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Relationship between intestinal flora disorder and Th17/Treg balance and related cytokines in patients with ulcerative colitis |
YE Jia ZHU Meiping LIU Chang |
Department of Spleen and Stomach Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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Abstract Objective To investigate the relationship between intestinal flora disorder and helper T cell 17 (Th17)/regulatory T cell (Treg) balance and related cytokines in patients with ulcerative colitis (UC). Methods From July 2018 to July 2020, 30 patients with active UC (AUC) admitted to Shuguang Hospital, Shanghai University of Traditional Chinese Medicine (hereinafter referred to as “our hospital”) were selected as the AUC group, and 30 patients with remission UC (RUC) in our hospital were selected as the RUC group with a ratio of 1∶1. Another 30 healthy subjects who came to our hospital for physical examination during the same period were selected as the control group. Real-time quantitative polymerase chain reaction was used to detect the number of intestinal flora in the three groups, the percentage of Th17 and Treg cells in peripheral blood was determined by flow cytometry, and the expression of inflammatory factors in colonic mucosa was determined by immunohistochemistry. Pearson correlation was used to analyze the correlation between the expression levels of Th17, Treg and inflammatory factors and the number of intestinal flora. Results The number of Enterococcus and Enterobacter, the proportion of Th17 cells in peripheral blood and the expression of interleukin (IL)-2, IL-6 and IL-17 in mucosa tissues in AUC group were significantly higher than those in RUC group and control group, and RUC group was higher than control group (P < 0.05); the number of Bacteroides, Lactobacillus and Bifidobacterium, the proportion of Treg cells in peripheral blood, the Th17/Treg ratio and the expression of IL-10 in mucosal tissues in AUC group were significantly lower than those in RUC group and control group, and RUC group was lower than control group (P < 0.05). Pearson correlation analysis showed that Th17, IL-2, IL-6 and IL-17 were positively correlated with Enterococcus and Enterobacter, it was negatively correlated with Bacteroidetes, Lactobacillus and Bifidobacterium. Treg and IL-10 were negatively correlated with Enterococcus and Enterobacterium, and positively correlated with Bacteroidetes, Lactobacillus and Bifidobacterium (all P < 0.05). Conclusion The disturbance of intestinal flora may affect the immune balance of Th17/Treg through various mechanisms, regulate the secretion of inflammatory mediators, and participate in the occurrence and development of UC.
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