针刺捻转补泻法对自发性高血压大鼠海马丙二醛、谷胱甘肽过氧化物酶含量的影响

Abstract
Figure/Table
References (34)
Related Citation (15)

Download: PDF (794 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in hippocampus of spontaneously hypertensive rats. Methods A total of 18 spontaneously hypertensive rats were divided into three groups by random number table method: reducing method group, reinforcing method group, and model group, with six rats in each group. Six WKY rats were served as blank group. All rats were SPF grade, nine-week-old male rats. After one week of acclimatization, reducing method group and reinforcing method group were intervened with corresponding manipulation of reducing and reinforcing on bilateral tàichōng once a day for 14 days. Model group and blank control group received the same grasping and fixation stimulation at the same points of reinducing method group and reinforcing method group. Hippocampus samples were collected on the 15th day, and the morphology of hippocampus was observed by HE staining, MDA content was detected by thiobarbituric acid method and GSH-Px content was detected by colorimetry. Results HE staining results showed that the cells in the blank group had complete structure, regular morphology, normal intercellular space, most nuclei were deeply stained, nucleoli were clear, and no denatured necrosis was observed. Compared with the blank group, the staining in the model group was significantly lighter, and nuclear pyknosis, cell lysis and fragmentation, vacuolar degeneration and apoptotic cells were observed. Compared with the model group, the cell contour was clear, the cell staining was deep, the nucleoli were clear, and the cell structure was basically normal in both the reducing method group and reinforcing method group. MDA content in model group was higher than that in blank group, while GSH-Px content in model group was lower than that in blank group, and the differences were highly statistically significant (P ＜ 0.01). MDA contents in reducing method group and reinforcing method group were lower than those in model group, the contents of GSH-Px in the reducing method group and reinforcing method group were higher than those in the model group, and the differences were statistically significant (P < 0.05 or P ＜ 0.01). Conclusion Reinforcing-reducing method by twirling of acupuncture can regulate the expression level of MDA and GSH-Px in the hippocampus of spontaneously hypertensive rats, inhibit the oxidative reaction, and reduce the damage of oxygen radicals to the hippocampus of spontaneously hypertensive rats, which may be one of the protective mechanisms of reinforcing-reducing method by twirling of acupuncture on hypertensive hippocampal injury.

Key words： Acupuncture Reinforcing-reducing method by twirling Hypertension Hippocampus Malondialdehyde Glutathione peroxidase

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XUE Yanjun WU Jiaojuan ZHANG Xudong YU Pengcheng WU Xiaoli LIU Qingguo

Cite this article:

XUE Yanjun WU Jiaojuan ZHANG Xudong YU Pengcheng WU Xiaoli LIU Qingguo. Effects of reinforcing-reducing method by twirling of acupuncture on contents of malondialdehyde and glutathione peroxidase in hippocampus of spontaneously hypertensive rats[J]. 中国医药导报, 2021, 18(17): 4-7,17.

URL:

https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I17/4

[1] Mills KT，Stefanescu A，He J. The global epidemiology of hypertension [J]. Nat Rev Nephrol，2020，16（4）：223-237.
[2] 严心，陈晓平.高血压前期人群心血管病及靶器官损害的研究进展[J].中华高血压杂志，2019，27（5）：483-488.
[3] 中国高血压防治指南修订委员会，高血压联盟（中国），中华医学会心血管病学分会，等.中国高血压防治指南（2018年修订版）[J].中国心血管杂志，2019，24（1）：24-56.
[4] 徐萧洪，王舒.针灸太冲穴对治疗高血压病的研究进展[J].现代医学与健康研究电子杂志，2019，3（15）：1-3.
[5] 熊飞然，王舒.针刺太冲穴治疗高血压病研究概况[J].辽宁中医药大学学报，2019，21（4）：221-224.
[6] 李玉龙，贺军.针刺捻转补泻手法的研究进展[J].中华针灸电子杂志，2020，9（3）：109-111.
[7] 曾天笑，梁靖蓉，孙娇，等.针刺捻转补泻手法对自发性高血压大鼠顶叶皮质IL-6、TNF-α及NGF影响的研究[J].中国中医急症，2020，29（8）：1335-1338.
[8] 郝晓敏，梁靖蓉，曾天笑，等.针刺捻转补泻手法对自发性高血压大鼠小脑GABA、Glu表达的影响[J].中国中医急症，2020，29（4）：584-588.
[9] 郭秋蕾，刘清国，贾文睿，等.针刺捻转补泻手法对SHR下丘脑RAS的影响及其中枢机制[J].中华中医药杂志，2018，33（11）：4920-4924.
[10] 孙启胜，郭骐影，贾文睿，等.针刺捻转泻法对应激性高血压前期大鼠下丘脑基因表达谱的影响[J].针刺研究，2017，42（3）：209-216.
[11] 战河，王赫，杨芳媛，等.针刺捻转补泻手法对自发性高血压大鼠顶叶皮质NO、5HT的影响[J].中国中医急症，2018，27（3）：377-379，383.
[12] 王赫，杨芳媛，战河，等.针刺捻转补泻法对自发性高血压大鼠小脑NE、5-HT及NO的影响[J].环球中医药，2018，11（3）：353-357.
[13] Poulet R，Gentile MT，Vecchione C，et al. Acute hypertension induces oxidative stress in brain tissues[J].J Cereb Blood Flow Metab，2006，26（2）：253-262.
[14] 王婧，牛舰霏，李冠会，等.针刺治疗原发性高血压病机制的研究概况[J].中国针灸，2019，39（2）：224-228.
[15] 孙嫘，郑婕，徐建超，等.针刺人迎穴对自发性高血压大鼠血压及延髓头端腹外侧区氧化应激反应的影响[J].中医药导报，2019，25（2）：101-104.
[16] 林文注，王佩.实验针灸学[M].上海：上海科学技术出版社，1999：55.
[17] 李正欢，张晓云.高血压动物模型应用概况与研究进展[J].中华高血压杂志，2017，25（8）：727-732.
[18] 卢文丽，方肇勤，潘志强，等.SHR、DOCA-盐及肾性高血压大鼠的证候特征及比较[J].四川中医，2008（11）：25-28.
[19] 刘盼利.针灸治疗高血压病的选穴规律文献研究[D].沈阳：辽宁中医药大学，2017.
[20] 卢永屹，曾科.针灸治疗原发性高血压选穴规律的研究[J].针灸临床杂志，2012，28（10）：48-50.
[21] 刘海华，王莹莹，高海波，等.针灸治疗高血压病选穴规律文献研究[J].中医杂志，2014，55（12）：1055-1058.
[22] 孙娇，梁靖蓉，曾天笑，等.针刺捻转补泻手法对自发性高血压大鼠海马-HPA轴的影响及其降压机制[J].针灸临床杂志，2020，36（5）：67-70.
[23] Hirooka Y，Sagara Y，Kishi T，et al. Oxidative stress and central cardiovascular regulation[J].Circ J，2010，74（5）：827-835.
[24] 王瑞钰，薛倩，黄晶.氧化应激与压力感受器敏感性在高血压治疗中的研究进展[J].心血管病学进展，2019， 40（1）：45-48.
[25] 毛文星，李冰，王志梅，等.氧化应激、血管内皮功能障碍与高血压[J].现代生物医学进展，2014，14（19）：3770-3774.
[26] Shih YH，Tsai SF，Huang SH，et al. Hypertension impairs hippocampus-related adult neurogenesis，CA1 neuron dendritic arborization and long-term memory [J]. Neuroscience，2016，322（2）：346-357.
[27] Glodzik L，Rusinek H，Tsui W，et al. Different Relationship Between Systolic Blood Pressure and Cerebral Perfusion in Subjects with and Without Hypertension [J]. Hypertension，2019，73（1）：197-205.
[28] Tucsek Z，Noa Valcarcel-Ares M，Tarantini S，et al. Hypertension-induced synapse loss and impairment in synaptic plasticity in the mouse hippocampus mimics the aging phenotype：implications for the pathogenesis of vascular cognitive impairment [J]. Geroscience，2017，39（4）：385-406.
[29] 袁征.基于核磁共振技术（1H-NMR）的高血压病肝阳上亢证代谢模式及平肝方药的干预研究[D].济南：山东中医药大学，2012.
[30] 刘大洋.临床生化指标与氧化应激标志物与高血压患病风险的关联性研究[D].武汉：华中科技大学，2019.
[31] 宁思思，杨绍政，马家怡，等.电针对自发性高血压大鼠肾脏Klotho蛋白表达及氧化应激的影响[J].中华中医药杂志，2019，34（8）：3739-3742.
[32] 纪智，袁静云，王紫娟，等.针刺对自发性高血压大鼠心肌ANP、ACE2表达和Ang（1-7）/AngⅡ比值的影响[J].中国医药导报，2019，16（17）：4-8.
[33] 万基伟，樊小农，王舒，等.氧化应激与高血压发病机制研究[J].中医学报，2015，30（1）：101-104.
[34] 刘健，杨沙，樊小农，等.基于氧化/抗氧化酶系统的针刺人迎穴干预自发性高血压大鼠的氧化应激研究[Z].天津：天津中医药大学第一附属医院，2018-05-31.