|
|
|
| Study on the mechanism of Chaihu Guizhi Decoction in the treatment of depression based on network pharmacology and molecular docking technology#br# |
| LAN Shaohang1 LUO Jun1,2 LI Nana1 LUO Yehao1 CHEN Qiuxia1 CHEN Jingqin1 LI Hongyi1 FANG Gang1,3 |
1.Key Laboratory of Applied Basic Research of Guangxi Zhuang Medicine, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530001, China;
2.Modern Educational Technology and Information Center, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530200, China;
3.Guoyitang, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530011, China |
|
|
|
Abstract Objective To study the mechanism of Chaihu Guizhi Decoction in the treatment of depression by network pharmacology and molecular docking technology. Methods Traditional Chinese medicine systems pharmacology database and analysis platform was used to collect the main chemical components and targets of nine traditional Chinese medicines in Chaihu Guizhi Decoction. Depression related targets were obtained from GeneCards, pharmacogenomics knowledgebase, online Mendelian inheritance in man, DrugBank, TTD, and other databases. Proteinprotein interaction network of potential targets was constructed by using STRING online platform, and GO functional enrichment analysis and KEGG pathway analysis were obtained by R language. The “active component-disease target” network was constructed and topologically analyzed by Cytoscape3.7.2. Finally, AutoDock vina and other software were used to verify the molecular docking between the active ingredients with high network intermediate value and the core target. Results A total of 154 chemical constituents including quercetin and kaempferol were screened and 250 related targets were identified, among which the key targets were JUN, Akt1, and ESR1. A total of 2900 enrichment results were obtained by GO functional enrichment analysis, which mainly involved the regulation of biological processes such as lipopolysaccharide response and oxidative stress response. A total of 170 pathways were enriched by KEGG pathway analysis, mainly involving MAPK signaling pathway, PI3K-Akt signaling pathway, etc. The molecular docking results showed that the main active ingredients had strong binding ability with the core target. Conclusion This study preliminarily shows that Chaihu Guizhi Decoction plays a synergistic role in the treatment of depression through multiple components, multiple targets and multiple pathways.
|
|
|
|
|
|
[1] 王静，孔令茵，雷炳业，等.抑郁症的脑复杂网络研究进展[J].中国医学物理学杂志，2020，37（6）：780-785.
[2] 赵谦，王安娜，高雪松，等.慢性不可预知性温和应激致抑郁样大鼠模型的研究进展[J].实验动物与比较医学，2020，40（4）：344-353.
[3] 高明周，高冬梅，杨焕新，等.中医药柴胡类方抗抑郁现状研究与思考[J].中华中医药杂志，2018，33（9）：3991-3994．
[4] 赵艳青，滕晶，杨洪军.基于数据挖掘的现代中医药治疗抑郁症用药规律分析[J].中国中药杂志，2015，40（10）：2042-2046.
[5] 赵智勇，耿慧春，姚兵，等.柴胡桂枝汤抗抑郁作用的实验研究[J].中医药信息，2006（3）：50-51.
[6] 李耀洋，尚立芝，许二平.仲景方药辨治抑郁症研究进展[J].中国实验方剂学杂志，2020，26（23）：1-11.
[7] 肖頔，葛鑫宇，黎绢花.柴胡桂枝汤联合文拉法辛治疗中重度抑郁症的临床研究[J].中西医结合心脑血管病杂志，2019，17（20）：3215-3217.
[8] 周培培，郝巨辉.郝万山教授论治抑郁性失眠[J].现代中西医结合杂志，2018，27（16）：1748-1750.
[9] 史国军，施航，叶兴涛，等.柴胡桂枝汤加味治疗乳腺癌伴抑郁症36例[J].浙江中医杂志，2017，52（1）：36-37.
[10] 葛鑫宇，于林，蔡俊逸，等.柴胡桂枝汤治疗抑郁症40例临床观察[J].中国临床研究，2014，27（5）：614-615.
[11] Cheng BF，Hou YY，Jiang M，et al. Anti-inflammatory mechanism of Qingfei Xiaoyan Wan studied with network pharmacology [J]. Acta Pharm Sin，2013，48（5）：686-693．
[12] Gao W，Wang W，Peng Y，et al. Antidepressive effects of kaempferol mediated by reduction of oxidative stress，proinflammatory cytokines and up-regulation of AKT/β-catenin cascade [J]. Metab Brain Dis，2019，34（2）：485-494.
[13] 杜晨晖，崔小芳，裴香萍，等.酸枣仁皂苷类成分及其对神经系统作用研究进展[J].中草药，2019，50（5）：1258-1268.
[14] Zeng B，Li Y，Niu B，et al. Involvement of PI3K/Akt/FoxO3a and PKA/CREB signaling pathways in the protective effect of fluoxetine against corticosterone-induced cytotoxicity in PC12 cells [J]. J Mol Neurosci，2016，59（4）：567-578.
[15] 张文丽，唐慧，张瑞，等．中枢神经系统中雌激素的研究进展[J].中国老年学杂志，2016，36（20）：5184-5186．
[16] 张磊阳，蒋健，贺敏，等.抗抑郁中药的药理研究进展[J].中国实验方剂学杂志，2017，23（24）：224-234.
[17] 王珑，王实涛.抑郁症氧化应激发病机制及针刺治疗研究进展[J].针灸临床杂志，2017，33（11）：76-80.
[18] Brunoni AR，Baeken C，Machado-Vieira R，et al. BDNF blood levels after electroconvulsive therapy in patients with mood disorders：a systematic review and metaanalysis [J]. World J Biol Psychiatry，2014，15（5）：411-418．
[19] Lu J，Yang XY，Hua Q，et al. cAMP R esponse element binding protein：antidepressants intersection signal transduction pathways [J]. Prog Phys Sci，2008，39（4）：371-374．
[20] 胡莺燕，洪武，方贻儒.脑源性神经营养因子及其信号通路与抑郁症[J].上海精神医学，2009，21（3）：185-187.
[21] 王梅，程韬，杨开仁，等.抑郁症患者血清脑源性神经生长因子、P物质、白细胞介素-6水平变化及临床意义[J].中国医药导报，2019，16（13）：95-98.
[22] 黄芸，张治楠，黄泳，等.PI3K-AKT信号通路与抑郁症的关系及中医干预作用研究进展[J].上海中医药杂志，2020，54（2）：108-112.
[23] 孟盼，杜青，张秀丽，等.百事乐胶囊对慢性应激抑郁大鼠海马磷脂酰肌醇-3激酶信号通路相关因子的影响[J].中国中医药信息杂志，2017，24（8）：41-45.
[24] 吴丹，高耀，向欢，等.PI3K/Akt信号通路在抑郁症及抗抑郁中药作用机制研究中的进展[J].中草药，2019，50（18）：4461-4469.
[25] 吴丹，高耀，向欢，等.基于网络药理学的柴胡抗抑郁作用机制研究[J].药学学报，2018，53（8）：210-219. |
|
|
|
