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| Study on the difference of aporphine alkaloids in Radix linderae before and after processing methods by UPLC-QTOF-MS/MS |
| SHE Bo1 XIANG Xingliang1 SHI Qingxin1 JIN Shu’na2 ZHANG Lijun2 SONG Chengwu1 SU Chao1▲ HUANG Rongzeng1▲ |
1.College of Pharmacy, Hubei University of Chinese Medicine, Hubei Province, Wuhan 430065, China;
2.College of Basic Medicine, Hubei University of Chinese Medicine, Hubei Province, Wuhan 430065, China |
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Abstract Objective To identify and characterize aporphine alkaloids in Radix linderae by UPLC-QTOF-MS/MS, and to investigate the effect of different processing methods on such compounds. Methods Waters ACQUITY BEH C18 column (1.7 μm, 2.1 mm×100 mm) was performed, column temperature was 40℃, and mobile phase was methanol-water with gradient elution. Electrospray ion source and MSE (full information tandem mass spectrometry) scanning in positive ion mode were used. The aporphine alkaloids in raw and processed products were analyzed. Results A total of 36 kinds of aporphine alkaloids were identified. Compared with the raw, the relative contents of aporphine alkaloids decreased by 18 and increased by 9 after frying; the relative content decreased by 21 and increased by 6 after treated with vinegar; the relative content decreased by 21 and increased by 6 after wine treatment; the relative content decreased by 23 and increased by 7 after salting. Conclusion After processing, the content of most aporphine alkaloids has changed significantly, which can provide the material basis for the effect change of Radix linderae.
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