人脐带间充质干细胞经动脉介入治疗2型糖尿病Beagle犬的作用

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Abstract Objective To investigate the therapeutic effects of human umbilical cord mesenchymal stem cells (UCMSCs) transplanted into a dog model of type 2 diabetes mellitus (T2DM) via the arterial intervention. Methods 24 Beagle dogs were randomly divided into UCMSCs treatment group (treatment group, n = 11), diabetic model control group (model group, n = 8) and normal control group (normal group, n = 5). Dogs in treatment and model control group were fed on high fat diet and intravenously injected with alloxan to establish T2DM models. Treatment group accepted insulin therapy and UCMSCs transplantation via arterial intervention. Model group only received insulin therapy, and normal group did not receive any treatment. Distribution of UCMSCs traced by CM-Dil was observed under fluorescence microscope. In addition, the three groups were compared with body mass, blood fat, fasting plasma glucose, insulin dosage, Area under the curve of C-peptide, insulin secretion index (HOMA-β), insulin resistance index (HOMA-IR) before and after therapy. Results The cells traced by CM-Dil mainly distributed in the pancreas and kidney. At 4 weeks after transplantation, fasting plasma glucose and insulin dosage were decreased gradually in treatment group , up to 12 weeks average daily insulin dose was declined from (27.94±1.86) U to (15.02±2.90) U. Compared with the model group, at 12 weeks triglyceride, low density lipoprotein cholesterol, HOMA-IR decreased (P < 0.05), under the curve of C-peptide, HOMA-β increased in treatment group (P < 0.05). Conclusion The method of translation of UCMSCs by artery intervention not only maximum guarantee the stem cells quantity into the pancreas , but also has great effects on reversing glucose and lipid metabolism disorder, recovering islet β cell function, decreasing insulin dosage, and relieving insulin resistance.

Key words： Umbilical cord mesenchymal stem cells Arterial intervention Type 2 diabetes mellitus Islet function Insulin resistance

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	DONG Song WANG Hong LEI Lei SHI Han WANG Yizhong

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DONG Song WANG Hong LEI Lei SHI Han WANG Yizhong. Action of human umbilical cord mesenchymal stem cells by intra-arterial transplantation for treatment of type 2 diabetic Beagle dogs [J]. 中国医药导报, 2017, 14(22): 16-20.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I22/16

[1] 王琳琳，崔晓兰，时瀚，等.人脐带间充质干细胞联合番茄红素延缓比格犬的自然衰老[J].中国组织工程研究，2014，18（45）：7239-7245.
[2] De Schauwer C，Goossens K，Piepers S，et al. Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources [J]. Stem Cell Res Ther，2014，5（1）：6.
[3] Tsai PJ，Wang HS，Shyr YM，et al. Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin induced diabetic rats [J]. J Biomed Sci，2012，19（1）：1-9.
[4] 柴树宏，鲍艳春.人脐带间充质干细胞与骨髓间充质干细胞分化为胰岛素分泌细胞的能力比较[J].实用医学杂志，2014，30（1）：52-54.
[5] 张迪，翁孝刚，王涛，等.不同途径移植人脐带间充质干细胞治疗糖尿病大鼠[J].新乡医学院学报，2013，30（5）：347-352.
[6] 李青，谢洪斌，王云帅，等.不同移植途径对人脐带间充质干细胞防治NOD鼠糖尿病的影响[J].免疫学杂志，2012， 28（5）：398-402.
[7] 王丽梅，崔晓兰，丁明超，等.自体骨髓间充质干细胞经动脉介入移植治疗犬糖尿病[J].中国组织工程研究，2012， 16（19）：3545-3550.
[8] Kadam SS，Bhonde RR. Islet neogenesis from the constitutively nestin expressing human umbilical cord matrix derived mesenchymal stem cells [J]. Islets，2010，2（2）：112-120.
[9] 禹亚斌，陈维，卞建民.体外定向诱导人脐带间充质干细胞分化为胰岛样细胞[J].中国组织工程研究，2013，17（6）：1012-1017.
[10] 申义，王意忠，时瀚，等.人脐带间充质干细胞分化为胰岛样细胞过程中胰岛素和巢蛋白的表达[J].中国组织工程研究，2016，20（50）：7500-7506.
[11] 王英，罗浩红，高洪泉，等.体外培养人脐带间充质干细胞向胰岛细胞分化及对糖尿病治疗的实验研究[J].中国实用医学，2014，9（14）：1-3.
[12] Hu J，Wang F，Sun R，et al. Effect of combined therapy of human Wharton's jelly-derived mesenchymal stem cells from umbilical cord with sitagliptin in type 2 diabetic rats [J]. Endocrine，2013，45（2）：279-287.
[13] Thorel F，Nepote V，Avril I，et al. Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss [J]. Nature，2010，464（7292）：1149-1154.
[14] Hasegawa Y，Ogihara T，Yamada T，et al. Bone marrow（BM） transplantation promotes β-cell regeneration after acute injury through BM cell mobilization [J]. Endocrinology，2007，148（5）：2006-2015.
[15] Xu YX，Chen L，Hou WK，et al. Mesenchymal stem cells treated with rat pancreatic extract secrete cytokines that improve the glycol-metabolism of diabetic rats [J]. Transplant Proc，2009，41（5）：1878-1884.
[16] Urban VS，Kiss J，Kovacs J，et al. Mesenchymal stem cells cooperate with bone marrow cells in therapy of diabetes [J]. Stem Cells，2008，26（1）：244-253.
[17] Manochantr S，U-pratya Y，Kheolamai P，et al. Immunosuppressive properties of mesenchymal stromal cells derived from amnion，placenta，Wharton′s jelly and umbilical cord [J]. Intern Med J，2013，43（4）：430-439.
[18] Chhabra P，Brayman KL. Stem cell therapy to cure type 1diabetes：from hype to hope [J]. Stem Cells Transl Med，2013，2（5）：328-336.
[19] Si Y，Zhao Y，Hao H，et al. Infusion of mesenchymal stem cells ameliorates hyperglycemia in type 2 diabetic rats： identification of a novel role in improving insulin sensitivity [J]. Diabetes，2012，61（6）：1616-1625.
[20] 高舒平，曲春辉，周瑞，等.干细胞与转化医学研究进展浅析[J].中国实验动物学报，2016，24（4）：439-442.
[21] 李丽，赵博.脐带间充质干细胞移植治疗大鼠糖尿病[J].中国组织工程研究，2014，18（19）：3094-3099.