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| Effects and mechanism of Xuesaitong Injection on expressions of VEGF pathway in mice brain microvascular endothelial cells after OGD/R |
| GUO Yangyan SUN Xingyun MENG Fanxing WANG Le LI Nannan |
| The First Department of Neurology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China |
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Abstract Objective To study the effect of Xuesaitong Injection on the expression of vascular endothelial growth factor (VEGF), VEGFR-2 and soluble Fms-like tyrosine kinase 1 (sFlt-1) protein in brain microvascular endothelial cells of mice injured by oxygen glucose deprivation/reoxygenation (OGD/R), and to explore its influence on the expression of VEGF pathway and its possible mechanism. Methods The OGD/R method was used to establish a cell ischemia-reperfusion injury model, and the cell viability was detected by the CCK-8 method to determine the optimal cell seeding concentration, reoxygenation time and Xuesaitong Injection concentration. The experiment was divided into normal group, model building group, and experimental group. The normal group was cultured normally, and the model building group was only cultured without drugs, while the experimental group was cultured with Xuesaitong Injection after the model was built. Western blot method was used to detect the expression of VEGF, VEGFR-2, and sFlt-1 protein in the three groups of cells. Results After six hours of oxygen and glucose deprivation, the optimal cell seeding plate concentration was 2.5×104 cells/ml, the reoxygenation time was six hours, and the concentration of Xuesaitong Injection was 100 mg/L. Under this condition, compared with the normal group, the expression of VEGF, VEGFR-2, and sFlt-1 protein in the model and the experimental group were significantly increased, and the differences were statistically significant (P ＜ 0.05); compared with the model, the expression of VEGF and VEGFR-2 protein in the experimental group were significantly increased, and the differences were statistically significant (P ＜ 0.05), and the expression of sFlt-1 protein was significantly reduced, and the difference was statistically significant (P ＜ 0.05). Conclusion Xuesaitong Injection can up-regulate the expression of VEGF pathway signals in OGD/R-injured mouse brain microvascular endothelial cells. The mechanism of up-regulating VEGF pathway signal expression may be related to the inhibition of sFlt-1 protein expression.
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[1] 张宏欣.脑梗死的流行病学及危险因素调查[J].继续医学教育，2020，34（2）：66-68.
[2] 国家卫生健康委员会.中国卫生健康统计年鉴2019[M].北京：中国协和医科大学出版社，2019：304.
[3] 彭琴，李海玲，王媛，等.1990-2016年中国人群脑血管病疾病负担变化趋势[J].中华流行病学杂志，2019，40（4）：400-405.
[4] 高长玉，吴成翰，赵建国，等.中国脑梗死中西医结合诊治指南（2017）[J].中国中西医结合杂志，2018，38（2）：136-144.
[5] 黄宁芳，颜钟生，唐国传，等.血塞通对急性脑梗死不同时间点血清VEGF和VEGFR表达的影响[J].中国社区医师，2015，31（11）：104-106.
[6] Cim N，Kurdoglu M，Ege S，et al. An analysis on the roles of angiogenesis-related factors including serum vitamin D，soluble endoglin（sEng），soluble fms-like tyrosine kinase 1（sFlt1），and vascular endothelial growth factor（VEGF）in the diagnosis and severity of late-onset preeclampsia [J]. J Matern Fetal Neonatal Med，2017，30（13）：1602-1607.
[7] 张伯礼，吴勉华.中医内科学[M].北京：中国中医出版社，2017：127-134.
[8] 彭斌，吴波.中国急性缺血性脑卒中诊治指南2018[J].中华神经科杂志，2018，51（9）：666-682.
[9] 梁庆.脑梗死缺血/再灌注损伤机制的研究进展[J].医学理论与实践，2014，27（24）：3263-3266.
[10] Mayer-Pickel K，Kolovetsiou-Kreiner V，Stern C，et al. Effect of Low-Dose Aspirin on Soluble FMS-Like Tyrosine Kinase 1/Placental Growth Factor （sFlt-1/PlGF Ratio） in Pregnancies at High Risk for the Development of Preeclampsia [J]. J Clin Med，2019，8（9）：1429.
[11] Wang X，Freire Valls A，Schermann G，et al. YAP/TAZO-rchestrate VEGF Signaling during Developmental Angiogenesis [J]. Dev Cell，2017，42（5）：462-478.
[12] Lin YC，Chao TY，Yeh CT，et al. Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis [J]. Arterioscler Thromb Vasc Biol，2017， 37（1）：144-155.
[13] Tae N，Lee S，Kim O，et al. Syntenin promotes VEGF-induced VEGFR2 endocytosis and angiogenesis by increasing ephrin-B2function in endothelial cells [J]. Oncotarget，2017，8（24）：38886-38901.
[14] Xie C，Wan X，Quan H，et al. Preclinical characterization of anlotinib，a highly potent and selective vascular endothelial growth factor receptor-2 inhibitor [J]. Cancer Sci，2018，109（4）：1207-1219.
[15] 吴华英，向德兵.血管内皮生长因子在肿瘤血管生成的研究进展[J].中国肿瘤临床与康复，2012，19（5）：470-471.
[16] Andraweera PH，Dekker GA，Roberts CT. The vascular endo-thelial growth factor family in adverse pregnancy outcomes [J]. Hum Reprod Update，2012，18（4）：436-457.
[17] Dumnicka P，Kusnierz-Cabala B，Sporek M，et al. Serum Concentrations of Angiopoietin-2 and Soluble fms-Like Tyrosine Kinase 1 （sFlt-1） Are Associated with Coagulopathy among Patients with Acute Pancreatitis [J]. Int J Mol Sci，2017，18（4）：753.
[18] 范崇桂，付国惠，闪海霞，等.血塞通对局灶性脑缺血再灌注大鼠VEGF/VEGFR2的调节[J].热带医学杂志，2020， 20（1）：13-17，22，145.
[19] 国家药典委员会.中华人民共和国药典[M].一部.北京：中国医药科技出版社，2020.
[20] 梁萍，蒙兰青.三七治疗缺血性脑卒中药理机制的研究进展[J].山东医药，2020，60（16）：95-98.
[21] 任珊.结合功能磁共振评价联合三七总皂苷对中风病患者神经重塑的研究[D].北京：北京中医药大学，2017.
[22] 林军.三七总皂苷对急性脑缺血后神经血管单元的影响及作用机制研究[D].百色：右江民族医学院，2018.
[23] Zhang JS，Zhang BX，Du MM，et al. Chinese prepa- ration Xuesaitong promotes the mobilization of bone marrow mesen-chymal stem cells in rats with cerebral infarction [J]. Neural Regen Res，2016，11（2）：292-297.
[24] 刘南暖，陈艳，庄凤娟，等.血塞通注射液联合奥扎格雷钠治疗急性脑梗死有效性与安全性的Meta分析[J].中国中医急症，2019，28（3）：382-386.
[25] 李苗，胡如，张召平.血塞通联合依达拉奉治疗脑梗死患者的临床效果[J].医疗装备，2020，33（18）：104-105.
[26] 孙行云，刘霞，孟繁兴，等.联合血塞通对比单纯西药治疗脑梗塞临床疗效的Meta分析[J].昆明医科大学学报，2020，41（8）：18-26.
[27] Zhang W，Smith C，Shapiro A，et al. Increased expression of bioactive chemokines in human cerebromicrovascular endothelial cells and astrocytes subjected to simulated ischemia in vitro [J]. J Neuroimmunol，1999，101（2）：148-160. |
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