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| Effect of allergic reaction induced by echinococcosis granulosa on intestinal mucosal barrier function |
| SHANG Haibo LI Xin LIU Keke WANG Jiang▲ |
| Department of Anesthesiology, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China |
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Abstract Objective To investigate the effect of allergic reaction induced by echinococcosis granulosa on intestinal mucosal barrier function. Methods Forty-five female BALB/c mice aged six to eight weeks with SPF grade and body weight of 18 to 22 g were divided into blank control group, echinococcosis granulosa model group and echinococcosis granulosa sensitization group by random number table method, with 15 mice in each group. Echinococcus granulosus microcapsules were intraperitoneally injected into mice of echinococcosis granulosa model group and echinococcosis granulosa sensitization group. Each mouse was inoculated with about 50 microcapsules to establish the hydatid disease mouse model. After feeding for three months, the echinococcosis granulosa sensitization group was injected with crude hydatid cyst fluid to induce allergic reaction, while the blank control group and the echinococcosis granulosa model group were injected with the same amount of normal saline. The contents of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and phosphorylated nuclear factor of kappa B-p65 (p-NF-κBp65), diamine oxidase (DAO) and D-lactic acid in plasma and the apoptosis rate of intestinal mucosal epithelial cells were detected in intestinal tissue of mice in each group. Pathological sections were performed to evaluate the barrier function. Results Compared with echinococcosis granulosa model group, the contents of TNF-α, IL-6, p-NF-κBp65, and the contents of DAO and D-lactic acid in plasma were increased in echinococcosis granulosa sensitization group (P ＜ 0.05). Compared with echinococcosis granulosa model group, intestinal mucosa of echinococcosis granulosa sensitization group was significantly atrophied, part of intestinal villi became shorter or broke off, more acute and chronic inflammatory cells were infiltrated in the interstitium, and intestinal mucosa epithelial cells apoptosis increased. Conclusion Allergic reaction induced by echinococcosis granulosa can cause intestinal mucosal injury and lead to intestinal mucosal barrier dysfunction.
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