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| Effect of Shenxiong Glucose Injection on permeability of blood-cerebrospinal fluid barrier in hypoperfusion rats#br# |
| FENG Peipei ZHANG Qianying HUANG Yan LI Yongjie LIU Kun LI Min |
| Department of Pharmacy, North China University of Sience and Technology Affiliated Hospital, Hebei Province, Tangshan 063000, China |
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Abstract Objective To study the effect of Shenxiong Glucose Injection on the permeability of blood-cerebrospinal fluid barrier in hypoperfusion rats. Methods Sixty SPF male SD rats were divided into sham operation group, hypoperfusion group and Shenxiong group by random number table method, with 20 rats in each group. In the hypoperfusion group and Shenxiong group, the common carotid artery was ligation to make an animal model of cerebral ischemia, while in the sham operation group, only the common carotid artery was exposed without ligation. Shenxiong group was intraperitoneally injected with Shenxiong Glucose Injection 24 mg/kg, sham operation group and hypoperfusion group were intraperitoneally injected with normal saline. Serum albumin (SAlb) and cerebrospinal fluid albumin (CAlb) were determined by enzyme linked immunosorbent assay. The mRNA of ZO-1 and Occludin in choroid plexus was detected by real-time fluorescence quantitative PCR. The fluorescence intensity of ZO-1 and Occludin in choroid plexus was detected by laser confocal method. Results CAlb and serum albumin and cerebrospinal fluid quotient (QAlb) in the hypoperfusion group were higher than those in the sham operation group, and CAlb and QAlb in the Shenxiong group were lower than those in the hypoperfusion group, with statistical significance (P ＜ 0.05). There was no significant difference in SAlb between the hypoperfusion group and sham operation group (P ＞ 0.05). There was no significant difference in SAlb between the Shenxiong group and hypoperfusion group (P ＞ 0.05). The mRNA and fluorescence intensity of ZO-1 and Occludin in the hypoperfusion group were lower than those in the sham operation group, and the mRNA and fluorescence intensity of ZO-1 and Occludin in the Shenxiong group were higher than those in the hypoperfusion group, with statistical significance (P ＜ 0.05). Conclusion The permeability of blood-cerebrospinal fluid barrier increased in rats under hypoperfusion state, and Shenxiong Glucose Injection had protective effect on blood-cerebrospinal fluid barrier to some extent.
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