幽门螺杆菌毒力因子CagA对人正常胃上皮HFE145细胞CDX1表达及上皮间质转化的影响

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Abstract Objective To investigate the effect of Helicobacter pylori virulence factor cytotoxin related gene A (CagA) on cadual type homeobox transcription factor 1 (CDX1) expression and epithelial mesenchymal transition in human normal gastric epithelial HFE145 cells. Methods Human normal gastric epithelial HFE145 cells were divided into control group, negative group, CagA group and combined group. Lipofectamine 2000 was used to transfect blank reagent, pcDNA3.1, WT CagA, and WT CagA combined with SC-35731-V, respectively. The expression levels of CDX1, CagA, E-cadherin, Vimentin, and N-cadherin in transfected cells were detected by Western blot; invasion and migration ability of transfected cells were detected by Transwell chamber method; the potential binding sites of CagA related factors were determined by fluorescein. Results The expression level of CDX1 in CagA group was higher than that in control group and negative group (P < 0.05), while the expression level of CDX1 in combination group was lower than that in CagA group (P < 0.05). Invasion and migration ability of HFE145 cells in CagA group were higher than those in control group and negative group (P < 0.05), while invasion and migration ability of HFE145 cells in combination group were lower than that in CagA group (P < 0.05). The expression level of E-cadherin in CagA group was lower than that in control group and negative group (P < 0.05), while the expression level of E-cadherin in combination group was higher than that in CagA group (P < 0.05). The expression levels of Vimenti and N-cadherin in CagA group were higher than those in control group and negative group (P < 0.05), while the expression levels of Vimenti and N-cadherin in combination group were lower than those in CagA group (P < 0.05). Deletion of 501-1000 bp upstream of CDX1 exon did not inhibit luciferase activity, while deletion of 1-500 bp inhibited luciferase activity. Conclusion Helicobacter pylori virulence factor CagA induced the abnormal expression of CDX1, and the effect of targeted inhibition of CDX1 on the occurrence and development of gastric cancer has the value of further study.

Key words： Helicobacter pylori Cytotoxin related gene A Cadual type homeobox transcription factor 1 Gastric cancer

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	ZHANG Shanshan1 MIAO Feng1 LIU Lu1 LYU Wenyao2 ZHANG Shen1 ZHAO Zhifeng1

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ZHANG Shanshan1 MIAO Feng1 LIU Lu1 LYU Wenyao2 ZHANG Shen1 ZHAO Zhifeng1. Effect of CagA on CDX1 expression and epithelial mesenchymal transition in human normal gastric epithelial HFE145 cells[J]. 中国医药导报, 2021, 18(25): 35-39,43.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I25/35

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