lncRNA SNHG3靶向调控miR-514a-5p对骨肉瘤细胞增殖、凋亡、迁移和侵袭的影响

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Abstract Objective To explore the effect of long non-coding RNA small nucleolar RNA host gene 3 (SNHG3) on the proliferation, apoptosis, migration and invasion of osteosarcoma cells and its mechanism. Methods The expression of SNHG3 and miR-514a-5p in osteoblasts hFOB1.19 and osteosarcoma cells SW-1353, HOS, U-2OS, and Saos-2 were detected by qRT-PCR. U-2OS cells in logarithmic growth phase were transfected. They were divided into si-NC group (transfected si-NC), si-SNHG3 group (transfected si-SNHG3), miR-NC group (transfected miR-NC), miR-514a-5p group (transfected miR-514a-5p), si-SNHG3+anti-miR-NC group (co-transfected si-SNHG3 and anti-miR-NC) and si-SNHG3 +anti-miR-514a-5p group (co-transfected with si-SNHG3 and anti-miR-514a-5p). CCK-8 assay and flow cytometry were used to check cell viability and apoptosis. The expression of c-Myc, Bax, and MMP-2 protein were detected by Western blot. Cell migration and invasion were tested by Transwell chamber experiment. The target relationship between SNHG3 and miR-514a-5p was analyzed by dual luciferase experiment. Results The expression of SNHG3 in osteosarcoma cells SW-1353, HOS, U-2OS, and Saos-2 were higher than those of osteoblasts hFOB1.19, and the expression of miR-514a-5p were lower than that of osteoblasts hFOB1.19, and the differences were statistically significant (P < 0.05). The SNHG3, cell viability, migrating cells, invasive cells, and the expression of c-Myc, MMP-2 protein in si-SNHG3 group were lower than those in si-NC group; while the apoptosis rate and the expression of Bax protein were higher than those in si-NC group, and the differences were statistically significant (P < 0.05). The miR-514a-5p expression, apoptosis rate, and the expression of Bax protein in miR-514a-5p group were higher than those in miR-NC group; while cell viability, migrating cells, invasive cells, the expression of c-Myc, MMP-2 protein were lower than those of miR-NC group, and the differences were statistically significant (P < 0.05). SNHG3 wild type luciferase activity of miR-514a-5p group was lower than that of miR-NC group, and the difference was statistically significant (P < 0.05), while SNHG3 mutant luciferase activity in miR-514a-5p group and miR-NC group showed no statistical significance (P > 0.05). The miR-514a-5p expression, apoptosis rate, and the expression of Bax protein in si-SNHG3+anti-miR-514a-5p group were lower than those in si-SNHG3+anti-miR-NC group, while cell viability, migrating cells, invaded cells, the expression of c-Myc and MMP-2 protein were higher than those in si-SNHG3+anti-miR-NC group, and the differences were statistically significant (P < 0.05). Conclusion Knockdown of SNHG3 can significantly inhibit the proliferation, invasion and migration of osteosarcoma cells, and also promote the apoptosis of osteosarcoma cells, the mechanism of which may be related to miR-514a-5p.

Key words： Osteosarcoma cells Proliferation Apoptosis Migration Invasion

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	TAN Xiaoqian MEI Haibo WU Jiangyan YAN An

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TAN Xiaoqian MEI Haibo WU Jiangyan YAN An. Effect of lncRNA SNHG3 on the proliferation, apoptosis, migration and invasion of osteosarcoma cells by targeting miR-514a-5p[J]. 中国医药导报, 2021, 18(22): 22-27.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I22/22

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