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| Observation on the formation and division of multinucleated giant cells in cell damage microenvironment |
| WANG Jia YE Dongmei YUAN Xiaolin |
| Central Laboratory, Affiliated Zhongshan Hospital of Dalian University, Liaoning Province, Dalian 116001, China |
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Abstract Objective To observe the formation process of multinucleated giant cells (MGC) in cell damage microenvironment, and to provide scientific basis for the study of the mechanism of MGC formation. Methods Macrophages (RAW 264.7) and cardiomyocytes (HL-1) were co-cultured in the ratio of 1∶3 for 15 days. The experimental group did not change the fluid without passage, and the control group changed the fluid regularly. MGC formation in cell injury microenvironment was observed. The MGC formation process and the interaction between the two kinds of cells were observed by a live cell workstation. The cell fusion was confirmed by scanning electron microscopy. Results In the cell damage microenvironment, MGC was formed and the number of cells increased with the prolongation of the time of cessation of fluid change. The dynamic process of MGC formation was observed under light microscope. The whole process of MGC formation and division and the formation of MGC by the fusion of staining cells were observed at the living cell workstation. The unstained cells were observed to form MGC by scanning electron microscope. Conclusion Macrophages fuse with damaged cardiomyocytes to form MGC and can induce its division and proliferation.
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