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| Metabolomic study of protective effect of aucubin for photoaging ESF-1 cells induced by UVA |
| LI Xue1 LEI Xuexue1 XU Hongdan2▲ ZHOU Jing1▲ |
1.The First Hospital of Jilin University, Jilin Province, Changchun 130021, China;
2.Jiamusi College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China |
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Abstract Objective To analyze the mechanism and effective target of aucubin intervene photoaging ESF-1 cells by high through-put cell metabolomics. Methods Cell photoaging model was established by UVA cell injury method, then given low medium and high dose aucubin DMEM medium for the intervention. The activities and contents of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde (MDA) were detected for the evaluation of oxidative damage and aucubin treatment effect. Based on above analysis and high resolution mass spectrometry, the high throughput metabolic footprint analysis was conducted in the normal cells, photoaging ESF-1 cells and aucubin intervention cell. Massive metabolic data were analzyed with dimension reduction process and multivariate statistics by model discriminating technique. Results Compared with the blank group, the activities of SOD, GSH-Px, CAT significantly decreased and contents of MDA significantly increased in the model group (P < 0.01). Compared with the model group, the activities of SOD, GSH-Px, CAT increased and contents of MDA decreased in the medication gorups, of which, in the medium and high doses medication groups the differences were statistically significant (P < 0.05 or P < 0.01). There were significant differences in the metabolism between the normal cells and the photoaging ESF-1 cells, and the disturbance of metabolism was mainly concentrated in arachidonic acid metabolism, glutathione metabolism. 6 core biomarkers were identified by focusing analysis, and these small molecular metabolites were callback after the administration of aucubin. Conclusion Aucubin has a favorable effect on photoaging, and the mechanism may be related to arachidonic acid metabolism. Besides, the present investigation provides new ideas and methods for early prevention and drug development.
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