血清中针对TORCH相关病原的IgG和IgM抗体分析

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Abstract Objective To provide theoretical guidelines for reasonable application of TORCH assay in aristogenesis and fine rearing through analysis of serum antibody of IgG and IgM against TORCH-associated pathogens (TOX, RV, CMV, HSV1/2) from the pre-pregnant, pregnant patients at early stage and new-born patients. Methods From January of 2014 to December of 2017, 19 533 serum samples were collected from pre-pregnant (pre-pregnant group, 1929 samples), pregnant patients at early stage (pregnant group, 15 555 samples) and new-born patients (new-born group, 2049 samples) in the First People's Hospital of Kunming. ELISA was performed to detect the levels of antibodies IgG and IgM against TORCH-associated pathogens and further determine positive rates of specific antibodies of IgG and IgM. Results ELISA of TORCH antibodies in 19 533 serum samples showed that the positive rate of antibody IgG against any of TORCH-pathogens reached 99.41%. The positive rate of CMV IgG (97.27%) was the highest among four kinds of TORCH-associated pathogens and TOX (3.7%) showed the lowest rate. The positive rate of IgM antibody was 12.8%. The positive rate of HSV1 (6.44%) was the highest and CMV showed the lowest rate (0.39%). Further analysis of the positive rate of IgG by groups showed the more than 99% of IgG positive rate of TORCH-associated pathogens in all groups of prepregnant group, pregnant group at early stage and new born group. The IgM positive rates of TORCH-associated pathogens in pre-pregnant group and pregnant group at early stage, 18.87% and 13.65%, were higher than that in new-born group (0.63%). There were more than 60% of individual serum sample showed the IgG antibodies against 3 TORCH-associated pathogens. However, most of the IgM antibody in individual serum sample was against one of TORCH-associated pathogens. Conclusion The pre-pregnant, pregnant women at early stage and new-born patients are screened for antibodies of IgG and IgM in serum against TORCH-associated pathogens, which is of instructive significance for controlling infections of TORCH pathogens and aristogenesis and fine rearing.

Key words： TORCH Infection Serum antibody ELISA Aristogenesis and fine rearing

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	YANG Yuelin1
	2 LUO Chun2 HU Dachun3 TANGShurong3 LIU Hongqi1

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YANG Yuelin1,2 LUO Chun2 HU Dachun3 TANGShurong3 LIU Hongqi1. Analysis of antibodies IgG and IgM against TORCH-associated pathogens in serum samples[J]. 中国医药导报, 2018, 15(11): 82-85,103.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I11/82

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