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| Effect of Osimertinib on blood-cord barrier permeability after spinal cord injury in rats |
| LI Zaiwang1 CAO Tingting2 WANG Qian1 TU Jingmei1 HAN Jing1 |
1.Department of Neurology, the Second Clinical Medical College of Ji’nan University the First Affiliated Hospital of Southern University of Science and Technology Shenzhen People’s Hospital, Guangdong Province, Shenzhen 518020, China;
2.Department of Neurology, Yancheng First People’s Hospital, Jiangsu Province, Yancheng 224001, China |
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Abstract Objective To investigate the effects of epidermal growth factor receptor (EGFR) inhibitor Osimertinib on blood-cord barrier permeability after spinal cord injury (SCI) in rats. Methods A total of sixty eight week-old female SD rats (200 to 250 g) were divided into sham operation group (n = 20) and SCI model group (n = 40) according to random number table method. The 40 SCI model rats were divided into injury group and Osimertinib group by random number table method, with 20 rats in each group. The Osimertinib group was gavaged with 0.4 mg/mL Osimertinib solution (2 mL/d), and the sham operation group and the injury group were gavaged with normal saline (2 mL/d). All the three groups were treated for 3 days. The water content of spinal cord tissue was determined 3 days after SCI, the expression of aquaporin-4 (AQP-4) protein was detected by Western blot, the evans blue (EB) dye leakage test and the albumin content of spinal cord were determined by immunofluorescence technique to evaluate the changes of blood and spinal cord barrier permeability. Basso-Beattie-Bresnahan (BBB) score was used to evaluate the motor function of rats. Results The water content of spinal cord in Osimertinib group at 3 days after SCI was significantly lower than that in injury group (P ＜ 0.05). The expression of AQP-4 protein in Osimertinib group was significantly lower than that in injury group (P ＜ 0.01). The concentration of EB dye in spinal cord tissue in Osimertinib group was less than that in injury group, and the fluorescence intensity of EB was weaker than that in injury group (P ＜ 0.05). The immunofluorescence intensity of albumin in Osimertinib group was weaker than that in injury group. The overall BBB score of Osimertinib group was significantly higher than that of injury group (P ＜ 0.05). Conclusion The EGFR inhibitor Osimertinib can improve the damage of the blood-cord barrier after SCI and alleviate the secondary injury of the spinal cord, and promote the recovery of neurological function.
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