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| Experimental study on antitumor effect and the effect of tumor immune microenvironment of Yanghe Decoction on Lewis lung cancer mice |
| WU Lin1 ZHANG Weibo2 XING Yanli1 LI Yan1 ZHAO Meifeng1 YAO Huan1 |
1.Department of Pharmacy, Xianyang Central Hospital, Shaanxi Province, Xianyang 712000, China;
2.Department of Pathology, Xianyang Central Hospital, Shaanxi Province, Xianyang 712000, China |
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Abstract Objective To investigate the antitumor effect and the effect of tumor immune microenvironment of Yanghe Decoction on Lewis lung cancer mice. Methods A total of 50 male C57BL/6 mice were subcutaneously inoculated with 0.2 ml Lewis lung cancer cell suspension at the concentration of 2×107/ml in the right anterior axilla to establish Lewis lung cancer mouse model. After the model was made, according to random number table method, these mice were divided into tumor-bearing model group, Cyclophosphamide group, Yanghe Decoction high concentration group, Yanghe Decoction medium concentration group， and low concentration group, with 10 mice in each group. Normal saline, 25 mg/kg Cyclophosphamide, 27.5, 18.3, and 9.2 g/kg Yanghe Decoction were given by intragastric administration for 10 days, respectively, one time per day. The body weight and tumor weight of mice in each group were observed. The expressions of interleukin (IL)-2, transforming growth factor (TGF)-β, and cytotoxic lymphocyte (CTL) and regulatory T cell (Treg) markers CD3+CD8+ CD4+Foxp3+ in tumor tissues were detected by immunohistochemistry and immunofluorescence, respectively. Results Compared with tumor-bearing model group, the body weight of mice in Yanghe Decoction high, medium concentration groups were increased (P < 0.05 or P < 0.01); compared with Cyclophosphamide group, the body weight of mice in Yanghe decoction high, medium, and low concentration groups were increased (P < 0.01); and compared with Yanghe Decoction high concentration group, the body weight of mice in Yanghe Decoction low concentration group was decreased (P < 0.05). Compared with tumor-bearing model group, tumor weight of mice in Yanghe Decoction high concentration group was decreased (P < 0.05); and compared with Cyclophosphamide group, tumor weight of mice in Yanghe Decoction medium, low concentration groups were increased (P < 0.05). The tumor inhibition rate from high to low was Cyclophosphamide group, Yanghe Decoction high concentration group, Yanghe Decoction medium concentration group and Yanghe Decoction low concentration group. Compared with tumor-bearing model group, the expression of IL-2 in Lewis lung cancer tissues of mice in Yanghe Decoction high, medium, and low concentration groups were increased (P < 0.05 or P < 0.01); compared with Cyclophosphamide group, the expression of IL-2 in Lewis lung cancer tissues of mice in Yanghe Decoction high, medium, and low concentration groups were increased (P < 0.01); and compared with Yanghe Decoction high concentration group, the expression of IL-2 in Lewis lung cancer tissues of mice in Yanghe Decoction medium, low concentration were decreased (P < 0.01). Compared with tumor-bearing model group, the expression of TGF-β in Lewis lung cancer tissue of mice in Yanghe Decoction high, medium concentration groups were decreased (P < 0.01); compared with Cyclophosphamide group, the expression of TGF-β in Lewis lung cancer tissues of mice in Yanghe Decoction medium, low concentration groups were increased (P < 0.05). Compared with tumor-bearing model group, the expression of CD3+CD8+ in Lewis lung cancer tissues of mice in Yanghe Decoction high, medium, and low concentration groups were increased (P < 0.05 or P < 0.01); compared with Cyclophosphamide group, the expression of CD3+CD8+ in Lewis lung cancer tissues of mice in Yanghe Decoction high, medium, and low concentration groups were increased (P < 0.01); and compared with Yanghe Decoction high concentration group, the expression of CD3+CD8+ in Lewis lung cancer tissues of mice in Yanghe Decoction medium, low concentration were decreased (P < 0.01). Compared with tumor-bearing model group, the expression of CD4+Foxp3+ in Lewis lung cancer tissues of mice in Yanghe Decoction high, medium concentration groups were decreased (P < 0.05 or P < 0.01). Conclusion Yanghe Decoction has a good anti-tumor effect on Lewis lung cancer mice without affecting their body weight, can increase the expression of IL-2 and CTL content in tumor immune microenvironment, and reduce the expression of TGF-β and the content of Treg in tumor immune microenvironment.
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