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| Discussion on the molecular mechanism of “Lonicerae Japonicae Flos and Scrophulariae Radix” in the treatment of atherosclerosis based on network pharmacology |
| WANG Shuqi1，2 SUN Kehan1 CHEN Fei3 GAO Zhao2 LIN Yiyi2 YANG Manfang1 XU Xinrui2 NIE Bo1，2 |
1.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
2.Key Laboratory of Chinese Internal Medicine of Ministry of Education, Dongzhimen Hospital, Beijing University of Chinese Medicine Beijing Key Laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
3.Logistics Support, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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Abstract Objective To explore the active ingredients and potential molecular mechanism of “Lonicerae Japonicae Flos and Scrophulariae Radix” in the treatment of atherosclerosis (AS) based on network pharmacology. Methods The pharmacology database and analysis platform of traditional Chinese medicine system (TCMSP) was used and the related literatures at home and abroad were consulted. Combined with the previous experimental research, all the active ingredients and targets related to Lonicerae Japonicae Flos and Scrophulariae Radix were found. AS related targets in DisGeNET, OMIM, GeneCards were searched. The intersection targets of drug targets and disease targets were uploaded to the String platform, and the PPI network diagram was drawn by using Cytoscape software. David database was used to analyze the GO biological process and KEGG pathway enrichment of the core target protein, and to construct the network diagram of “Lonicerae Japonicae Flos and Scrophulariae Radix”-AS disease target-signal pathway. Results Through database analysis and literature review, a total of 37 active components, 231 potential drug targets, 4410 disease targets, 173 drug and disease cross target proteins, and “Lonicerae Japonicae Flos and Scrophulariae Radix” interferes with 29 core target proteins of AS were obtained. GO bioprocess analysis identified 49 items according to the screening requirements. KEGG enrichment results showed that there were 55 pathways related to AS, including tumor necrosis factor signaling pathway, Toll like receptor signaling pathway, mitogen activated protein kinase signaling pathway, etc. Conclusion It is necessary to explore the potential molecular mechanism of “Lonicerae Japonicae Flos and Scrophulariae Radix” compatibility against AS from a holistic perspective, and to explore the research mode of “multi-component of traditional Chinese medicine/bioinformatics/multi-target multi-channel action/experimental verification”, so as to provide a fast and accurate research strategy for elucidating the effective substances and mechanism of traditional Chinese medicine.
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