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| Effect of astragalus polysaccharide on gastrointestinal function regulation and repair mechanism of intestinal mucosa injury in rats with dampness stagnancy due to spleen deficiency#br# |
| YANG Binbin1 CUI Ning1 ZHANG Yanan2 ZHAO Wenxiao3 WANG Shijun2 |
1.College of Health Sciences, Shandong University of Traditional Chinese Medicine, Shandong Province, Jinan 250355, China;
2.College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
3.School of Nursing, Shandong University of Traditional Chinese Medicine, Shandong Province, Jinan 250355, China |
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Abstract Objective To investigate the regulating effect of astragalus polysaccharide (APS) on gastrointestinal function in rats with dampness stagnancy due to spleen deficiency and the mechanism of repairing intestinal mucosal injury through Wnt/β-catenin signaling pathway. Methods Fifty 4-week-old male Wistar rats were divided into five groups according to random number table method, with ten rats in each group: control group (normal feeding), model group (rat model of dampness stagnancy due to spleen deficiency induced by high-fat and low-protein diet supplemented by exhaustion swimming), APS high-dose group (after modeling, APS was given 900 mg/[kg·d], 1 ml/100 g each time), APS medium-dose group (after modeling, APS was given 600 mg/[kg·d], 1 ml/100 g each time), and APS low-dose group (after modeling, APS was given 300 mg/[kg·d], 1 ml/100 g each time), APS was administered daily. Control group and model group were given the same volume of normal saline intragastric administration for two weeks. Serum amylase, D-xylose, motilin and gastrin were detected by enzyme-linked immunoadsordent assay. HE staining was used to observe the pathological changes of small intestine in each group. The changes of Wnt1 and β-catenin protein in small intestine tissue were observed by immunohistochemistry. Results Compared with the control group, the levels of serum amylase, D-xylose, motilin and gastrin in model group were significantly decreased, with highly statistical significance (P ＜ 0.01). Compared with model group, serum amylase, D-xylose and gastrin levels in APS high-dose group were increased, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The levels of serum amylase and motilin in APS middle-dose and APS low-dose groups were increased, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The results of HE staining showed that the intestinal mucosa of the model group was damaged, and the APS dose groups were improved in different degrees. Compared with control group, the expression of Wnt1 and β-catenin protein in small intestine of model group was significantly up-regulated, and the difference was highly statistically significant (P ＜ 0.01). Compared with model group, the protein expressions of Wnt1 and β-catenin in APS dose groups were significantly down-regulated, with highly statistical significance (P ＜ 0.01). Conclusion APS can improve gastrointestinal digestion and absorption function in rats with dampness and spleen deficiency, and its mechanism is related to the regulation of Wnt /β-catenin pathway.
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