参苓白术丸对原发性高胆固醇血症豚鼠模型的影响

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Abstract
Objective To investigate the effect of Shenling Baizhu Pills on guinea pigs model with primary hypercholesterolemia. Methods Sixty male Hartley guinea pigs were divided into control group, model group, Ezetimibe group, Shenling Baizhu Pills high-dose, medium-dose, and low-dose groups, with ten guinea pigs in each group. The control group was given ordinary diet, and the other groups were given high fat diet to establish the guinea pigs model of exogenous hypercholesterolemia. Four weeks after modeling, drug gavage intervention was started. Ezetimibe group was given Ezetimibe suspension 1 mg/kg, Shenling Baizhu pills high-dose, medium-dose, and low-dose groups were given 17.00, 8.50 and 0.85 g/kg, respectively, model group and control group were given equal volume of normal saline. The levels of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), bile TC and total bile acid were compared. The histomorphological changes of liver in each groups were observed. Meanwhile, the expression levels of Niemann-Pick type C1 like protein 1 (NPC1L1), ATP binding cassette transport G5 (ABCG5), acyl-CoA cholesterol acyltransferase 2 (ACAT2) and liver X receptor α (LXRα) in each groups were recorded. Results Compared with control group, the serum levels of TG, TC, LDL-C, bile TC, and total bile acid in model group were increased (P < 0.05). Compared with model group, the level of serum TG in Shenling Baizhu Pills low-dose group was decreased (P < 0.05); the levels of TG and LDL-C in Shenling Baizhu Pills medium-dose group were decreased, while the level of HDL-C was increased (P < 0.05); the serum levels of TG, TC, and LDL-C in Shenling Baizhu Pills high-dose group were decreased, while the level of HDL-C was increased (P < 0.05); the levels of bile TC and total bile acid in Shenling Baizhu Pills medium-dose and high-dose groups were decreased (P < 0.05). The liver tissue of control group was normal; in model group, diffuse bullae steatosis, extensive balloonlike change of liver cells, a small amount of inflammatory cell infiltration and dot cell necrosis were observed; steatosis and hepatocyte balloon-like change were significantly reduced in Shenling Baizhu Pills medium-dose and high-dose groups; the improvement of hepatic adipose degeneration in Shenling Baizhu Pills low-dose group was not obvious compared with model group. Compared with control group, the levels of NPC1L1, ACAT2, ABCG5, and LXRα in model group were increased (P < 0.05). Compared with model group, the levels of NPC1L1, ACAT2, and LXRα in Shenling Baizhu Pills medium-dose and high-dose groups were decreased, while the levels of ABCG5 were increased (P < 0.05). Conclusion Shenling Baizhu Pills medium-dose and high-dose can alleviate hyperlipidemia induced by high cholesterol diet and reduce hepatocyte steatosis, inhibit the expression of NPC1L1, ACAT2, and LXRα in small intestine of guinea pigs model with primary hypercholesterolemia, and up-regulate the expression of ABCG5.

Key words： Shenling Baizhu Pills Primary hypercholesterolemia Niemann-Peak C1 like protein 1 Acyl-CoA cholesterol acyltransferase 2 ATP binding cassette transport G5

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	ZHANG Yutian1 WANG Hong2 QU Ziyi1 YANG Chaoshuai2 FENG Binbin1 WANG Yuehao1

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ZHANG Yutian1 WANG Hong2 QU Ziyi1 YANG Chaoshuai2 FENG Binbin1 WANG Yuehao1. Effect of Shenling Baizhu Pills on guinea pigs model of primary hypercholesterolemia#br#[J]. 中国医药导报, 2021, 18(30): 33-37.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I30/33

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