石仙桃多糖对肺炎支原体感染模型小鼠Th1/Th2细胞因子表达的影响

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Abstract Objective To investigate the effects of polysaccharide of Pholidota chinensis Lindl. on the expression of γ- interferon (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-12 and other cytokines in bronchoalveolar lavage fluid (BALF) of model mice of Mycoplasma pneumoniae pneumonia. Methods By matched pair design random method, ninety BALB/c nude mice were randomly divided into three groups: model group, Clarithromycin group and polysaccharide of Pholidota chinensis Lindl. group, with 30 mice in each group. PPLO broth medium was used to cultivate mycoplasma pneumonia, the successfully cultivated Mycoplasma pneumoniae was dropped into the nasal cavity of nude mice 1×107 CCU/mL bacteria solution, each 50 μL (1, 5, 8 d). From the ninth day of modeling, polysaccharide of Pholidota chinensis Lindl. group was started to be use polysaccharide of Pholidota chinensis Lindl., Clarithromycin group was given 15 mg/kg of Clarithromycin by intragastric administration, continued for 6 d, the model group was given same volume of normal saline by gavage. After intervention for 1, 3, 6 d, the mice BALF was collected, SP4 agar plate was used to cultivate BALF Mycoplasma pneumoniae quantificationally, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-1β, IL-12, IFN-γ, TNF-α. Lung tissue was fixed with neutral formalin to take HE staining. The pathological degree of mice infected by Mycoplasma pneumoniae was observed. Results Compared with model group, the number of bacterial colonies of Mycoplasma pneumoniae cultivated by BALF quantificationally in polysaccharide of Pholidota chinensis Lindl. group was reduced significantly (P ＜ 0.05), the pulmonary inflammation was improved significantly, and the scores of lung pathology and pulmonary parenchyma were decreased significantly (P ＜ 0.05 or P ＜ 0.01). After treatment for 1, 3, 6 d, the levels of IL-1β, IL-12, IFN-γ, TNF-α in polysaccharide of Pholidota chinensis Lindl. group were significantly lower than those of model group, the differences were statistically significant (P ＜ 0.05). Conclusion Polysaccharide of Pholidota chinensis Lindl. can significantly reduce the pulmonary inflammation of mice with Mycoplasma pneumoniae pneumonia, the mechanism may be related to reduce the expression of Th1/Th2 cytokines of IL-1β, IL-12, IFN-γ, TNF-α in model mice with Mycoplasma pneumoniae pneumonia.
[Key words] Pholidota chinensis Lindl.; Polysaccharide; Mycoplasma pneumoniae; Cytokine; Th1/Th2

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	HUANG Meiqing1 ZHU Yanhua2 ZHAO Shiyuan2

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HUANG Meiqing1 ZHU Yanhua2 ZHAO Shiyuan2. Effect of polysaccharide of Pholidota chinensis Lindl. on the expression of Th1/Th2 cytokines in mice infected with Mycoplasma pneumoniae[J]. 中国医药导报, 2018, 15(8): 15-19.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I8/15

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