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Effects of Yunzhi Wuweizi Extract on liver injury induced by Sulfasalazine in mice |
YU Haitao1 WANG Qunying1 MIAO Qing2 DONG Wenzhu1 |
1.Department of Gastroenterology, No.971 Hospital of Chinese People’s Liberation Army Navy, Shandong Province, Qingdao 266071, China;
2. Department of Pharmacy, No.971 Hospital of Chinese People’s Liberation Army Navy, Shandong Province, Qingdao 266071, China |
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Abstract Objective To study the effects of Yunzhi Wuweizi Extract on liver injury induced by Sulfasalazine in mice. Methods Sixty 6-week-old male ICR mice weighing 18-22 g were randomly divided into six groups by numbering method. Control group, model group, Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dosage groups, with 10 mice in each group. Model group, Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dose groups were intragastric with Sulfasalazine solution (1000 mg/kg, suspended in 0.5% sodium carboxymethyl cellulose [CMC-Na]) every day to prepare drug liver injury model. Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and highdose groups were given ursodeoxycholic acid (10 mg/kg) and Yunzhi Wuweizi Extract solution (50, 100, 300 mg/kg) by intragastric administration 2 h after Sulfadiazine administration, respectively. Model group and control group were given isopyknic 0.5% CMC-Na solution. The intervention lasted for 14 days. On the 15th day, after blood was collected from eyeballs, the serum of each group was separated and determined for glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), and alkaline phosphatase (ALP). The liver tissues of each group were stained with hematoxylin-eosin (HE) to observe the pathological changes of the liver. Results The levels of GPT, GOT, and ALP in model group were higher than those in control group, and the differences were statistically significant (P < 0.05). The levels of GPT, GOT, and ALP of Yunzhi Wuweizi Extract in low, medium, and high dose groups were lower than those in model group, and those in Yunzhi Wuweizi Extract medium and high dose groups were lower than those in Yunzhi Wuweizi Extract low dose group, with statistical significance (P < 0.05 or P < 0.01). HE staining results showed that hepatic cell swelling, cell membrane detachment and rupture in model group, and liver injury was significantly improved in Ursodeoxycholic acid group and Yunzhi Wuweizi Extract low, medium, and high dose groups. Conclusion Yunzhi Wuweizi Extract has protective effect on liver injury induced by sulfasalazine in mice.
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[1] Leise MD,Poterucha JJ,Talwalkar JA. Drug-induced liver injury [J]. Mayo Clin Proc,2014,89(1):95-106.
[2] Low EXS,Zheng Q,Chan E,et al. Drug induced liver injury:East versus West-a systematic review and meta-analysis [J]. Clin Mol Hepatol,2020,26(2):142-154.
[3] 吴聪,杨芳,丁长玲,等.柳氮磺吡啶致肝功能衰竭一例并文献复习[J].中国实用医刊,2019(2):123-126.
[4] 刘田,王秀茹,安媛,等.柳氮磺吡啶在我国类风湿关节炎患者的用药现况调查[J].北京大学学报医学版,2012, 44(2):188-194.
[5] Heidari R,Rasti M,Shirazi Yeganeh B,et al. Sulfasalazine-induced renal and hepatic injury in rats and the protective role of taurine [J]. Bioimpacts,2016,6(1):3-8.
[6] 莫永炎,陈瑗,周玫,等.云芝多糖对脑、肝组织的抗氧化作用研究[J].中国药理学通报,2001,17(6):628-631.
[7] 徐惠波,牛晓晖,纪凤兰,等.云芝多糖对小鼠NK细胞与单核吞噬细胞系统的影响[J].中药药理与临床,2006, 22(5):25-26.
[8] 李玲艳,包海鹰.云芝的化学成分及药理活性研究概况[J].菌物研究,2011,9(3):180-186.
[9] 王康乐,陆震鸣,陈露.云芝多糖组分对酒精性肝损伤小鼠的保肝活性测试[J].食药用菌,2018,26(4):235-239.
[10] 赵新湖,包海鹰.云芝对对乙酰氨基酚肝损伤的保护作用[J].时珍国医国药,2015,26(7):1633-1634.
[11] 冯耀培,宋娟,郑斯文,等.云芝浸膏对小鼠CCl4肝损伤保护作用机制研究[J].特产研究,2020,42(1):15-20.
[12] 冯耀培,郑斯文,郑培和,等.云芝五味子浸膏对小鼠急性酒精肝损伤的保护作用[J/OL].吉林农业大学学报:1-9[2021-08-30].https://doi.org/10.13327/j.jjlau.2019.4857.
[13] 国家药典委员会.中华人民共和国药典[M].一部.北京:中国医药科技出版社,2015:60.
[14] de Abajo FJ,Montero D,Madurga M,et al. Acute and clinically relevant drug-induced liver injury:a population based case-control study [J]. Clin Pharmacol,2004, 58(1):71-80.
[15] Khokhar OS,Lewis JH. Hepatotoxicity of agents used in the management of inflammatory bowel disease [J]. Dig Dis,2010,28(3):508-518.
[16] Marinos G,Riley J,Painter DM,et al. Sulfasalazine-induced fulminant hepatic failure [J]. Clin Gastroenterol,1992,14(2):132-135.
[17] Rubin R. Sulfasalazine-induced fulminant hepatic failure and necrotizing pancreatitis [J]. Am J Gastroenterol,1994, 89(5):789-791.
[18] 周仲强,魏恩巍.动物肝损伤模型建立的研究进展[J].吉林化工学院学报,2018,35(11):14-17.
[19] Hilzenrat N,Lamoureux E,Sherker A,et al. Cholestasis in Crohn’s disease:a diagnostic challenge [J]. Can J Gastroenterol,1997,11(1):35-37.
[20] 尤梅桂.熊去氧胆酸的研究概况[J].药学研究,2021,40(3):199-202.
[21] 白玉莹,葛唯佳,蔡恩博,等.杂色云芝粗多糖预防性给药对扑热息痛诱导的肝损伤小鼠的肝保护作用[J].吉林农业大学学报,2020,42(1):56-61.
[22] 王陈萍,宣东平,陈霞,等.五味子醇甲和五味子甲素对四氯化碳所致小鼠急性肝损伤的保护作用及机制[J].中国临床药理学杂志,2019,35(8):791-794.
[23] 陈宝芝,李生,吴金滢,等.北五味子粗多糖对四氯化碳致小鼠急性肝损伤的保护作用及其机制[J].吉林大学学报医学版,2014,40(1):92-96.
[24] 燕菲,张巧艳,张宏,等.五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J].药学实践杂志,2009, 27(5):340-344.
[25] 付冉,李日,王小楠,等.五味子对肝损伤的保护作用及其分子机制研究进展[J].中国医院用药评价与分析,2021, 21(6):761-763. |
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