槲皮素的抗炎免疫及部分机制研究

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Abstract Objective To investigate the anti-inflammatory and immune effects of quercetin and its partial mechanism. Methods Different concentrations of quercetin were used to intervene RAW 264.7 cells to observe the effects of quercetin on the viability of RAW 264.7 cells. Quercetin with no influence on the viability of RAW 264.7 cells was selected for further study. Untreated RAW 264.7 cells were used as blank group, and the inflammatory cell model was established by stimulating RAW 264.7 cells with 1 μg/ml lipopolysaccharide (LPS). The cells were divided into model group, quercetin 0.98, 1.95, 3.90, and 7.80 μg/ml dose groups. Cell viability, reactive oxygen species (ROS), and monocyte chemoattractant protein 1 (MCP1) levels were observed. The cyclooxygenase (COX)-1 and COX-2 inhibitor screening kit was used to detect the inhibition of quercetin on COX-1 and COX-2. In addition, 20 SPF BALB/C male mice (6-8 weeks old, 18-22 g body weight) were selected to prepare splenic lymphocyte suspension, and different concentrations of quercetin were used to interfere with the splenic lymphocytes of mice, and the effects of quercetin on cell proliferation were observed. Mice spleen lymphocytes were respectively treated with LPS (10 μg/ml) or concanavalin A (Con A) (8 μg/ml) were divided into LPS group, Con A group, and quercetin 0.98, 1.95, 3.90, 7.80 μg/ml groups. The effects of different concentrations of quercetin on the proliferation of spleen lymphocytes, T cells induced by Con A and B cells induced by LPS, and the levels of tumor necrosis factor -α (TNF-α), interleukin-6 (IL-6) and interferon γ (INF-γ) in T cells were observed. Results When quercetin ＜ 7.8 μg/ml, there was no significant difference in the activity of RAW 264.7 cells compared with untreated cells (P ＞ 0.05). The levels of ROS and MCP1 in quercetin 0.98, 1.95, 3.90, and 7.80 μg/ml groups were lower than those in model group, and the difference was highly statistically significant (P ＜ 0.01). The IC50 of quercetin to COX-1, COX-2 were 12.73 and 0.44 μg/ml, respectively, and the IC50 ratio of COX-2 to COX-1 was 0.034. The proliferation of spleen lymphocytes of mice with quercetin concentration of 0.98, 1.95, 3.90, and 7.80 μg/ml were higher than those without quercetin, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). T cells in quercetin 1.95, 3.90, and 7.80 μg/ml dose groups were lower than those in Con A group, and B cells in quercetin 3.90 and 7.80 μg/ml dose groups were lower than those in LPS group, with statistical significance (P ＜ 0.05). The levels of TNF-α in quercetin 1.95, 3.90, and 7.80 μg/ml groups were lower than those in Con A group, the leves of IL-6 and INF-γ in quercetin dose groups were lower than those in Con A group, and the differences were highly statistically significant (P ＜ 0.01). Conclusion Quercetin is a selective COX-2 inhibitor with good anti-inflammatory activity, which can promote the proliferation of splenic lymphocytes in mice, inhibit the proliferation of T and B cells and the secretion of cytokines.

Key words： Quercetin RAW 264.7 cell Anti-inflammatory Immune

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	SI Lijun WANG Xue WANG Linlin HUANG Hua

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SI Lijun WANG Xue WANG Linlin HUANG Hua. Research on anti-inflammatory and immune effects of quercetin and its partial mechanism[J]. 中国医药导报, 2021, 18(27): 26-29,34.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I27/26

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