乳腺浸润性小叶癌组织中miR-211-5p、SETBP1的表达及其意义

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Abstract Objective To detect the expression of miR-211-5p and SET binding protein 1 (SETBP1) in the tissue of infiltrating lobular carcinoma (ILC) of the breast, and to explore the clinical significance of their expression in ILC. Methods The clinicopathological data of 82 female patients with ILC treated in the First Affiliated Hospital of Hainan Medical University from January 2019 to September 2020 were collected. Fluorescence quantitative PCR was used to detect the expression levels of miR-211-5p and SETBP1 in cancer tissues and adjacent tissues. The relationship between the expression of miR-211-5p and SETBP1 and the clinicopathological features of ILC patients was analyzed. Pearson linear correlation was used to analyze the correlation between miR-211-5p and SETBP1 expression in ILC. Bioinformatics software was used to predict the binding sites of miR-211-5p and SETBP1. Results The expression of miR-211-5p in ILC was lower than that in adjacent tissues, and the difference was statistically significant (P ＜ 0.05). The expression of SETBP1 in ILC was higher than that in adjacent tissues, and the difference was statistically significant (P ＜ 0.05). Compared with tumor TNM stage Ⅰ to Ⅱ ILC cancer tissues, miR-211-5p expression was significantly lower and SETBP1 expression was significantly higher in tumor TNM stage Ⅲ to Ⅳ ILC cancer tissues (P ＜ 0.05). Compared with ILC cancer tissues without axillary metastasis, miR-211-5p expression was significantly lower in ILC cancer tissues with axillary metastasis, while SETBP1 expression was significantly higher (P ＜ 0.05). The expression of miR-211-5p was negatively correlated with SETBP1 in ILC carcinoma tissues (r = -0.573, P < 0.05). Bioinformatics software predicted the existence of potential complementary binding sites with miR-211-5p at bases 374 to 381 in the 3’UTR region of SETBP1. Conclusion The expression of miR-211-5p is decreased in ILC cancer tissues, and SETBP1 is increased in ILC cancer tissues. The expressions of miR-211-5p and SETBP1 are correlated with tumor stage and axillary lymph node metastasis in breast cancer patients, and they are both involved in the progression of ILC, which may be diagnostic and therapeutic targets of ILC.

Key words： Infiltrating lobular carcinoma of the breast miR-211-5p SET binding protein 1 Clinicopathological parameters

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	WENG Xiangqian1 WANG Yeling1 LIN Bing1 ZHANG Shuang1 SU Jun2

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WENG Xiangqian1 WANG Yeling1 LIN Bing1 ZHANG Shuang1 SU Jun2. Expression and significance of miR-211-5p and SETBP1 in the tissue of infiltrating lobular carcinoma of the breast#br#[J]. 中国医药导报, 2021, 18(32): 108-112.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I32/108

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