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| Autophagy of Cajal mesenchymal cells in the colon Cajal in rats with slow transit constipation and the qualitative analysis of calcium ion level |
| TIAN Yu MAO Yu′an▲ |
| Department of Digestive Medicine, the Sixth Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830002, China |
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Abstract Objective To investigate the autophagy of Cajal mesenchymal cells in the colon Cajal in rats with slow transit constipation, and qualitatively analyze the calcium ion level. Methods Forty healthy Wistar male rats were divided into control group (n=20, normal rats) and model group (n=20, model rats) by random number table. Compound phenylethylpiperidine was used for preparation on STC rat model, grain number, every grain of waste quality, body quality of rats, the first grain of black were recorded to judge the model successfully established or not. Colon ICCs of rats in two groups were detected bydetection of immunofluorescence assay, and the concentration of calcium ions in ICCs was determined by immunofluorescence assay. Results The control group had good growth during the experiment, and one died in the model group. Daily waste grain number, average body quality after 30 days in model group were less than those in the control group(P < 0.01), the average grain of feces quality was higher than that in the control group (P < 0.05), and the first row of black time was obviously prolonged in the control group (P < 0.05). The rats colon tissue of ICCs in control group were intertwined to form the intensive and precision of the mesh structure, they had obvious cell branch. The number of ICCs in the intestinal tract of the model group was significantly reduced, and the ICCs was thin and the cell shape narrowed. Compared with the control group, the autophagy number of ICCs in the model group increased and showed the apparent autophagy death. In the control group, ICCs were smooth and had multiple directional and irregular cell branches, and intercellular interaction was connected to the network structure. The number of fluorescence spots and fluorescence intensity in the ICCs of STC rats were significantly higher than those in the control group. Conclusion The number of ICCs in STC rats is reduced and autophagy is occurred. In the STC rat model, the free Ca2+ ions in the ICCs cells are elevated, and the increasing of Ca2+ ion may be the cause of the ICCs′ autophagy death.
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