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| Effects of Chaihuan Ningshen Granules on the behavior and hypothalamic-pituitary-adrenal axis of rats with insomnia with syndrome of stagnation of liver qi |
| DU Jianeng1 NIE Chunxia1 XU Wenwen2 LIU Cong2 KANG Yong2 NI Yan2 HAO Xuliang2 |
1.Graduate Faculty, Shanxi University of Chinese Medicine, Shanxi Province, Taiyuan 030619, China;
2.Institute of Chinese Prescription, Shanxi Institute of Traditional Chinese Medicine, Shanxi Province, Taiyuan 030045, China |
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Abstract Objective To investigate the effects of Chaihuan Ningshen Granules on the behavior and hypothalamic-pituitary-adrenal (HPA) axis of rats with insomnia with syndrome of stagnation of liver qi induced by multifactor combination therapy. Methods Seventy SD rats (half male and half female) were randomly divided into 7 groups: blank group, model group, high, medium and low dose of Chaihuan Ningshen Granules groups (2.25, 1.125, 0.5625 g/kg), Jieyu Anshen Granules group (1.25 g/kg), Estazolam group (0.5 mg/kg). Except for the blank group, rats in each group were established for the model with syndrome of stagnation of liver qi by multifactorial stimulation including tie, pinch-tail and PCPA. Each administration group was treated daily with an oral infusion of corresponding drug, whereas, the blank group and the model group were given equal volume of saline once a day for 14 days. All rats were tested on the 0, 7th, 14th, 21st d for body weight, sugar consumption, sleep status, water maze navigation and spatial search ability to evaluate the behavior of rats, The levels of corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and serum cortisol (CORT) in the hypothalamus of rats were detected by enzyme linked immunosorbent assay (ELISA). Results Compared with blank group, the body weight, sugar consumption, sleep duration and spatial search ability of the model group were significantly decreased, while the hypothalamic CRH, plasma ACTH, serum CORT and water maze positioning sailing time were markedly increased (P ＜ 0.05). Compared with the model group, all medicine groups could significantly increase body weight, sleep duration, consumption of sugar water and the number of crossing platform (P ＜ 0.05), meanwhile decrease the levels of the hypothalamic CRH, plasma ACTH and serum CORT, as well as water maze positioning sailing time, the differences were statistically significant (P ＜ 0.05). Conclusion Chaihuan Ningshen Granules can remarkably improve the behavior of rats with insomnia with syndrome of stagnation of liver qi, and prolong their sleep time, the mechanism underlying this event is mainly dependent on the improvement effect of Chaihuan Ningshen Granules on the function of HPA axis.
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