基底刚度对细胞生物学行为作用的研究进展

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Abstract Stiffness is an important parameter of micro nano mechanics, which can be divided into cell stiffness and substrate stiffness. Substrate stiffness can be understood as the elastic modulus or hardness of tissue, which provides support and specific physical signals for cell growth. With the development of atomic force microscope, the substrate stiffness can be measured at present. Substrate stiffness plays an important role in many biological behaviors of cells, such as cell morphology, cell migration, cell phenotype and function, and regulation of stem cell differentiation. These phenomena exist in both normal cells and tumor cells, and have different meanings. In tumor tissue, substrate stiffness may also affect the invasiveness of tumor and the sensitivity of anti-tumor drugs. Understanding the mechanism of these phenomena may provide new ideas for the prognosis and treatment of tumors.

Key words： Substrate stiffness Cell proliferation Cell migration Cell differentiation

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	DENG Bo’er KONG Weimin

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DENG Bo’er KONG Weimin. Research progress on the effect of substrate stiffness on cell biological behavior[J]. 中国医药导报, 2021, 18(12): 57-60.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I12/57

[1] Wells RG. The role of matrix stiffness in regulating cell behavior [J]. Hepatology，2008，47（4）：1394-1400.
[2] Eble JA，Niland S. The extracellular matrix in tumor progression and metastasis [J]. Clin Exp Metastasis，2019，36（3）：171-198. doi：10.1007/s10585-019-09966-1.
[3] Mollaeian K，Liu Y，Bi S，et al. Nonlinear Cellular Mechanical Behavior Adaptation to Substrate Mechanics Identified by Atomic Force Microscope [J]. Int J Mol Sci，2018，19（11）：3461.
[4] Agache PG，Monneur C，Leveque JL，et al. Mechanical properties and Young’s modulus of human skin in vivo [J]. Arch Dermatol Res，1980，269（3）：221-232.
[5] Pailler-Mattei C，Bec S，Zahouani H. In vivo measurements of the elastic mechanical properties of human skin by indentation tests [J]. Med Eng Phys，2008，30（5）：599-606.
[6] Budday S，Nay R，de Rooij R，et al. Mechanical properties of gray and white matter brain tissue by indentation [J]. J Mech Behav Biomed Mater，2015，46：318-330.
[7] Moreno-Layseca P，Icha J，Hamidi H，et al. Integrin trafficking in cells and tissues [J]. Nat Cell Biol，2019，21（2）：122-132.
[8] De Franceschi N，Hamidi H，Alanko J，et al. Integrin traffic-the update [J]. J Cell Sci，2015，128（5）：839-852.
[9] Melica ME，La Regina G，Parri M，et al. Substrate Stiffness Modulates Renal Progenitor Cell Properties via a ROCK-Mediated Mechanotransduction Mechanism [J]. Cells，2019，8（12）：1561.
[10] 卢方理，贝筝，杨飞燕，等.基底/细胞系统刚度对人脐静脉内皮细胞增殖、凋亡及形态的影响[J].湖南师范大学学报，2019，16（5）：11-15.
[11] Wu Y，Xiang Y，Fang J，et al. The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells [J]. Biosci Rep，2019，39（1）：BSR20181748.
[12] Gouveia RM，Vajda F，Wibowo JA，et al. YAP，ΔNp63，and β-Catenin Signaling Pathways Are Involved in the Modulation of Corneal Epithelial Stem Cell Phenotype Induced by Substrate Stiffness [J]. Cells，2019，8（4）：347. doi：10.3390/cells8040347.
[13] Xie J，Zhang D，Zhou C，et al. Substrate elasticity regulates adipose-derived stromal cell differentiation towards osteogenesis and adipogenesis through β-catenin transduction [J]. Acta Biomater，2018，79：83-95.
[14] Liu B，Kilpatrick JI，Lukasz B，et al. Increased Substrate Stiffness Elicits a Myofibroblastic Phenotype in Human Lamina Cribrosa Cells [J]. Invest Ophthalmol Vis Sci，2018，59（2）：803-814.
[15] Okamoto T，Takagi Y，Kawamoto E，et al. Reduced substrate stiffness promotes M2-like macrophage activation and enhances peroxisome proliferator-activated receptor γ expression [J]. Exp Cell Res，2018，367（2）：264-273.
[16] Mennens SFB，Bolomini-Vittori M，Weiden J，et al. Substrate stiffness influences phenotype and function of human antigen-presenting dendritic cells [J]. Sci Rep，2017， 7（1）：17511.
[17] Zeng Y，Yi J，Wan Z，et al. Substrate stiffness regulates B-cell activation，proliferation，class switch，and T-cell-independent antibody responses in vivo [J]. Eur J Immunol，2015，45（6）：1621-1634.
[18] Wen YQ，Gao X，Wang A，et al. Substrate stiffness affects neural network activity in an extracellular matrix proteins dependent manner [J]. Colloids Surf B Biointerfaces，2018，170：729-735.
[19] Nguyen DT，Nagarajan N，Zorlutuna P. Effect of Substrate Stiffness on Mechanical Coupling and Force Propagation at the Infarct Boundary [J]. Biophys J，2018，115（10）：1966-1980.
[20] Liu H，Paul C，Xu M. Optimal Environmental Stiffness for Stem Cell Mediated Ischemic Myocardium Repair [J]. Methods Mol Biol，2017，1553：293-304.
[21] Mu?觡oz A，Eldridge WJ，Jakobsen NM，et al. Cellular shear stiffness reflects progression of arsenic-induced transformation during G1 [J]. Carcinogenesis，2018，39（2）：109-117.
[22] 赵轩宇，焦思萌，宋丹，等.基底刚度对人宫颈癌HeLa细胞生长影响的体外研究[J].癌症进展，2018，16（2）：155-158.
[23] Peng Y，Chen Z，Chen Y，et al. ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness [J]. Acta Biomater，2019，88：86-101.
[24] Hamidi H，Ivaska J. Every step of the way：integrins in cancer progression and metastasis [J]. Nat Rev Cancer，2018，18（9）：533-548. doi：10.1038/s41568-018-0038-z.
[25] Shukla VC，Higuita-Castro N，Nana-Sinkam P，et al. Substrate stiffness modulates lung cancer cell migration but not epithelial to mesenchymal transition [J]. J Biomed Mater Res A，2016，104（5）：1182-1193.
[26] Zhao D，Li Q，Liu M，et al. Substrate stiffness regulated migration and invasion ability of adenoid cystic carcinoma cells via RhoA/ROCK pathway [J]. Cell Prolif，2018， 51（3）：e12442.
[27] Feng J，Tang Y，Xu Y，et al. Substrate stiffness influences the outcome of antitumor drug screening in vitro [J]. Clin Hemorheol Microcirc，2013，55（1）：121-131. doi：10.3233/CH-131696.
[28] 焦思萌，赵轩宇，宋丹，等.基底刚度对人宫颈癌HeLa细胞增殖及顺铂药物敏感性影响的体外研究[J].癌症进展，2019，17（22）：2642-2644，2651.
[29] Luo Q，Kuang D，Zhang B，et al. Cell stiffness determined by atomic force microscopy and its correlation with cell motility [J]. Biochim Biophys Acta，2016，1860（9）：1953-1960.
[30] Chiang MY，Yangben Y，Lin NJ，et al. Relationships among cell morphology，intrinsic cell stiffness and cell-substrate interactions [J]. Biomaterials，2013，34（38）：9754-9762.
[31] Medina SH，Bush B，Cam M，et al. Identification of a mechanogenetic link between substrate stiffness and chemotherapeutic response in breast cancer [J]. Biomaterials，2019，202：1-11.
[32] Miyazawa A，Ito S，Asano S，et al. Regulation of PD-L1 expression by matrix stiffness in lung cancer cells [J]. Biochem Biophys Res Commun，2018，495（3）：2344-2349. doi：10.1016/j.bbrc.2017.12.115.
[33] Piao J，You K，Guo Y，et al. Substrate stiffness affects epithelial-mesenchymal transition of cervical cancer cells through miR-106b and its target protein DAB2 [J]. Int J Oncol，2017，50（6）：2033-2042.
[34] Zhao D，Xue C，Li Q，et al. Substrate stiffness regulated migration and angiogenesis potential of A549 cells and HUVECs [J]. J Cell Physiol，2018，233（4）：3407-3417.