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| Expression of transforming growth factor β in the liver and its biliary epithelial cells of rats with cholestatic liver disease |
| QIU Bingfeng XU Fangming XU Tangzhou XU Qi XI Jianwei |
| Department of Gastroenterology, Zhoushan Hospital of Zhoushan City, Zhejiang Province, Zhoushan 316000, China |
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Abstract [Abstract] Objective To explore the expression of transforming growth factor β (TGF-β) in the liver and its biliary epithelial cells of rats with cholestatic liver disease. Methods Sixty healthy female SPF SD rats aged 6-8 weeks were divided into control group (d0 group), 7 days after common bile duct ligation group (d7 group), 14 days after common bile duct ligation group (d14 group) and 21 days after common bile duct ligation group (d21 group), with 15 rats in each group, among which, the common bile duct of d0 group was touched softly after laparotomy and then abdomen was closed immediately. Knodell histological activity index (HAI) grading was used to evaluate the hepatic histologic changes of rats, the expression of TGF-β mRNA in liver tissue and biliary epithelial cells was detected by PCR, the amount of biliary epithelial cells within 4 d after cell culture was calculated by colorimetric method, the proliferative effect of TGF-β1 for biliary epithelial cells was observed. Results The Knodell HAI scores were improved with the extension of postoperative time (P ＜ 0.05); the levels of mRNA expression of TGF-β1, TGF-β2 and TGF-β3 were increased with the extension of cholestasis time (P ＜ 0.05); the study in vitro showed that TGF-β1 could inhibit the proliferation of biliary epithelial cells. Conclusion In the cholestatic liver disease caused by common bile duct ligation, the liver damage and expression of TGF-β mRNA are increased with the extension of cholestasis time, the level of TGF-β1 is closely related to the genesis and development of cholestatic liver disease, with the increase of the expression of TGF-β1 in hyperplastic biliary epithelial cells, the incidence of cholestatic hepatic fibrosis will be increased relevantly.
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