子宫腔脱落细胞的DNA倍体分析技术应用于子宫内膜病变筛查的研究

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Abstract Objective To investigate the feasibility and application value of DNA quantitative analysis of uterine cavity exfoliated cells in screening endometrial lesions. Methods Five hundred patients with suspected endometrial lesions from January 2014 to May 2016 in the Second Affiliated Hospital of Guiyang Traditional Chinese Medicine College were selected as the research objects, specimens of uterine cavity exfoliated cells were collected, DNA ploidy was analyzed by Feulgen staining and automatic DNA quantitative analysis system, segmented diagnostic curettage was performed at the same time, the segmented diagnostic curettage results were the gold standard, the sensitivity and specificity of DNA quantitative analysis in diagnosis of endometrial lesions was observed. Results Segmental diagnostic curettage detection showed, 39 cases of endometrial carcinoma (78.0%), 98 cases of precancerous lesion (19.6%), 258 cases of endometrial hyperplasia (51.6%), 105 cases of normal endometrium (21.0%). The detection rate of endometrial carcinoma of DNA ploidy analysis of uterine cavity exfoliated cells was similar to pathological examination, the difference was not statistically significant (P > 0.05). DNA ploidy analysis of exfoliated uterine cavity precancerous lesions were similar to the pathological examination, the difference was not statistically significant (P > 0.05). With the pathological biopsy results as the gold standard, the sensitivity, specificity and accuracy of endometrial lesions DNA quantitative analysis were 92.7%, 83.8% and 86.2% respectively. The sensitivity, specificity and accuracy of DNA quantitative analysis in screening endometrial cancer were 94.9%, 67.7% and 69.8% respectively. Conclusion DNA quantitative analysis of endometrial cancer has high screening sensitivity, can be used for early screening of endometrial lesions, has a certain clinical value.

Key words： Uterine cavity exfoliated cells Image cytometry DNA ploidy DNA quantitative analysis Endometrial lesions Screening

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	HUA Zhaozhao ZENG Li CAO Junyan XIONG Wei LUO Deyi ZHANG Xianghua LIU Xuan CHENG Li

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HUA Zhaozhao ZENG Li CAO Junyan XIONG Wei LUO Deyi ZHANG Xianghua LIU Xuan CHENG Li. Research of DNA quantitative analysis of uterine cavity exfoliated cells in endometrial lesions screening[J]. 中国医药导报, 2018, 15(11): 86-90.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I11/86

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