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| Effect of optimized myocardial energy metabolismon on the expression of hypoxic induction factor-1 and vascular endothelial growth factor in rats with superior mesenteric venous thrombosis |
| YU Xiangyang XIE Zongyuan CHEN Jianli ZHANG Guozhi CAO Wenbin DAI Liang HE Yanfang▲ |
| Department of General Surgery, the Affiliated Hospital of North China University of Technology, Hebei Province, Tangshan 063009, China |
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Abstract Objective To study the effect of optimized myocardial energy metabolism on the expression of hypoxic induction factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) in rats with superior mesenteric venous thrombosis. Methods Forty-five healthy adult SD rats were divided into control group, model group and treatment group by random number table. The model group animals were tested for the model, which were fed after subcutaneous injection of 0.2 mL/100 g of physiological saline solution. After the animals were formed, the tail vein was injected with Trimetazidine (10 mg/kg) in the treatment group. The control group was only opened the abdominal cavity, but was not block the blood transport, then injected the physiological saline with 0.2 mL/100 g. Each group was given a 12 h interval for drug intervention until 72 h after surgery. General state, abdominal cavity condition and pathological condition were observed, HIF-1, VEGF and malondialdehyde (MDA), creatine kinase (CK), and adenosine triphosphate (ATP) levels were determined. Results The general situation, peritoneal condition and intestinal histopathological observation of control group were normal, while intestinal of the model group was necrosis, the part region was perforation, consciousness and activity of treatment group, blood transport of intestinal was basically normal, and there was no bleeding edema and no necrosis. Compared with the control group, the levels of HIF-1, VEGF, MDA and CK in the model group were significantly higher, ATP level was decreased, with statistically significant difference (P < 0.05). Compared with the model group, the levels of HIF-1, VEGF, MDA and CK in the treatment group were decreased significantly, ATP level was higher, with statistically significant difference (P < 0.05). Conclusion Optimize energy metabolizer Trimetazidine can remove free radical damage to myocardial cells, and can significantly reduce VEGF and hif-1 levels, improve the condition of venous thrombosis in animal intestines with good effect.
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| Cite this article: |
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YU Xiangyang XIE Zongyuan CHEN Jianli ZHANG Guozhi CAO Wenbin DAI Liang HE Yanfang▲. Effect of optimized myocardial energy metabolismon on the expression of hypoxic induction factor-1 and vascular endothelial growth factor in rats with superior mesenteric venous thrombosis[J]. 中国医药导报, 2018, 15(9): 13-17.
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| http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I9/13 |
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