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| Effect of cyanidin-3-O-glucoside on JNK/FOXO1 of human primary osteoblasts |
| CHEN Lin1 HU Bosen1 ZHOU Bo1 CHEN Yong2 |
1.School of Public Health, Shenyang Medical College, Liaoning Province, Shenyang 110034, China;
2.Department of Orthopedics, Central Hospital Affiliated to Shenyang Medical College, Liaoning Province, Shenyang 110024, China |
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Abstract Objective To observe the effect of cyanidin-3-O-glucoside (C3G) on the proliferation of human primary osteoblasts and its effect on c-Jun N-terminal kinase/forkhead box O1 (JNK/FOXO1). Methods The cancellous bone tissues were collected from 60-90 years old patients with osteoporosis and femoral neck or intertrochanteric fracture who underwent artificial femoral head replacement or total hip replacement in Central Hospital Affiliated to Shenyang Medical College from June 2017 to June 2018, and osteoblasts were isolated and cultured in vitro. MTT assay was used to detect the proliferation activity of human primary osteoblasts in control group (α-MEM medium containing 0 μmol/L C3G), C3G group (α-MEM medium containing 100 μmol/L C3G). Real-time fluorescence quantitative PCR was used to detect mRNA expression of FOXO1, activiating transcripition factor-4 (ATF4), and osteoprotegerin (OPG) in control group and C3G group. Cells were treated with JNK pathway inhibitor (SP600125), and FOXO1 mRNA expression in SP600125-control group and SP600125-C3G group were detected by real-time fluorescence quantitative PCR. Results The proliferation rate of human primary osteoblasts in C3G group was higher than that in control group, and the difference was statistically significant (P < 0.05). The mRNA expression of FOXO1, ATF4, and OPG in C3G group were decreased than those in control group, and the differences were statistically significant (P < 0.05). There were no significant differences in the mRNA expression of FOXO1 between SP600125-control group and SP600125-C3G group (P > 0.05). Conclusion C3G may affect FOXO1 gene level through JNK pathway to promote human primary osteoblasts proliferation.
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