavies at modeled 8th day, PASI scores of Yinxieping A group, Yinxieping B group decreased significantly (P < 0.05), the expression of AREG, KGF, VEGF and VEGFR1 in epidermal tissue homogenates was lower (P > 0.05). Yinxieping A group and Yinxieping B group were compared, the differences were not statistically significant (P > 0.05). Conclusion Yinxieping can decrease contents of AREG, KGF, VEGF in epidermal tissue homogenate, and it may block the combination of VEGF and VEGFR1, which has positive effects in the inhibition of epidermal keratinocyte lesions and subcutaneous tissue angiogenesis, reducing the subjects change in animal with psoriasis to play parts in pathological intervention.
2017 Vol. 14 (22): 21-24 [Abstract] ( 278 ) HTML (1 KB)  PDF (603 KB)  ( 73 )
25 Metabolomic study of protective effect of aucubin for photoaging ESF-1 cells induced by UVA
LI Xue1 LEI Xuexue1 XU Hongdan2▲ ZHOU Jing1▲
Objective To analyze the mechanism and effective target of aucubin intervene photoaging ESF-1 cells by high through-put cell metabolomics. Methods Cell photoaging model was established by UVA cell injury method, then given low medium and high dose aucubin DMEM medium for the intervention. The activities and contents of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde (MDA) were detected for the evaluation of oxidative damage and aucubin treatment effect. Based on above analysis and high resolution mass spectrometry, the high throughput metabolic footprint analysis was conducted in the normal cells, photoaging ESF-1 cells and aucubin intervention cell. Massive metabolic data were analzyed with dimension reduction process and multivariate statistics by model discriminating technique. Results Compared with the blank group, the activities of SOD, GSH-Px, CAT significantly decreased and contents of MDA significantly increased in the model group (P < 0.01). Compared with the model group, the activities of SOD, GSH-Px, CAT increased and contents of MDA decreased in the medication gorups, of which, in the medium and high doses medication groups the differences were statistically significant (P < 0.05 or P < 0.01). There were significant differences in the metabolism between the normal cells and the photoaging ESF-1 cells, and the disturbance of metabolism was mainly concentrated in arachidonic acid metabolism, glutathione metabolism. 6 core biomarkers were identified by focusing analysis, and these small molecular metabolites were callback after the administration of aucubin. Conclusion Aucubin has a favorable effect on photoaging, and the mechanism may be related to arachidonic acid metabolism. Besides, the present investigation provides new ideas and methods for early prevention and drug development.
2017 Vol. 14 (22): 25-29 [Abstract] ( 215 ) HTML (1 KB)  PDF (1159 KB)  ( 76 )
30 Determination of anti-FXa potency in heparin sodium and inositol nicotinate cream by micro-chromogenic substrate method
LI Xiang MA Hongda WU Qiong HOU Mingxiao▲
Objective To establish a method for determination of anti-FXa potency in heparin sodium and inositol nicotinate cream. Methods The emulsion was broken. The anti-FXa potency was determined by micro-chromogenic substrate method and biological test statistics method. Results The determination method was established for anti-FXa potency in heparin sodium and inositol nicotinate cream. The linear range of anti-FXa was during 0.0250-0.1020 IU/mL (r=0.996). The average recovery in three levels was 99.5% (RSD=1.92%, n=9). The ratio of potency was during 0.9-1.1 for anti-FXa and anti-FⅡa. Conclusion The method is accurate and reproduceable. It can be used for the determination of anti-FXa potency in order to control the quality of heparin sodium and inositol nicotinate cream.
2017 Vol. 14 (22): 30-32 [Abstract] ( 168 ) HTML (1 KB)  PDF (541 KB)  ( 95 )
33 Quality assessment of Cortex Periplocae by HPLC fingerprint and UV quantitative analysis
LI Ruixue1 WU Fei1，2 ZHANG Jiquan1，2 ZHAO Pei3 FENG Yi1，2
2017 Vol. 14 (22): 33-36,46 [Abstract] ( 203 ) HTML (1 KB)  PDF (753 KB)  ( 101 )
37 Determination of residual solvents in Cefpodoxime Proxetil
ZHANG Qingguo ZHOU Fang GUO Shuxing HE Ming WANG Limin CHEN Dianqing
Objective To establish a suitable method for determination of residual solvents in Cefpodoxime Proxetil. Methods Agilent DB-624 capillary column (30.0 m×0.25 mm×1.4 μm) was used, with FID detector, thirteen residual solvents (acetaldehyde, methanol, ethanol, isopropanol, dichloromethane, isopropyl ether, butanone, ethylacetate, tetrahydrofuran, cyclohexane, pyridine, toluene, butyl acetate) in Cefpodoxime Proxetil were determinated and analyzed by external standard method, internal standard method, standard addition method and correction factor standard addition method. Results The results showed that all residual solvents could be separated, and good linear relationships were obtained in their respective concentration ranges in the methods (r = 0.9980-1.0000), the detection limits were between 0.0250 to 0.3284 μg/mL. And significant differences recovery rates between all kinds of residual solvents, with the lowest recovery rates in acetaldehyde (39.7644%-42.1885%) and with the highest recovery rates in cyclohexane (125.3627%-132.2517%). Conclusion The standard addition method is the most suitable determination method for the residual solvents in Cefpodoxime Proxetil.
2017 Vol. 14 (22): 37-41 [Abstract] ( 222 ) HTML (1 KB)  PDF (716 KB)  ( 99 )
42 Quality standard analysis and research for Jinchanzhiyang Granules
JIA Lin JIANG Long
Objective To establish the quality standard of Jinchanzhiyang Granules. Methods The contents of Gardenia jasminoides Ellis, Sophora flavescens, Phellodendron amurense Rupr. in Jinchanzhiyang Granules were identified by using thin layer chromatography (TLC) method. The contents of Berberine, Osthole were determined by high performance liquid chromatography (HPLC) method. Results TLC had strong specificity and high separation. Negative control without interference. The linear range was 0.00648-0.1296 μg for Berberine (r=0.9996) and 0.0045-0.1125 μg for Osthole (r=0.9996) respectively. The average recovery was 99.16% (RSD=1.21%), 99.22% (RSD=1.33%) respectively. the content of Berberine and Osthol was 0.1135, 0.0310 mg/g respectively. Conclusion The method is accurate and simple, and can be used for the quality control of Jinchanzhiyang Granules.
2017 Vol. 14 (22): 42-46 [Abstract] ( 180 ) HTML (1 KB)  PDF (774 KB)  ( 68 )
47 Pathogenesis of ISNA and it′s research progess of the prevention and control of traditional Chinese and western medicine
LI Danyun1 WANG He2 SI Chunying2 LUO Minghua2 CHEN Yushan2 GUAN Huaimin2
Percutaneous coronary intervention has become the important means on the therapy of coronary heart disease. But, the current clinical problems solved are postoperative restenosis and late stent thrombos is which can cause serious adverse cardiovascular events. Studies have found that in-stent neoatherosclosis is an  important reason of ISR and LST. For the prevention and treatment of ISNA is the entry point to improve the long-term effect of coronary stent at present. In this paper, the pathogenesis of neonatal atherosclerosis and the prevention and treatment of Chinese and western medicine were discussed.
2017 Vol. 14 (22): 47-50 [Abstract] ( 194 ) HTML (1 KB)  PDF (490 KB)  ( 124 )
51 Advanced in research of poly (ADP-ribose) polymerase in alcoholic fatty liver disease
HUANG Shishun LIU Yang ZHANG Bing LI Xin BAO Zhiwei WANG Zhigang▲
Alcoholic fatty liver disease (AFLD) caused by chronic drinking which is one of liver disease. Chronic alcohol consumption increased hepatic fat anabolism and decreased lipolysis which caused hepatic fat accumulation. Moreover, chronic alcohol exposure induced steatohepatitis, hepatic fibrosis and cirrhosis. But the pathological mechanism is not yet clear. Poly (ADP-ribose) polymerase (PARP) is a intracellular DNA repairase which participated in intracellular gene transcription, DNA repair, genome stability and cell apoptosis and necrosis. PARP is a consumer of intracellular nicotinamide adenine dinucleotide (NAD+). The activation of PARP regulated the intracellular NAD+ level. Chronic alcohol consumption induced PARP activity which play an important role in the development of alcohol fatty liver disease. The inhibition of PARP or gene deletion of PARP in mice attenuated hepatic fat accumulation, inflammatory, oxidative stress and hepatic apotosis and necrosis by chronic alcohol exposure. This review focus on the advanced research of PARP in alcoholic fatty liver disease.
2017 Vol. 14 (22): 51-54 [Abstract] ( 134 ) HTML (1 KB)  PDF (457 KB)  ( 91 )
55 Progress of gene and proteomics research of leukoaraiosis
ZHANG Yu FENG Yuan GUO Dazhi PAN Shuyi
Leukoaraiosis is a descriptive term for rarefaction of the white matter, showing up as nonspecific changes in the periventricular and sub-cortex white matter frequently seen on CT and MRI. Currently researches focus on its risk factors, clinical manifestations, treatment, and so on. Most studies have shown that leukoaraiosis is caused by low perfusion of white matter area related to chronic ischemia. Studies also demonstrate the relationship between leukoaraiosis and certain changesof human genes and protein. In this paper, the newest genomics and proteomics researches of leukoaraiosis are reviewed.
2017 Vol. 14 (22): 55-58 [Abstract] ( 166 ) HTML (1 KB)  PDF (465 KB)  ( 60 )
59 Effect of follicle flushing on embryonic developmentoflong protocol controlled ovarian hyperstimulation in vitro fertilization
JING Xia1 FENG Zhonghai2 WEI Bing1 CHEN Bi1 PENG Cailing1 ZOU Lin1 LI Yunqing1 HUANG Riyan1 MA Tianzhong1▲
Objective To evaluate the effects of follicle flushing on embryonic development in patients of long protocol controlled ovarian hyperstimulation in vitro fertilization. Methods From July 2014 to June 2016, in Reproductive Medicine Center, Affiliated Hospital of Guangdong Medical University, the clinical data of 343 patients underwent in vitro fertilization (IVF) were analyzed retrospectively. Among them，99 patients treated with G-MOPs were set as follicle flushing group, and 244 patients sucked direethy folliculer fluid  were as control group. The oocyte retrieval rate, fertilization rate, 2PN cleavage rate, good quality embryo rate, blastocyst rate, available embryo rate, clinical pregnancy rate, implantation rate, abortion rate of two groups were compared. Results The available embryo rate, good quality embryo rate and blastocyst rate in follicle flushing group were higher than those in control group, the differences were statistically significant (P < 0.05), and other clinical and lab indication also checking had no statistical difference between the two groups (P > 0.05). Conclusion Follicle flushing is not necessary to increase the oocyte retrieval rate, but can increase the available embryo rate, good quality embryo rate and blastocyst rate, it showes a increased tendency gradually of clinical pregnancy rate and implantation rate.
2017 Vol. 14 (22): 59-62 [Abstract] ( 144 ) HTML (1 KB)  PDF (569 KB)  ( 39 )
63 Clinical study of posterior lumbar interbody fusion using pedicle screw system under intervertebral disc
ZHU Xiankang ZHONG Jiayun▲ LI Tengbiao LIN Xiaquan ZHAO Debing HUANG Qiyu HOU Xinhe
Objective To explore the clinical effect of intervertebral fusion after lumbar intervertebral disc fusion under the intervertebral disc. Methods From February 2010 to March 2012, in Yulin Red Cross Hospital, the clinical data of 92 patients with lumbar disc herniation were retrospectively analyzed. 46 patients treated with pedicle screw channel posterior lumbar interbody fusion by intervertebral disc were as observation group, 46 patients treated with traditional open posterior intervertebral disc extraction and pedicle screw fixation were as control group. The intraoperative blood loss, operation time, hospitalization time, pain score, intervertebral height, clinical improvement rate and satisfaction rate of the two groups were compared. Results The intraoperative blood loss, operation time and length of hospitalization in the observation group were significantly lower than those in the control group, the differences were statistically significant (P < 0.05). After the treatment, the pain score, vertebral space of the observation group were less than those of the control group, the differences were statistically significant (P < 0.05). The  clinical improvement rate in the observation group was higher than that in the control group, the differences were statistically significant (P < 0.05). The total satisfaction rate in the observation group was higher than that in the control group, the differences were statistically significant (P < 0.05). Conclusion The establishment of posterior lumbar intervertebral fusion with intervertebral disc through intervertebral disc is effective and effective, and it has less hemorrhage, less incision and quick recovery after operation. It is worthy of study and popularization.
2017 Vol. 14 (22): 63-65,88 [Abstract] ( 154 ) HTML (1 KB)  PDF (1039 KB)  ( 78 )
66 Comparison the effect of balloon dilatation alone and combination with drug eluting stent implantation in treatment of infrapopliteal arterial occlusive disease
WU Zhongjian GU Yongquan▲ QI Lixing GUO Lianrui CUI Shijun LI Jianxin YANG Shengjia TONG Zhu
Objective To compare and analyze the efficacy of balloon dilatation alone and combination with drug eluting stent implantation in the treatment of infrapopliteal arterial occlusive disease. Methods The clinical data of 126 patients (139 limbs) with infrapopliteal arterial occlusive disease and underwent the endovascular treatment from March to 2013 and October 2015 in Xuanwu Hospital, Capital Medical University were retrospectively analyzed. All the cases received balloon dilatation therapy at first, and 32 limbs among them received the drug-eluting stent implantation in infrapopliteal artery. After the surgery, the improvement of symptoms, the change of ankle brachial indexes, changes of vascular ultrasonic images, the limb salvage rate and recent patency rate and so on were observed. Results 8 patients (10 limbs) failed, and the ankle brachial index (ABI) of successful cases increased from  before surgery (0.39±0.13) to 3 days after the surgery (0.82±0.16) (P < 0.05). Overall infrapopliteal arterial patency rate  6 months after the operation was 83.5% (101/121), and 12 months after operation was 69.5% (57/82), the patency rates of those with balloon dilatation alone were 83.3% (75/90) and 71.0% (44/62) respectively, the patency rates of those with stent implantation were 83.9% (26/31) and 65.0% (13/20) respectively, there were no statistical difference in efficacy between balloon dilation alone and stent implantation 6 months and 12 months after operation (P > 0.05 ). Conclusion Both endovascular interventional therapy with drug eluting stent implantation and balloon dilatation alone can be used to treat infrapopliteal arterial occlusive disease and they are with similar short-term efficacy. If there are severe complications that affect the blood flow in the treatment of infrapopliteal arterial disease, stent implantation should be considered to improve limb blood flow, avoid the complications and improve the limb salvage rate.
2017 Vol. 14 (22): 66-68 [Abstract] ( 158 ) HTML (1 KB)  PDF (561 KB)  ( 101 )
69 Influene of Aspirin combined with Clopidogrel on  nerve function and ability of daily life in acute ischemic cerebrovascular disease patients
MENG Qingling
Objective To discuss the influence about nerve function and the ability of daily life of Aspirin combined with Clopidogrel treated in the patients with acute ischemic cerebrovascular disease. Methods From August 2015 to August 2016, in Second People's Hospital of Liaocheng City, 110 patients with acute ischemic cerebrovascular disease were selected, they were divided into the control group and observation group according to the random number table, with 55 cases in each group. The patients in control group were given Clopidogrel therapy, patients in observation group were given Clopidogrel combined with Aspirin treatment. NIHSS score, Barthel index, modified Rankin scale, and evaluation of the quality of life of two groups were compared. Results Compared with before treatment, the NHISS score reduced, the Barthel index increased, modified Rankin scale reduced in the two groups after treatment, and the improvement of the observation group were more significant than the control group after treatment, the differences were statistically significant (P < 0.05). Compared with before treatment, the physical function, role function, emotional function, cognitive function and social function score of the two groups after treatment increased, the improvement of the observation group were more significant than that indexes of the control group after treatment, the differences were statistically significant (P < 0.05). Conclusion The combination therapy of Aspirin with Clopidogrel treated for the acute ischemia cerebral vessels disease can improve nerve function and daily life ability, reduce the quality of life.
2017 Vol. 14 (22): 69-71,128 [Abstract] ( 147 ) HTML (1 KB)  PDF (938 KB)  ( 81 )
72 Risk factor analysis of postoperative infection for spinal posterior
HUANG Xiuyu
Objective To approach risk factors of postoperative infection for spinal posterior. Methods From March 2014 to March 2016, in  Second People's Hospital of Neijiang City, the clinical data of 1170 spinal posterior postoperative infection patients were selected, they were divided into two groups by infection or not, infection group 300 cases and no infection group 870 cases. The preoperative risk factors, intraoperative factors, postoperative factors and infection rate of two groups were observed, the multi-factors of postoperative infection for spinal posterior were observed. Results The myodynamia≤3 grade, immunologic decline, acute or chronic infection, operation time≥180 min, amount of bleeding>600 mL, allograft, no correct using  antibiotics, gatism or retention catheterization before operation, after operation ≥3 d, shift in to ICU after operation, number of drainage tube, indwelling drainage tube>5 d of infection group were lower than no infection group, the differences were statistical significance (P < 0.05 or P < 0.01). The acute or chronic infection, BMI>30 kg/m2, shift in to ICU after operation were risk factors of postoperative infection for spinal posterior (P < 0.05). Conclusion The acute or chronic infection, BMI>30 kg/m2, shift to ICU after operation are risk factors of postoperative infection for spinal posterior.
2017 Vol. 14 (22): 72-75 [Abstract] ( 167 ) HTML (1 KB)  PDF (576 KB)  ( 61 )
76 Effect of acupuncture combined with general anesthesia on cognitive function and related inflammatory factors in elderly patients with tumor resection
LIU Zhi TENG Yongjie
Objective To investigate the effect of acupuncture combined with general anesthesia on cognitive function and related inflammatory factors in elderly patients underwent tumor resection. Methods A total of 98 elderly patients underwent tumor resection in the First Affiliated Hospital of Hu′nan University of Traditional Chinese Medicine from June 2015 to December 2016 were enrolled. The patients were divided into two groups according to the random number table method, each group had 49 cases. Patients in the control group were treated with general anesthesia. Patients in the observation group were treated with acupuncture combined with general anesthesia. All patients were scored by mini-mental state examination (MMSE) at 1 day before operation, 1 day after operation and 3 days after operation. The incidence of postoperative cognitive dysfunction (POCD) was calculated. The levels of serum TNF-α, IL-1β and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) before operation, at operation completed, 24 h and 48 h after operation. Results The MMSE scores of the observation group were significantly higher than those of the control group at 1 day and 3 days after operation, the incidence rates of POCD were lower than those of the control group, the differences were statistically significant (P < 0.05). The levels of TNF-α, IL-1β and IL-6 in the two groups at operation completed, 24 h and 48 h after operation were significantly higher than those before operation, and the observation group were lower than those of the control group, the differences were statistically significant (P < 0.05). Conclusion Acupuncture combined with general anesthesia can reduce the incidence of POCD in elderly patients underwent tumor resection. The mechanism may be related to the decrease of the levels of serum TNF-α, IL-1β and IL-6 in patients.
2017 Vol. 14 (22): 76-79 [Abstract] ( 164 ) HTML (1 KB)  PDF (459 KB)  ( 50 )
80 Effect of application of C-arm guidance combined with electrical stimulation in trigeminal neuronal radiofrequency coagulation therapy for primary maxillary neuralgia
ZHANG Qianxi1,2 SHEN Wen1▲
Objective To explore the effect of application of C-arm guidance combined with electrical stimulation in trigeminal neuronal radiofrequency coagulation therapy for primary maxillary neuralgia. Methods 50 cases of patients with primary trigeminal maxillary neuralgia of the Ⅱ branch treated in the Affiliated Huai′an Hospital of Xuzhou Medical University from January 2012 to December 2015 were selected, and randomly divided into the observation group and the control group, each group had 25 cases. All patients were treated by C-arm guided modified Hartel approach through foramen ovale into the trigeminal ganglion. The control group was treated with radiofrequency coagulation guided by C-arm, the observation group was combined with electrical stimulation directing. The visual analogue scale (VAS), excellent rate, recurrence rate and complications of the two groups were follow-up in preoperative and postoperative instantly, 1 month, 3 months, 6 months and 12 months after operation. Results Both the control group and the observation group were followed up completely. The VAS scores of postoperative instantly, 1 month, 3 months, 6 months and 12 months after operation in the observation group were obviously lower than those of the control group, and the differences were statistically significant (P < 0.05). The excellent rates of the observation group were higher and the recurrence rates were lower than those of the control groups, with statistically significant differences (P < 0.05). The incidence of numbness in mandibular nerve zone of Ⅲ branch was lower in the observation group than that in the control group, with statistically significant difference (P < 0.05). There was no significant difference in complications between the two groups (P > 0. 05). Conclusion Compared with C-arm guidance, combined with electrical stimulation has higher location accuracy, better clinical effects and lower recurrence rate.
2017 Vol. 14 (22): 80-83 [Abstract] ( 209 ) HTML (1 KB)  PDF (547 KB)  ( 108 )
84 Primary exploration on international cooperation and exchange platform construction of traditional Chinese medicine under the background of “One Belt and One Road” initiative
SUN Yinping HU Kaiwen WANG Yiguang AN Chao ZHANG Yunling
In recent years, the global development of traditional Chinese medicine (TCM) enters a new normal state, the growth of international exchange of TCM is slowing down. Under the background of "One Belt and One Road" initiative, the global development of TCM ushers in new opportunity, which also brings new challenges for the international cooperation and exchange model of TCM hospitals. This paper analyzes the problems and difficulties of TCM internationalization, combining with the relevant practical experience of international cooperation and exchange in a certain grade 3A TCM hospital, which were carrying out the collaboration of overseas central construction of traditional medicine, medical service, scientific research cooperation and talent cultivation, expanding the depth and breadth of communication constantly, improving the levels of cooperation and exchange of TCM, thus put forward some suggestions for constructing international cooperation and exchange platform of TCM: taking the cultural transmission of TCM as foundation, coordinated development of TCM standardization and intellectual property strategy as premise, cultivating the international compound talents of TCM as key points, actively promoting the scientific research and technical cooperation of TCM during the international medical institutions, building long-term mechanism of international cooperation and exchange of TCM with international friendly, to form more close and pragmatic partnership.
2017 Vol. 14 (22): 84-88 [Abstract] ( 195 ) HTML (1 KB)  PDF (781 KB)  ( 89 )
89 Clinical effect of invigorating the spleen and tonifying the kidney combined with acupuncture in the treatment of knee osteoarthritis
DONG Yuxi CHEN Yanming WANG Qiuhong SHANG Lixia SHI Guirong LIU Xinyan CHEN Ting
Objective To observe the clinical effect of invigorating the spleen and tonifying the kidney combined with knee acupoints acupuncture in the treatment of knee osteoarthritis in the type of deficiency of both the spleen and the kidney, dampness infusion into bone and joints. Methods One hundred and twenty patients with knee osteoarthritis in the type of spleen and kidney deficiency, dampness infusion into bone and joints, who were treated in the Outpatient Department of Beijing Changping Hospital of Traditional Chinese Medicine from January 2015 to December 2016, were selected and randomly divided into two groups by block randomization method, each group had 60 cases. The treatment group was treated with acupuncture by invigorating the spleen and tonifying the kidney (taking the abdominal acupoints of Zhongwan, Xiawan, Qihai, Guanyuan, Daheng) combined with knee acupoints (Heding, Liangqiu, Xuehai, Yinlingquan, Yanglingquan), the control group was taken the method of acupuncture treatment of simple knee acupoints. After 1 course of treatment, the visual analogue score (VAS), knee circumference, the scores of Western Ontario McMaster University osteoarthritis index (WOMAC) (including joint stiffness, daily activities and social function) of the two groups before and after treatment were compared; 3 months after the end of treatment, the VAS scores and knee circumference were followed up and compared with the results before and after treatment. Results The scores of knee stiffness, joint daily activities and social function of the two groups after treatment were significantly lower than those before treatment, and the treatment group was lower than the control group, the differences were all statistically significant (all P ＜ 0.05). Compared with before treatment, the VAS scores of the two groups were decreased after treatment and follow-up, and the treatment group was lower than the control group, the differences were all statistically significant (all P ＜ 0.05). Compared with after treatment, the VAS scores in the treatment group at follow-up was decreased (P ＜ 0.05), and the VAS scores in the control group at follow-up was slightly decreased, but there was no statistically significant difference (P ＞ 0.05). The knee circumference of the two groups after treatment was decreased obviously compared with that before treatment (all P ＜ 0.05), there was no significant difference between the two groups (P ＞ 0.05). The knee circumference of the two groups at follow-up was lower than that before treatment (all P ＜ 0.05), and the treatment group was significantly lower than the control group (P ＜ 0.05); compared with after treatment, the knee circumference of the treatment group at follow-up was decreased, and the knee circumference of the control group was increased (all P ＜ 0.05). Conclusion The combination of acupuncture the abdominal acupoints with the knee acupoints to treat the knee osteoarthritis in the type of deficiency of both the spleen and the kidney, dampness infusion into bone and joints using the method of invigorating the spleen and tonifying the kidney can significantly reduce the pain and swelling, and improve daily joint function. The curative effect is stable, which is better than the treatment of simple knee acupoints.
2017 Vol. 14 (22): 89-92 [Abstract] ( 176 ) HTML (1 KB)  PDF (780 KB)  ( 52 )
93 Research on physical health promotion of urban white-collar in Shanghai based on the view of constitution of traditional Chinese medicine
XU Yangcai1 LI Jixia2 WU Zhikun1 GONG Bomin1 XU Huaping1
Objective To explore and analyze the influence of TCM type judgment and TCM preserve health according to different constitution on the physical health of urban white-collars from the perspective of traditional Chinese medicine constitution. Methods By using the methods of literature review, questionnaire survey and expert consultation, a total of 294 investigators in Shanghai Zhangjiang Hi-Tech Park were randomly selected to investigate the physical health status from March to July 2016. Results Most of urban white-collars in Shanghai had biased physique of traditional Chinese medicine, and mostly belong to the virtual body, which were basically consistent with their health status of self-evaluation results. With the increase of age, the proportion of Shanghai urban white-collars in sub-health state and disease state also increased year by year. The main factors influencing the physical health were unhealthy lifestyles, such as lack of sports, poor diet and unhealthy sleeping habits, stressful working life and so on. There were significant differences in the distribution of physical constitution with different exercise habits of urban white-collars in Shanghai (χ2=29.46, P ＜ 0.05). Conclusion According to the principle of preserve health according to different constitution, selecting reasonable, scientific sports and health preservation methods on the basis of individual constitution type can adjust the biased physique and achieve the state of yin and yang in equilibrium, physical and mental health.
2017 Vol. 14 (22): 93-96 [Abstract] ( 179 ) HTML (1 KB)  PDF (775 KB)  ( 259 )
97 Observation on the effect of antagonistic acupuncture combined with modified constraint-induced movement therapy in upper limb spasticity after acute cerebral infarction
LI Baoji1 HOU Qiang1 WANG Xiaoning2▲
Objective To investigate the effect of antagonistic acupuncture combined with modified constraint-induced movement therapy in upper limb spasticity after acute cerebral infarction. Methods One hundred and twenty patients with upper limb spasticity after acute cerebral infarction admitted to Department of Rehabilitation, Baoji Hospital of Traditional Chinese Medicine from March 2014 to May 2015 were selected and divided into observation group and control group by random number table method, with 60 cases in each group. The observation group was given antagonistic acupuncture combined with modified constraint-induced movement therapy, the control group was given single modified constraint-induced movement therapy. The efficacy was evaluated by simple Fugl-Meyer assessment (FMA) and modified Ashworth muscular tension grading, the improvement of this two methods for the upper limb spasticity state after acute cerebral infarction. Results After treatment, the total effective rate of modified Ashworth muscular tension grading efficacy in observation group was higher than that of control group, the difference was statistically significant (P ＜ 0.05). The FMA scores of the two groups after treatment were higher than those before treatment (P ＜ 0.05),  and the FMA scores of observation group after treatment were higher than those of control group, the differences were statistically significant (P ＜ 0.05). Conclusion Antagonistic acupuncture combined with modified constraint-induced movement therapy can significantly improve the state of upper limb spasticity after acute cerebral infarction, which is better than single use of modified constraint-induced movement therapy, and it is easy to operate for patients and worthy of clinical promotion and application.
2017 Vol. 14 (22): 97-100 [Abstract] ( 181 ) HTML (1 KB)  PDF (777 KB)  ( 45 )
101 Clinical research of Qufeng Lishi Mixture in treating fengshi yunfu-type atopic dermatitis
LI Xingzi1 XU Ping1 PAN Weidong2 ZHANG Huimin1▲
Objective To discuss the clinical efficacy of Qufeng Lishi Mixture in treating fengshi yunfu-type atopic dermatitis. Methods Seventy-two patients with fengshi yunfu-type atopic dermatitis admitted to Department of Dermatology of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from September 2015 to February 2016 were selected and divided into experimental group and control group by random number table method, with 36 cases in each group. The experimental group was treated with oral adminstration of 30 mL Qufeng Lishi Mixture, twice a day; the control group was treated with oral adminstration of 10 mg Cetirizine Hydrochloride, once a day. Both groups were given Chloramphenicol Dexamethasone Cream for external use, twice a day. The course of the two groups was both 4 weeks. The changes of visual analogue scale (VAS), the percent of itching time (PIT), eczema area and severity index (EASI) integral before and after the treatment and the clinical efficacy in the two groups were observed. Results After treatment, the VAS score, PIT value and EASI integral in two groups were all lower than those before treatment (P ＜ 0.01). The PIT value and EASI integral in the experimental group after treatment were lower than those of control group (P ＜ 0.01). The total effective rate of experimental group and control group was 82.4% and 64.5% respectively, but there was no statistically significant difference between the two groups (P ＞ 0.05). The cured and markedly effective rate of experimental group (50.0%) was significantly higher than that of control group (22.6%) (P ＜ 0.05). Conclusion Qufeng Lishi Mixture in the treatment of fengshi yunfu-type atopic dermatitis has good clinical efficacy, which can significantly improve the degree of pruritus and skin lesions.
2017 Vol. 14 (22): 101-104 [Abstract] ( 175 ) HTML (1 KB)  PDF (827 KB)  ( 145 )
105 Clinical study on acupuncture at Bahuang acupoints in the treatment of acute attack of migraine
LYU Pin TI Guixiang WANG Liyun LYU Weihua▲
Objective To observe the clinical effect of acupuncture at Bahuang acupoints in the treatment of acute attack of migraine. Methods Sixty patients with migraine acute attacks in Outpatient Department of Prevention and Healthcare, Special Outpatient of Migraine, some Outpatient Departments of Acupuncture, Guang'anmen Hospital, China Academy of Chinese Medical Sciences from January 2016 to January 2017 were selected, and they were divided into two groups according to the random number table, with 30 cases in each group. The treatment group was treated with acupuncture at Bahuang acupoints, and the control group was treated with acupuncture at regular acupoints, each group for 30 min. After treatment for 1, 2 , 4, 24 hours, the visual analogue scale (VAS) of headache and cerebral blood flow velocity after treatment of the two groups were observed, and the curative effect of the two groups was evaluated. Results After treatment, the VAS scores of headache and cerebral artery blood flow velocity in the two groups were lower than those before treatment, the differences were statistically significant (P ＜ 0.05), while there were no statistically significant differences between the two groups after treatment (P ＞ 0.05). After treatment for 2 hours, the number of 50% relieved degree of headache in the treatment group was 24 cases (80.0%), which of the control group was 22 cases (73.3%), the difference between the two groups was not statistically significant (P ＞ 0.05). The routine blood and urine routine, and liver and kidney function of the two groups had no obvious changes before and after treatment, and the safety of acupuncture was guaranteed. Conclusion Acupuncture at Bahuang acupoints can improve the clinical symptoms of patients with migraine, and it is safe and easy to operate.
2017 Vol. 14 (22): 105-108 [Abstract] ( 206 ) HTML (1 KB)  PDF (776 KB)  ( 77 )
109 Clinical effect of Supplementing Qi and Nourishing Yin, Activating Blood and Dredging Collaterals Prescription in the treatment of diabetic nephropathy
LU Cong1 ZHAO Jinxi2▲
Objective To observe the clinical effect of Supplementing Qi and Nourishing Yin, Activating Blood and Dredging Collaterals Prescription in the treatment of diabetic nephropathy and the influence on inflammatory factors. Methods Eighty cases of patients with diabetic nephropathy admitted to Shunyi Hospital of Traditional Chinese Medicine from June 2015 to February 2017 were selected as research objects, and they were randomly divided into observation group (42 cases) and control group (38 cases). Both groups were given clinical conventional treatment, on basis of which, the control group was given Valsartan Capsules 80 mg every time, once a day. The observation group was added with Supplementing Qi and Nourishing Yin, Activating Blood and Dredging Collaterals Prescription, one dose a day. Both groups took 12 weeks as a course. The clinical effect and the aggregate scores of main syndromes of traditional Chinese medicine of the two groups were observed and compared. The changes of renal function indexes [glomerular filtration rate (GFR), blood urea nitrogen (BUN), serum creatinine (Scr), microalbuminuria (mAlb), 24-hour urinary protein quantitation] of the two groups before and after treatment were recorded. Results The clinical effect of observation group was better than that of control group, the difference was statistically significant (P ＜ 0.05). After treatment, the aggregate scores of main syndromes of traditional Chinese medicine in observation group and control group [(10.31±2.59), (12.76±3.23) points] were all lower than before treatment [(29.11±8.13), (30.37±9.46) points], and the aggregate scores of main syndromes of traditional Chinese medicine of observation group after treatment were lower than those of control group, the differences were all statistically significant (all P ＜ 0.05). After treatment, the levels of GFR in observation group and control group [(80.54±11.36), (75.86±9.72) mL/min] were all higher than before treatment [(71.38±8.59), (70.25±8.33) mL/min], levels of BUN [(6.14±0.52), (6.83±0.76) mmol/L], Scr [(91.5±16.2), (102.6±19.8) μmol/L], mAlb [(40.04±5.26), (53.22±7.39) mg/L], 24-hour urinary protein quantitation [(0.73±0.05), (0.95±0.09) g/24 h] were all lower than before treatment [(7.81±0.95), (7.92±1.06) mmol/L; (110.4±20.7), (113.6±21.5) μmol/L; (89.03±18.15), (86.56±17.11) mg/L; (1.57±0.16), (1.59±0.23) g/24 h], the differences were all statistically significant (all P ＜ 0.05), and the level of GFR after treatment in observation group was higher than that of control group, the levels of BUN, Scr, mAlb, 24-hour urinary protein quantitation were lower than those of control group, the differences were all statistically significant (all P ＜ 0.05). Conclusion Supplementing Qi and Nourishing Yin, Activating Blood and Dredging Collaterals Prescription in the treatment of diabetic nephropathy can significantly improve clinical symptoms, reduce renal injury, which is worthy of clinical promotion and application.
2017 Vol. 14 (22): 109-112 [Abstract] ( 173 ) HTML (1 KB)  PDF (778 KB)  ( 78 )
113 Efficacy of Ligustrazine Injection on endothelial function, coagulation fibrinolysis system in elderly patients with acute exacerbations chronic obstructive pulmonary disease
WEI Feng
Objective To evaluate the efficacy of Ligustrazine Injection on endothelial function, coagulation fibrinolysis system in elderly patients with acute exacerbations chronic obstructive pulmonary disease (AECOPD). Methods One hundred and twenty elderly patients were AECOPD admitted to Affiliated Hospital of Youjiang Medical University for Nationalities from August 2015 to August 2016 were selected and divided into observation group and control group by random number table method, with 60 cases in each group. The control group was treated with conventional therapy, and the observation group was treated with Ligustrazine Injection combined with conventional therapy. The forced expiratory volume in the first second (FEV1), forced vital capacity (FVC), FEV1/FVC, fibrinogen (FIB), prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT), endothelin-1 (ET-1)and nitric oxide (NO) of the two groups before and after treatment were compared. Results After treatment, the FEV1, FVC, FEV1/FVC and NO of observation group were significantly higher than those before treatment (P ＜ 0.05), the PT, APTT, TT, FIB and ET-1 of observation group were significantly lower than those before treatment (P ＜ 0.05). The ET-1 of control group was significantly lower than that before treatment (P ＜ 0.05), and the NO of control group was significantly higher than that before treatment (P ＜ 0.05). The FEV1, FVC, PT, APTT, TT and FIB of control group were not significantly different compared with those before treatment (P ＞ 0.05). After treatment, the FEV1, FVC, FEV1/FVC and NO of observation group were significantly higher than those of control group (P ＜ 0.05), the PT, APTT, TT, FIB and ET-1 of observation group were significantly lower than those of control group (P ＜ 0.05). Conclusion Ligustrazine Injection can adjust the endothelial function and blood high condensation state induced by AECOPD, and improve the lung function.
2017 Vol. 14 (22): 113-116 [Abstract] ( 133 ) HTML (1 KB)  PDF (776 KB)  ( 93 )
117 Clinical application of vascular ultrasound in carotid endarterectomy
FU Wenyan LI Shuling
Objective To invastigate the clinical application value of vascular ultrasound in carotid endarterectomy (CEA). Methods Fifty-one patients with carotid atherosclerotic stenosis (stenosis rate was more than 50%) scheduled for CEA in Department of Neurosurgery of Affiliated Hospital of Inner Mongolia Medical University from March 2013 to January 2015 were selected. Carotid ultrasound was performed before operation, during operation and after operation for 6-8 hours and 7-10 days. Two dimensional images showed the diameter of the tube, the thickness of the inner membrane, the size of the plaque and the echo. The color Doppler showed the blood flow and the blood flow characteristics of the stenosis, and the changes of hemodynamics were detected by the frequency spectrum of Doppler. Computed tomographic angiography (CTA) was performed before surgery and 7-10 days after surgery. Results The coincidence rate of ultrasound diagnosis and surgical results of carotid artery stenosis was 88.23%. There was high consistency between ultrasound assessment of plaque echo characters and surgical results. The flow rates of carotid arteries stenosis in 51 cases were decreased significantly during operation and after operation, which showed statistically significant differences compared with that pre-operation (P ＜ 0.05); the RI value was increased, with significant difference (P ＜ 0.05); after operation, among 51 vessels, the lumen of 49 vessels was broadened, the blood flew was smooth, and 2 vessels had thrombosis. Ultrasound showed greater consistency to CTA in carotid artery stenosis, the coincidence rate was 76.48%; the plaque echo property evaluated by ultrasound was better than that of CTA. Conclusion Before operation, vascular ultrasound can helps to diagnose carotid stenosis degree accurately, and knows about the echo characteristics and stability of the plaque, intraoperative monitoring for carotid artery can reduce complications during operation; postoperative re-examination can evaluate the surgery effects, decrease the incidence of perioperative stroke and restenosis as well as disability rate and mortality, which has important significance to increase the success rate of CEA. Therefore, intravascular ultrasound can be as a priority for preoperative screening, intraoperative monitoring and postoperative follow-up of CEA.
2017 Vol. 14 (22): 117-120 [Abstract] ( 182 ) HTML (1 KB)  PDF (459 KB)  ( 81 )
121 Comparison of the consistency of blood ALT test results inside and outside station and analysis of the cause of the error
ZHAO Jianpeng1 YANG Xiaoya2▲ YANG Xiuping3 XU Yunpeng1 LI Lihong4
Objective To compare the detection results of alanine aminotransferase (ALT) test of blood donors inside and outside the station, in order to develop corresponding measures to ensure the consistency of the results of different instruments and equipments. Methods The same donors′ blood ALT were measured by automatic biochemical analyzer Hitachi 7100 and semi-automatic biochemical analyzer Photometer 4040 respectively. The results obtained by the Hitachi 7100 were taken as standard results X, and the results of Photometer 4040 were takend as unevaluated results Y (the results of Photometer 4040 on the donation car were Y1; the results of Photometer 4040 in the station were Y2). The dn was calculated by Yn subtracting X, the differences between d1 and d2 were compared, and line charts of dn were made to directly reflect differences in ALT testing results outside and inside the station. The factors leading to different results were analyzed. Results Difference value d1 of Hitachi 7100 and outside blood station 4040 test results was (5.07±4.33), difference value d2 of Hitachi 7100 and inside blood station 4040 test results was (2.40±1.39), the difference was significant (t=7.513, P < 0.05). The fluctuation range of the d1 line chart was obviously higher than that of d2 line chart. Conclusion The instability of the ALT results obtained by semiautomatic biochemical analyzer outside the station is significantly higher than that inside the station. There are many reasons for this result, such as environmental factors of experimental operation, the stability of electric current and voltage of instrument and hemolysis as well as other blood problems in blood collection process.
2017 Vol. 14 (22): 121-124 [Abstract] ( 177 ) HTML (1 KB)  PDF (653 KB)  ( 100 )
125 The effect of innovative teaching-learning model combined with web and classroom teaching in the teaching quality of radiation medicine
WANG Aiping LI Rong SHI Chunmeng XU Hui SU Yongping CHENG Tianmin
Objective To explore effects of innovative teaching-learning model combined with web and classroom teaching on improving the teaching quality of radiation medicine teaching. Methods Traditional teaching method and innovative teaching-learning model incorporating web and classroom teaching were respectively applied to control classes and experimental classes for radiation medicine learning in the years of 2011 and 2012. The classes were randomly constituted by students in clinical medicine major from Third Military Medical University. Questionnaire and grade statistics were used to analyze teaching effect. Results Compared with that in control classes, the percentages of students with better autonomic learning, better understanding ability and much conscious harvest in knowledge, emotion and value orientation in experimental classes were significantly high (P < 0.05). Also, the experimental-class students′ scores including testing-ability question score and written examination score were obviously higher than those of control-class students (P < 0.05). Conclusion The innovative teaching-learning model combined with web and classroom teaching can effectively culture autonomic-learning habit, enhance understanding capacity, and improve the teaching quality of radiation medicine teaching.
2017 Vol. 14 (22): 125-128 [Abstract] ( 163 ) HTML (1 KB)  PDF (678 KB)  ( 99 )
129 Construction of the evaluation index of quality control for standardized training of residents and its implementation
SHI Jingfen1，2 LI Xuexia3
National Health and Family Planning Commission of PRC and other six bureaus dispatched to establish the standardized residents training system in 2013. Then it was put into effect nationwide in 2014. This paper sums up a set of quality assessment index system through literature review and expert consultation and combined with practical work, to explore the evaluation index of improving the quality of standardized residents training. The system includes five dimensions of management, teacher and trainee three levels: basic condition, management system, supporting measure, teacher building and training effect. The practical application shows that the evaluation system is applicable to the current status of standardized residents training in China. It is conducive to promote the quality of training.
2017 Vol. 14 (22): 129-132 [Abstract] ( 175 ) HTML (1 KB)  PDF (672 KB)  ( 97 )
133 The effect of experiential teaching combined with case teaching in the teaching of critical care nursing students
ZHANG Xiumin DING Liumin
Objective To research the effect of experiential teaching combined with case teaching method on independent learning ability and teaching effect of nursing students. Methods From January to December in 2015, 56 nursing students in the Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur Autonomous Region were selected as the control group. From January to December in 2016, 54 nursing students in the Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur Autonomous Region were selected as the experimental group. Before and after the training, the nursing students′self learning ability scale and the related knowledge of the self made critical care medicine were investigated. At the same time, after the end of the training, the teaching effects were evaluated. The independent learning ability, knowledge related to intensive care medicine and teaching quality in the two groups were compared. Results The total score of autonomous learning ability, the scores of knowledge related to intensive care medicine and teaching effect in the experimental group were higher than those in the control group, with statistically significant difference (P < 0.05). Conclusion Experiential teaching combined with case teaching can effectively improve the independent learning ability and knowledge level of nursing students, enhance their learning interest and enthusiasm, and improve teaching quality.
2017 Vol. 14 (22): 133-136 [Abstract] ( 186 ) HTML (1 KB)  PDF (677 KB)  ( 65 )
137 Research of effective form and long-term mechanism of improvement of medical students' ideological education quality
YANG Chongfei1 YAO Chi2 LU Kai2 LI Mingkai3 YAO Jianya2▲
"Health related, life entrusted" is an oath of medical students and the most important mission they undertook. The ideological education of medical students is concerned of the prolonged quality of medical and health services in our nation. Nowadays, new media what blogs, wechat and internet represented become major ways of ideological education in medical college. Changing the model of thinking, harmonizing new and traditional education approaches, enhancing the research of effective form and long-term mechanism, innovating new education platform, strengthening the business ability and team construction of education workers, and helping medical students resisted lure have become issues that worth educators thinking about.
2017 Vol. 14 (22): 137-140,153 [Abstract] ( 702 ) HTML (1 KB)  PDF (700 KB)  ( 138 )
141 Searching of wechat application in clinical postgraduate training of Obstetrics and Gynecology
ZHOU Min HONG Li HU Ming HE Songming HUANG Guotao LU Danhua LI Suting WANG Lingling
Clinical postgraduate students of Obstetrics and Gynecology are the main force of the doctors in clinical Obstetrics and Gynecology Department in the future. With the expansion of graduate education scale, it is important for every medical university to train Obstetrics and Gynecology postgraduates with quantity and quality at the same time. The traditional rigid education mode is unable to meet the current requirements of the clinical postgraduate students cultivation. Wechat platform will be better assist modern teaching mode. This paper discusses wechat application in practice skills and scientific research of clinical postgraduate training of Obstetrics and Gynecology, analyzes the new educational ways of postgraduate training, in order to effectively improve the clinical ability, research capability and the teaching quality of Obstetrics and Gynecology for clinical postgraduates.
2017 Vol. 14 (22): 141-144 [Abstract] ( 179 ) HTML (1 KB)  PDF (624 KB)  ( 139 )
145 Construction of clinical teaching mode of emergency specialist nurses and teaching quality evaluation
YANG Mingli
Objective To explore the construction method of clinical teaching model of emergency first aid nurses, and evaluate the teaching effect. Methods From June to December 2016, 38 specialist nurses participated in teaching training in Department of Emergency, Songgang People′s Hospital of Bao′an District in Shenzhen City were selected as research objects. Ladder training program combined of theory and practice was used. Self-investigation questionnaire was used to evaluate the qualities of the theory teaching and the practical base teaching. The specialized subject theory, the operation skill and the comprehensive practice ability before and after the teaching were evaluated. Results The scores of the two parts of the teaching quality and the clinical practical base teaching were more than 6.6 points (P < 0.05). From the evaluation of nursing ability indexes, after training the students' professional skills, professional knowledge and comprehensive practice ability scores were significantly higher than those before training, and the differences were statistically significant (P < 0.05). Conclusion Application of ladder training program in the clinical teaching mode of emergency specialist nurses, and combination of theory and practice can effectively promote the mastery of theoretical knowledge and the proficiency of practical skills, and improve the clinical practical ability of emergency nurses. It is worthy of clinical application.
2017 Vol. 14 (22): 145-148 [Abstract] ( 165 ) HTML (1 KB)  PDF (680 KB)  ( 94 )
149 Application of GasMan software simulation teaching method in the teaching of inhalation anesthesia
KE Jianjuan CHEN Chao WU Yun ZHANG Zongze
Objective To explore the application value of GasMan computer-based simulation (CBS) teaching method in the teaching of inhalation anesthesia. Methods 100 clinical medicine undergraduates in the Second Clinical Hospital of Wuhan University ("our school" for short) from September to December 2016 were included. They were randomly divided into the control group (group C) and the experiment group (group T), each group had 50 subjects. Group C was given lecture based learning (LBL) teaching method in both theoretical lessons and practical lessons, while group T was given LBL teaching method in theoretical lessons and CBS teaching method in practical lessons. Each teaching method was scored by experts in the class (expert ratings). After the end of the class, the study effect and the satisfaction degree of the two groups of students were investigated by filled teaching effect evaluation (student ratings) and questionnaire. After the course ends, all students took examination and the results were compared between groups to evaluate the teaching effect. Results The expert ratings and student ratings of theoretical lessons did not differ significantly between the two groups (P > 0.05)． However，the expert ratings and student ratings of practical lessons of group T were significantly higher than those of group C (P < 0.05). Group T also got higher satisfaction degrees in questionnaires of learning interests, study efficiency, memorize, logical thoughts, ability of analysis and solving problems and understand ability than those of group C (P < 0.05). Compared to group C, the group T had a higher average ranks (P ＜ 0.05). Conclusion Compared to LBL teaching method, the GasMan CBS teaching method can effectively stimulate learning enthusiasm of students, increase learning initiative, activate classroom atmosphere and improve the teaching effect.
2017 Vol. 14 (22): 149-153 [Abstract] ( 176 ) HTML (1 KB)  PDF (699 KB)  ( 130 )
154 Study and practice of the hospital logistics standard management
ZHANG Jing ZHAO Bin▲
Hospital logistics standard management (HLSM) is a significant part of the hospital management. HLSM is closely related to the construction and development of the hospital, and it is also the important embodiment for the hospital economic and social function degree. Therefore, it is a core issue that enhance HLSM in the hospital modernization management. The characteristics of the hospital logistic management and the connotation, the significance, and the contents of the standard management are analyzed. Together with the experience of HLSM for Beijing Jishuitan Hospital, including establishment of the single-stop-type logistics service center, supervision of the social property management company, device management, human resource management, and satisfaction evaluation, the significance of the standard management in the hospital logistics management are determined. 
2017 Vol. 14 (22): 154-157 [Abstract] ( 129 ) HTML (1 KB)  PDF (443 KB)  ( 67 )
158 Analysis of economic operation of public hospitals based on accounting
LIU Xiaoqi LIU Lihua
The economic operation of public hospitals is often overlooked because of their public welfare. Based on the analysis of the characteristics of public hospitals, this paper analyzes the principles that must be adhered to in the operation of public hospitals and the current status of public hospitals. Due to the characteristics of universality, equality and nonprofit public hospitals themselves, leading to the current management system of public hospitals is not perfect, can not realize the normal operation of the funds without dependence on external capital inflows circumstances. This paper discusses the current public hospitals operating under the background, through the analysis of framework architecture of economic operation of public hospitals, to build a long-term independent development, and do not change the operation system of public welfare nature of public hospitals. The operational analysis framework of the project mainly includes the evaluation framework of hospital's basic situation and benefits, debt and operating capacity, profitability and growth ability of the three aspects, realizing the scientific evaluation results as a basis for the hospital the next strategy improved. This paper also analyzes the function of accounting to the general management of hospital, and changes the status of accounting responsibility only in public hospital accounting. Finally, from the accounting point of view, the economic operation of public hospitals is suggested.
2017 Vol. 14 (22): 158-161 [Abstract] ( 146 ) HTML (1 KB)  PDF (415 KB)  ( 74 )
162 Effect of early rehabilitation nursing on the treatment of knee osteoarthritis under arthroscopic microfracture technique
ZHOU Jing E Xiaoqiang
Objective To explore and analyze the effect of early rehabilitation nursing on the treatment of knee osteoarthritis under arthroscopic microfracture technique. Methods 110 patients with knee osteoarthritis under arthroscopic microfracture technique in the First Affiliated Hospital of Harbin Medical University from June 2014 to June 2016 were selected. All the patients divided into control group and observation group according to random number table, with 55 cases in each group. The control group was received routine nursing, while the observation group was received early rehabilitation nursing on the basis of the control group. The nursing satisfaction, the degree of HSS score were followed up for 6 months, and the quality of life scores of the two groups were compared. Results The satisfaction to nursing care of observation group was obviously higher than that of control group, with statistically significant difference (P < 0.05). The HSS knee score of observation group was better than that of control group, with statistically significant difference (P < 0.05). The scores of body pain, physical function, mental health, social function and overall health score of two groups were increased after treatment, and the increase of  observation group after treatment were more remarkable than those of the control group, with statistically significant difference (P < 0.05). Conclusion Early rehabilitation nursing can help to improve the clinical effect of the microfracture technique on knee osteoarthritis, which can relieve pain and improve the quality of life.
2017 Vol. 14 (22): 162-164,172 [Abstract] ( 166 ) HTML (1 KB)  PDF (577 KB)  ( 98 )
165 Application effect of portable venous puncture auxiliary equipment in tumor chemotherapy patients infusion
HU Yujie1 LI Ping1 YE Ling2 WANG Ya′nan1 ZHANG Manli1
Objective To explore the clinical effects of the self-developed portable venous puncture auxiliary equipment in improving the success rate of intravenous chemotherapy, in order to reduce the vascular damage and improve the efficiency of nurses. Methods From September 2016 to April 2017, 89 patients underwent chemotherapy in Department of Medical Oncology, Air Force General Hospital, PLA were selected and divided into experimental group (venous puncture auxiliary equipment used, 45 cases) and control group (routine puncture, 44 cases). The success rate of one-time puncture, the time of single vein passage, the rate of drug extravasation and phlebitis were compared between the two groups. Results The successful rate of one-time puncture in the experimental group was significantly higher than that in the control group, with statistically significant difference (P < 0.05). The time of establishing a single vein route in the experimental group was significantly less than that in the control group with statistically significant difference (P < 0.05). The incidence of drug extravasation and phlebitis in the experimental group were significantly lower than those in the control group, with statistically significant difference (P < 0.05). Conclusion The portable intravenous puncture auxiliary equipment has the advantages of simple operation and strong practicability, can clearly show the chemotherapy of patients with vascular to help to improve the success rate of vein puncture nurses, reduce the vascular injury caused by repeated puncture of the patients, which is of great significance to protect vessel safety in patients undergoing chemotherapy.
2017 Vol. 14 (22): 165-168 [Abstract] ( 150 ) HTML (1 KB)  PDF (634 KB)  ( 107 )
169 Influence of cognitive behaviors intervention on long-term effects of hepatitis B virus mother to infant transmission-interrupted in patients with middle and late pregnancy
TIAN Hongwei ZHANG Kun SUN Lijuan XU Jianping LI Suwen HOU Junliang
Objective To observe the influence of intervention based on cognitive behaviors on long-term effects of hepatitis B virus mother to infant transmission-interrupted in patients with middle and late pregnancy. Methods 124 patients confirmed hepatitis B virus carried in the middle and late pregnancy in the Fifth Hospital of Shijiazhuang from March 2014 to March 2016 were selected and divided into observation group and control group according to random number table, with 62 cases in each group. The control group was received routine intervention, and the observation group was received intervention based on cognitive behaviors. Psychological status, knowledge of health education, compliance behaviors and neonatal HBsAg positive rate were observed in the two groups. Results After intervention, the scores of SAS and SDS in the two groups were significantly lower than those before intervention (P < 0.05), and those in the observation group were significantly lower than those in the control group (P < 0.05). Knowledge of disease, disinfection and isolation, health care during pregnancy, breastfeeding scores were significantly higher than those before intervention (P < 0.05), which of the observation group were significantly higher than those of the control group (P < 0.05). Regular medication, reasonable diet, appropriate exercise scores of the observation group were significantly higher than those of the control group (P < 0.05). The positive rates of HBsAg at birth, 6 month, and 1 year in the observation group were significantly lower than those in the control group (P < 0.05). Conclusion Intervention based on cognitive behaviors can effectively improve the mastery of health knowledge and compliance behaviors in the middle and later period of pregnancy, and reduce the positive rate of HBsAg in neonates.
2017 Vol. 14 (22): 169-172 [Abstract] ( 154 ) HTML (1 KB)  PDF (563 KB)  ( 99 )
173 Application and effects of individualized iontophoresis nursing scheme on knee osteoarthritis
WANG Haiyan1 YANG Jianying2▲ FU Haiying1
Objective To observe the effect of individualized iontophoresis nursing scheme in application on knee osteoarthritis. Methods It was a randomized parallel control study. One hundred patents with osteoarthritis hospitalized in Orthopedics Ward of Eye Hospital China Academy of Chinese Medical Sciences from January 2015 to June 2016 were selected and divided into control group and observation group by random number table, with 50 cases in each group. All patients were treated by combined therapeutic plan including iontophoresis with herbal decoction. Patients in observation group were treated by individualized nursing scheme which used dynamically adjusted electric current and temperature of decoction in therapy. While participants in control group were managed by conventional nursing scheme with settled current and temperature. Adverse events were recorded to evaluate the safety of two nursing plans. The WOMAC score was collected after therapy as parameter for effect. Results For comparison of WOMAC score within group, patients in both groups were improved in all three subitems (P < 0.05). For comparison of WOMAC between groups, variation of pain and stiffness in observation group were more significant than control group (P < 0.05), while change of physical function was similar in two groups (P > 0.05). For adverse events, incidence rate of blister was not statistically different between two groups (P > 0.05). While incidence rates of erythema and abnormal sensation in observation group were significantly lower than that in control group (P < 0.05). Conclusion In contrast to conventional nursing scheme, the individualized iontophoresis nursing scheme improved the short-term effects on patients with knee osteoarthritis and decrease the adverse events.
2017 Vol. 14 (22): 173-176 [Abstract] ( 170 ) HTML (1 KB)  PDF (595 KB)  ( 82 )
177 Psychological nursing guided by Maslow′s hierarchy of needs improves the high quality nursing for patients with fungal keratitis
LUO Qingyue1 GUO Tingting2▲
Objective To explore the effect of psychological nursing guided by Maslow′s hierarchy of needs theory on the high quality nursing care for patients with fungal keratitis. Methods One hundred patients with (138 eyes) consecutive fungal keratitis admitted in the First Affiliated Hospital of Chongqing Medical University from November 2012 to May 2015 were divided into two groups by convenient sampling method, including 50 cases (66 eyes) in control group and 50 cases (72 eyes) in experimental group. The control group was received routine basic nursing and ophthalmic nursing, while the experimental groups was received psychological nursing intervention guided by Maslow′s hierarchy of needs based on the control group. Then, the Self-assessment Lists of Symptom (SCL-90) scores in the two groups on admission, during the therapeutic and at the time of discharge were compared. The quality nursing satisfaction scores were compared between the two groups at the time of discharge. Results At the time of admission, all factors evaluated in the SCL-90 in the both groups were higher than the normal value (P ＜ 0.05). There were no differences between the two groups (P > 0.05). During the therapeutic and at the time of discharge, the SCL-90 scores of the two groups were increased significantly compared with those at the time of admission (P ＜ 0.05). The scores of somatization factor, interpersonal sensitivity factor, depression factor and anxiety factor in the experimental group were significantly lower than those in the control group (P < 0.05). At the time of discharge, the SCL-90 scores for interpersonal sensitivity factor, depression factor, anxiety factor, hostility and phobia in the experimental group were significantly lower than those in the control group (P < 0.05). The quality nursing satisfaction scores in the experimental group were significantly higher than that in the control group (P < 0.05). Conclusion Psychological nursing guided by Maslow′s hierarchy of needs theory can effectively improve psychological state of the fungal keratitis patients, and improve the quality of nursing satisfaction.
2017 Vol. 14 (22): 177-180 [Abstract] ( 160 ) HTML (1 KB)  PDF (598 KB)  ( 83 )
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