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| Expression and clinopathological significance of UBD in Kazakh, Uyghur and Han esophageal squamous cell carcinoma |
| LUO Xiaolei1,2 KAWULI Jumai1,2 SUN Qingchao1,2 LI Desheng1,2 MADINIYAT Niyaz2,3 ZHANG Liwei1,2 YILIYAER Xiaheding1,2 |
1.Department of Thoracic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China; 2.Xinjiang Esophgeal Cancer Research Institute, Xinjiang Uygur Autonomous Region, Urumqi 830054, China;
3.Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China |
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Abstract Objective To investigate the expression of protein and mRNA levels of Ubiquitin D (UBD) with cancer tissues and adjacent tissues in esophageal squamous cell carcinoma patients in Kazakh, Uygur, and Han nationalities, and to explore its clinicopathological significance. Methods A total of 173 cases of esophageal squamous cell carcinoma patients with cancer tissue and adjacent tissue specimens were collected (normal esophageal mucosa tissue> 5 cm from cancer tissue) from 2011 to 2014, including Uyghur, Kazakh and Han nationalities. Immunohistochemical staining method, real-time fluorescent quantitative PCR and HE staining method were used to detect the expression level of UBD, and the clinicalpathological characteristics of patients were combined for analysis. Results The protein and mRNA levels of UBD in esophageal squamous cell carcinoma tissues of the three ethnic groups were higher than those of adjacent tissues, and the differences were statistically significant (P ＜ 0.05). In the esophageal squamous cell carcinoma tissues of different ethnic groups, there was no significant difference in the expression of UBD at the protein and mRNA levels (P ＞ 0.05). UBD expression in esophageal squamous cell carcinoma tissues was related to tumor neuro vascular invasion, TNM stage, N stage and differentiation degree (P ＜ 0.05), and it wasn’t related to gender, age, T stage, tumor location, tumor size, etc.(P ＞ 0.05). Conclusion UBD may have an important influence on the malignant development of esophageal squamous cell carcinoma, and it is expected to become a reference indicator for predicting the potential of metastasis.
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