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| Exploring mechanism of Astragali Radix in the treatment of bronchial asthma on network pharmacology |
| LI Zhuying1 CHEN Lu2 YUAN Xingxing2 WANG Ting3 ZHANG Yu2 LIAN Mengyao2 LI Xing1 |
1.Department of Respiratory, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Graduate School, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.Institute of Pathology, Qiqihar Medical University, Heilongjiang Province, Qiqihar 161000, China |
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Abstract Objective To explore the possible mechanism of Astragali Radix in the treatment of bronchial asthma by network pharmacology. Methods The effective chemical ingredients and corresponding targets of Astragali Radix were obtained based on traditional Chinese medicine systems pharmacology database and analysis platform. The targets related to bronchial asthma were obtained through Genecards database, and the potential targets of Astragali Radix acting on bronchial asthma were obtained by mapping Astragali Radix targets and disease targets. Astragali Radix-compound-target-disease network was constructed by Cytoscape software. Proteinprotein interaction (PPI) data were obtained from the STRING online database and imported into Cytoscape to construct PPI network. The gene ontology (GO) function enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis of the targets was carried out using the Bioconductor bioinformatics software package. Results Nineteen active compounds of Astragali Radix were screened and obtained. Ninety-seven predicted targets of active compounds of Astragali Radix were obtained. A total of 5973 targets related to bronchial asthma were found in Genecards database. A total of 81 potential targets of Astragali Radix on bronchial asthma were obtained. The results of GO and KEGG enrichment analysis showed that DNA and RNA transcription regulation, activity and receptor binding of cytokines and transcription factors, anti-oxidant stress, hormone regulation, protein binding, enzyme activity and other biological processes were mainly involved by adjusting AGE-RAGE signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, p53 signaling pathway and other pathways to exert its treatment of bronchial asthma. Conclusion Astragali Radix and its active ingredients exert anti-inflammatory, anti-oxidation, immune regulation and other effects through various targets and multiple pathways, and play a therapeutic role in inflammation, airway hyperresponsiveness and airway remodeling caused by bronchial asthma.
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