基于网络药理学探讨肾元颗粒治疗糖尿病肾病的作用机制

Abstract
Figure/Table
References (26)
Related Citation (15)

Download: PDF (1652 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the mechanism of action of Shenyuan Granules in treating diabetic nephropathy based on network pharmacology. Methods Through the traditional Chinese medicine systems pharmacology database and analysis platform, the pharmaceutical components and action targets of Shenyuan Granules were collected. Target genes of diabetic nephropathy were collected from GeneCards, OMIM, PharmGKB, TTD, and DrugBank databases, and the intersection of drug and disease targets was taken to obtain the intersection target of the prescription for treating diabetic nephropathy. Cytoscape 3.7.2 software was used to construct the Shenyuan Granules composition-intersection target-disease network. The STRING database was used to analyze the intersection targets and construct the protein interaction network. The CytoNCA topological properties were used to construct the topological map of key target proteins, and the intersection gene targets and compounds were ranked according to the nodal degree and intermediate centrality. GO biological processes and KEGG metabolic pathways of key targets were analyzed by R language. Results Thirty-five compounds were selected and 292 predicted targets of Shenyuan Granules were obtained. The number of intersection targets between drugs and diabetic nephropathy was 67. The intersection proteins were uploaded to the STRING database and 55 intersection proteins were obtained. The topology of key targets showed that the first three compounds were quercetin, 7-O-methylisopeugenol and kaempferol. Key targets were protein kinase B, vascular endothelial growth factor A and epidermal growth factor receptor. GO enrichment analysis showed that it mainly involved biological processes such as G-protein-coupled receptors, cellular components such as presynaptic membrane and postsynaptic membrane, and molecular functions such as G protein-coupled amine receptor activity. The enrichment analysis of KEGG metabolism pathway showed that it was mainly involved in stimulating neural tissue action pathway and so on. Conclusion The rationality of the prescription of Shenyuan Granules and the scientificity of the treatment of diabetic nephropathy are preliminarily verified, and the theoretical foundation is laid for the in-depth study of the mechanism of treating diabetic nephropathy with Shenyuan Granules.

Key words： Shenyuan Granules Diabetic nephropathy Network pharmacology Mechanism of action

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WAN Yaqin1 WANG Xiaoqin2

Cite this article:

WAN Yaqin1 WANG Xiaoqin2. Discussion on the mechanism of action of Shenyuan Granules in treating diabetic nephropathy based on network pharmacology[J]. 中国医药导报, 2021, 18(25): 14-18.

URL:

https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I25/14

[1] Fu H，Liu S，Bastacky SI，et al. Diabetic kidney diseases revisited：Anew perspective for a new era [J]. Mol Metab，2019，30：250-263.
[2] Luo TT，Lu Y，Yan SK，et al. Network Pharmacology in Research of Chinese Medicine Formula：Methodology，Application and Prospective [J]. Chin J Integr Med，2020，26（1）：72-80.
[3] 王岚，朱国双，孙龙，等.肾元颗粒对db/db糖尿病肾病小鼠血管钙化的改善作用及其机制[J].吉林大学学报：医学版，2020，46（3）：431-439.
[4] 朱国双，王岚，金善善，等.肾元颗粒通过调控FGF23/CaMKⅡ信号通路对糖尿病肾病db/db小鼠心肌肥大与凋亡的影响[J].中华中医药杂志，2020，35（5）：2401-2406.
[5] 朱国双，王岚，邹新蓉，等.基于FGF23-Klotho轴探讨肾元颗粒对db/db糖尿病肾病小鼠骨骼的保护作用[J].世界科学技术-中医药现代化，2020，22（1）：77-83.
[6] 陈立，王小琴，袁军，等.肾元颗粒含药血清对高糖诱导人肾小管上皮-间充质细胞转分化中Klotho/FGFR1/FGF2信号途径的影响[J].中国中西医结合杂志，2018，38（10）：1215-1221.
[7] 詹理睿，王小琴，邹新蓉，等.肾元颗粒影响糖尿病小鼠肾脏中miR-199b-5p与钙磷代谢的研究[J].中华中医药学刊，2017，35（11）：2819-2823。
[8] 陈立，王小琴.Klotho基因调控的FGF23/FGFR1信号通路对人肾小管上皮细胞羟化酶表达的影响及肾元颗粒的干预作用[J].中华中医药杂志，2019，34（10）：4547-4552.
[9] 肖晓芬，王小琴.基于klotho基因/FGF23信号通路研究中医药对糖尿病钙磷代谢的影响[J].临床肾脏病杂志，2015，15（6）：324-326.
[10] 陈立，袁军，詹理睿.FGF2/FGFR1/ERK信号通路在高糖诱导人肾小管上皮细胞转分化中的机制及肾元颗粒的干预作用[J].中国中西结合肾病杂志，2017，18（3）：201-204.
[11] 田密，雷琪，嫣韵升，等.山奈酚对脂肪酸诱导的胰岛微血管内皮功能损伤的保护效应及多聚腺苷二磷酸核糖聚合酶1的作用机制[J].第二军医大学学报，2016， 37（3）：295-301.
[12] 汤利华，方超，王浩然，等.山奈酚对高糖诱导的糖尿病肾病大鼠功能和组织病理损伤的保护作用[J].免疫学杂志，2018，34（12）：1041-1046.
[13] 靳英丽，屈智慧，杨盼盼，等.槲皮素对糖尿病大鼠肾脏足细胞nephrin和podocin表达的影响[J].中国实验诊断学，2019，23（3）：519-522.
[14] 付佳，黄潇红，黄静娟，等.槲皮素对糖尿病伤口的促愈合作用及相关机制研究[J].广东药科大学学报，2019， 35（3）：395-401.
[15] 罗维，艾磊，李显，等.慢性高糖抑制小鼠RAW264.7巨噬细胞AKT磷酸化并促进其M1型极化[J].细胞与分子免疫学杂志，2019，35（10）：910-917.
[16] 成光宇，丛婷婷，吕美香，等.解毒通络保肾法对高糖刺激下小鼠足细胞PI3K/Akt信号通路活化相关因子ILK、Akt及足细胞凋亡率的影响[J].中国老年学杂志，2019，39（5）：1174-1176.
[17] 杨佳慧，李香兰，徐晓华.血管内皮生长因子在糖尿病肾病中的研究进展[J].中国实验诊断学，2020，24（9）：1578-1581.
[18] Song W，Dang Q，Xu D，et al. Kaempferol induces cell cycle arrest andapoptosis in renal cell carcinoma through EGFR/p38 signaling [J]. Oncol Rep，2014，31（3）：1350-1356.
[19] 孙金鹏.肾脏中G蛋白偶联受体信号转导的多样性[J].中国药理学与毒理学杂志，2019，33（10）：825.
[20] 邱艳，陆灏.基于磷脂酰肌醇3-激酶/蛋白激酶B信号通路中医药治疗2型糖尿病研究进展[J].河北中医，2018，40（11）：1757-1760.
[21] 胡婷婷，罗向霞，张钰洁，等.磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路与糖尿病视网膜病变的关系研究进展[J].中医临床研究，2020， 12（29）：139-142.
[22] 孙明东，田鹤，于洋，等.小檗碱通过PI3K/Akt通路调控水通道蛋白-1表达改善糖尿病大鼠肾功能[J].解剖学杂志，2019，42（6）：561-564.
[23] 谭菲，盛瑶环，何勇.尿毒清颗粒对糖尿病肾病大鼠肾脏抗炎抗氧化保护作用及对TGF-β1/p38MAPK信号通路影响的研究[J].中药新药与临床药理，2019，30（1）：117-122.
[24] 张圭，杨溢，陆秀红.糖尿病肾病患者炎症因子的表达及与肾功能指标的相关性分析[J].中国当代医药，2019， 26（5）：67-69.
[25] 覃春美，李刚，程宗华，等.山楂叶总黄酮调控肾组织p38MAPK信号通路干预糖尿病肾病大鼠氧化应激损伤的实验研究[J].检验医学与临床，2019，16（21）：3131-3134.
[26] Guo MF，Dai YJ，Gao JR，et al. Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology [J]. Nucleic Acids Res，2018，46（1）：1074-1082.