经关节穿刺注射ADSCs修复兔激素性股骨头坏死的研究

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Abstract Objective To investigate the effect of adipose derived stem cells (ADSCs) injection through the joint puncture on the repair of steroid-induced avascular necrosis of femoral head in rabbits. Methods ADSCs were isolated and passaged from the neck adipose tissue of adult New Zealand white rabbits. Thirty-six New Zealand white rabbits were used to establish the model of steroid-induced avascular necrosis of femoral head by horse serum combined with hormone. These rabbits were randomly divided into control group, core decompression group and ADSCs group (12 rabbits in each group), and all for bone density detection. Control group:no intervention; core decompression group: femoral head drilling decompression; ADSCs group:by intra-articular injection of ADSCs. At 4 weeks and 8 weeks after intervention, 6 rabbits in each group were examined with bone mineral density (BMD) and X-ray examination. After the death, their femur samples were collected for gross observation, HE staining and PCR were used for the measurement of BMP-2 mRNA. Through the comparison of each group to explore the effect of ADSCs repairing steroid-induced avascular necrosis of the femoral head by joint puncture injection. Results 4 weeks and 8 weeks after intervention three groups of BMD, but the control group decreased, compared with core decompression group and ADSCs group were statistically significant (P < 0.05), but there was no significant difference between the core decompression group and the ADSCs group (P > 0.05). General observation, imaging and histological findings at 4 weeks and 8 weeks after intervention, compared with the control group, ADSCs treatment by joint puncture injection and core decompression both can delay the progress of femoral head necrosis. There had a repair effect on femoral head necrosis, and the effects in ADSCs group were better than those in core decompression group; at 4 weeks and 8 weeks after intervention, the expression of BMP-2 mRNA in the ADSCs group and the core decompression group were both higher than those in the control group, the differences were statistically significant (P < 0.05), while there was no significant difference between the core decompression group and the ADSCs group (P > 0.05). Conclusion ADSCs injected through the joint puncture can repair the steroid induced avascular necrosis of the femoral head in rabbits.

Key words： [Avascular necrosis of the femoral head Adipose-derived stem cells Joint puncture Repair

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	TAN Weiyuan AN Rongze QI Xinwen XIE Jiaqing

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TAN Weiyuan AN Rongze QI Xinwen XIE Jiaqing. Repair of rabbit steroid-induced avascular necrosis of femoral head by ADSCs injection through the joint puncture[J]. 中国医药导报, 2018, 15(6): 175-180.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I6/175

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