丹参酮ⅡA对万古霉素损伤HK-2细胞凋亡的影响及其机制的研究

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Abstract Objective To explore the effect of TanshinoneⅡA Sulfonate Injection against HK-2 cell damage induced by Vancomycin and the probable mechanism. Methods HK-2 cells were cultured in vitro till the number was up to 1×106 /mL, randomly divided into five groups: control group, VAN group, low dose group of TSⅡA Sulfonate Injection, middle dose group of TSⅡA Sulfonate Injection, hight dose group of TSⅡA Sulfonate Injection. Except for the control group adding PBS, other groups were added 100 μg/L VAN for 24 hours. Then, the control group and VAN group were respectively added to the same volume of PBS, and the high dose group, middle dose group and low dose group of TSⅡA respectively added to different concentrations of TSⅡA. The cell viability was detected with MTT method, the activity of LDH were measured and the cell viability was detected with the content of LDH. DCFH-DA was used as the fluorescence probe to detect the level of ROS by a fluorescence microplate reader. Annexin V-FITC/PI double dyeing method was used to detect apoptosis rate. Results Compared with the control group, the survival rate of the cells and the activity of CAT were decreased, meanwhile the level of ROS, the activity of LDH and apoptosis rate were increased in the VAN group (P ＜ 0.05). Compared with the VAN group, the survival rate of the cells and the activity of CAT were increased, meanwhile the level of ROS, the activity of LDH and apoptosis rate were decreased in the low, middle and hight dose group of TSⅡA Sulfonate Injection (P ＜ 0.05), and there was a concentration dependence. Conclusion TSⅡA Sulfonate Injection can promote the proliferation of HK-2 cells in vitro, and improve the biochemical parameters and enzyme levels. The results suggest that TSⅡA Sulfonate Injection has a protective effect on HK-2 cell, and the protective mechanisms may be related with its antioxidant effect.

Key words： Vancomycin TanshinoneⅡA Cell apoptosis Mechanism HK-2 cell

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	XI Jiaxi ZHANG Huajun CHEN Xiaoyu

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XI Jiaxi ZHANG Huajun CHEN Xiaoyu. Effect of TanshinoneⅡA Sulfonate Injection against HK-2 apoptosis induced by Vancomycin and the probable mechanism[J]. 中国医药导报, 2018, 15(11): 8-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I11/8

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