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| Preparation of the macroporous polysaccharide hemostatic complex materials and study on its performance in vitro |
| XU Ling1 MA Xiaochun1 ZENG Lijun2 CHEN Miaoyuan2 WANG Yun2 JU Xuecheng1 QIU Ximei1 |
1.Peking University Shenzhen Institute, Guangdong Province, Shenzhen 518057, China;
2.Xiamen Gelfeel Biological Technology Co., Ltd., Fujian Province, Xiamen 361026, China |
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Abstract Objective To evaluate the hemostatic effect of the macroporous polysaccharide hemostasis complex materials preliminarily, and to investigate the performance of the materials in vitro. Methods The hemostatic materials were prepared by γ-irradiation crosslinked method, and its structure, degradation in vitro and clotting performance were characterized by infrared spectrum and physicochemical test. Results The results showed that the crosslinked carboxymethylated-chitosan molecules had been made after the materials were irradiated, and its water absorption was of 3456%. The biodegradable degradation of the materials in lysozyme solution was 68% after 3 days and the degradation completed within 20 days. The clotting time was (25.33±2.45) s, which indicated the material adhered more red blood cells and platelets and showed the stronger blood clots. Conclusion The macroporous polysaccharide hemostasis complex materials prepared by γ-irradiation crosslinked method possess great solution absorption performance and blood coagulation performance, which has good application prospect in wound hemostasis.
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