新西兰家兔颈动脉体外应力培养研究

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Abstract Objective To study the changes of vascular structure in vitro stress culture of carotid artery in New Zealand rabbits by adjusting the parameters of fluid flow, flow rate and periodic pulsation. Methods Carotid arteries of SPF New Zealand rabbits were put in a bioreactor to establish the vessel culture model in vitro. There were four groups, the static group was static culture, and the dynamic groups (group 80, group 50 and group 25) were maintained under the same operating displacement and frequency of pulse pump, and the flow rates of culture medium were 80, 50 and 25 ml/min, respectively, to maintain the stress state culture. After the culture, the changes of vascular structure were evaluated by HE, Masson and EVG staining. Results In static group, vascular endothelial cells remained intact, smooth muscle cells were arranged in disorder, elastic fibers contracted sharply, and collagen fibers remained basically unchanged at 15 days. In group 80, elastic fibers did not change significantly, and endothelial cells could not be seen，and the smooth muscle in the 8th day was more orderly than that in the 4th day, and collagen fibers decreased significantly in the 8th day. In group 50, endothelial cells were not visible, smooth muscle cells were arranged in order, elastic fibers became loose and flat at the 15th days, and vascular collagen fibers decreased significantly. In group 25, the changes of elastic fibers and collagen fibers were milder than those in group 50. Conclusion Under the condition of simulating various parameters in vivo, the dynamic culture of blood vessels in vitro is helpful to maintain the physiological structure of blood vessels. The difficulty of culture is to prevent pollution and supplement collagen. Under the condition of high stress, vascular endothelial cells will be damaged, resulting in the proliferation of smooth muscle cells and the more serious loss of collagen fibers.

Key words： Blood vessel culture in vitro Stress Bioreactor Elastic fiber Collagen fiber Vascular endothelial cell

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	FU Haiyang1 LI Min1
	2 YU Hao1
	2 FU Bufang1 WANG Zhaoxu1

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FU Haiyang1 LI Min1,2 YU Hao1,2 FU Bufang1 WANG Zhaoxu1. Study of the in vitro stress culture of New Zealand rabbit carotid artery[J]. 中国医药导报, 2021, 18(31): 4-6,16.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I31/4

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