Abstract Objective To observe the protective effect of triptolide on retinal ganglion cells (RGCs) in rats with acute ocular hypertension. Methods Sixty Wister rats were selected, male and female in half. The rats were divided into control group, model group and Triptolide group by random number table method, with 20 rats in each group. Model group and Triptolide group were modeled by anterior chamber perfusion and pressure method. After successful modeling, the Triptolide group was intraperitoneally injected with 10 μg/kg of Triptolide daily, while the control group and model group were intraperitoneally injected with the same amount of normal saline daily, for a total of seven days. The pathological changes of retinal layers were observed by HE staining. The apoptosis of RGCs was observed by TUNEL staining and the survival was observed by immunofluorescence method. The expression of caspase-3 in the retinas of the three groups was detected by immunohistochemistry. Results In the control group, the retinal layers were arranged neatly and the nuclear boundaries were clear. The retinal layers of the model group were irregular and atrophied, while the retinal layers of the triptolide group were clear and orderly. The thickness of retinal nerve fiber layer (RNFL), the number of apoptotic RGCs and the number of caspase-3 positive cells in model group were higher than those in control group, and the number of survival RGCs was lower than that in control group, with statistical significance (all P ＜ 0.05). The thickness of RNFL, number of apoptotic RGCs and number of caspase-3 positive cells in triptolide group were lower than those in model group, and the number of survival RGCs was higher than that in model group, with statistical significance (all P ＜ 0.05). Conclusion Triptolide has a significant protective effect on RGCs in rats with acute ocular hypertension.
WANG Xinfang MA Teng. Protective effect of triptolide on retinal ganglion cells in rats with acute ocular hypertension[J]. 中国医药导报, 2021, 18(18): 13-16.
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