远程缺血预处理对兔脊髓缺血再灌注损伤的保护作用及其机制

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Abstract Objective To investigate the protective effect of remote ischemic preconditioning (RIPC) on spinal cord ischemia reperfusion injury (SCIRI) and its possible mechanism in a rabbit model. Methods Thirty-six rabbits were randomly divided into sham operation group (Sham group), ischemia reperfusion group (IR group), IR+ RIPC group, 12 rats in each group. On the 2 sec and 5th day after reperfusion, 6 animals were sacrificed respectively. Animals in Sham group were exposed abdominal aorta (without interruption), IR group and IR+RIPC group received abdominal aortic clamp 30 min, and established SCIRI model. IR + RIPC group underwent RIPC before aortic clamp 1 h. All animals were evaluated for hindlimb function scores in postoperative 2 days and 5 days, according to the Tarlov standard, and then were sacrificed and the L3 - L5 segment of the spinal cord were detected to observe the pathological changes of spinal cord tissues. Meanwhile, the SOD1 activity and the expression level of SOD1 mRNA were detected. Results On the same timepoint, compared with the Sham group, the neurological function scores and pathological grading of spinal cord tissue in IR group were significantly decreased (P < 0.01）, but compared with the IR group, the neurological function scores and pathological grading of spinal cord tissue in IR+ RIPC group were significantly improved (P < 0.01). On the second day after operation, compared with the Sham group, the activity of SOD1 and the expression level of SOD1 mRNA in the IR+RIPC group were all significantly increased (P < 0.01), whereas there was no significant change in the IR group (P > 0.05). On the 5th day after operation, compared with the Sham group, the activity of SOD1 and the expression level of SOD1 mRNA in the IR group were all significantly decreased (P < 0.01), and there was no significant change in the IR+RIPC group (P > 0.05). Compared with IR group, the SOD1 activity and the expression level of SOD1 mRNA were all significantly increased in the RIPC group (P < 0.01). The change of SOD1 mRNA expression in spinal cord was positively related to the change of SOD1 activity in IR group or IR+ RIPC group (R=0. 96 or 0.97, all P < 0.01). Conclusion RIPC has a certain preventive effect on rabbit SCIRI, and its mechanism is related to enhancing the expression of SOD1 in ischemic region of spinal cord tissues.

Key words： Remote ischemic preconditioning Spinal cord Ischemia reperfusion injury Superoxide Dismutase-1

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	YU Qijing1 TIAN Wenhua2 YANG Yunzhao1

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YU Qijing1 TIAN Wenhua2 YANG Yunzhao1. Protective effect of remote ischemic preconditioning on spinal cord ischemia reperfusion injury and its possible mechanism in a rabbit model[J]. 中国医药导报, 2018, 15(9): 8-12.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I9/8

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