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| Effect of Shujin Jianyao Pills on ischiadic nerve and cytokine in serum of CCI rats |
| SHI Hongchao1 OU Huiyu2 HE Fenglei1 CHEN Dixin2 PENG Fuquan1 LI Ruipeng2 MO Guoqiang1 |
1.Guangzhou Baiyunshan Chenliji Pharmaceutical Factoty Co., Ltd., Guangdong Province, Guangzhou 510288, China;
2.Drug Non-Clinical Evaluation and Research Center of Guangzhou Pharmacutical Research Nstitute, Guangdong Province, Guangzhou 510240, China |
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Abstract Objective To study the effect of Shujin Jianyao Pills on the pathological changes of ischiadic nerve and cytokine in the serum of CCI (chronic constriction injury) rats. Methods 112 SD rats were randomly divided into sham operation group, model group, Zhuanggu Guanjie Pill group, Paracetamol and Caffeine Tablets group, Shujin Jianyao Pill low dose group (1.10 g/kg), middle dose group (2.19 g/kg) and high dose group (4.38 g/kg), with 16 rats in each group. The right common sciatic nerve was exposed by blunt dissection, and four ligatures were tied loosely around the sciatic nerve. Sham operation referred to the procedure of exposing the nerve as above but without any nerve ligation. All groups were administrated with corresponding drugs by intragastric administration once a day for 28 days. After the last administration, the hindpaw withdrawal latency (HWL) of the rats, the contents of inflammatory cytokines including TNF-α, IL-1β, IL-6, PGE2 in the serum and the pathological changes of ischiadic nerve were detected. Results Compared to the sham operation group, in the model group, the HWL was shortened, ischiadic nerve was become swollen and vacuolar, the serum levels of IL-1β and IL-6 were increased (P < 0.05). Compared to the model group, in the Shujin Jianyao Pill groups, the HWL were extended, the pathological changes were ameliorated induced by CCI, and the IL-1β and IL-6 levels were decreased (P < 0.05). Conclusion Shujin Jianyao Pill can alleviate the histologic changes of sciatic nerve, inhibit the expression of inflammatory cytokines, which has therapeutic effect on traumatic neuralgia.
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