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| Study on the mechanism of new type of topical anesthetic in oral mucosal anesthesia of New Zealand rabbits |
| ZENG Dong1 NIU Jiao2 LI Changyi3 CHANG Jin4 NIU Guangliang1▲ |
1.Department of Dentistry, Bejing Hospital of Integrated Traditional Chinese and Western Medicine, Beijing 100039, China;
2.Department of Dentistry, the Second Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China;
3.Department of Hospital Office, Tianjin Medical University, Tianjin 300070, China;
4.Institute of Nanomedicine Tianjin University School of Life Sciences, Tianjin 300072, China |
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Abstract Objective To investigate the mechanism of oral mucosal surface anesthesia with new type of oral topical anesthetic which as Lidocaine hydrochloride lysine-linoleic acid modified dextran polymeric lipid vesicles (LID-LLD-PLVs). Methods Twenty-four New Zealand rabbits aged eight months were randomly divided into four groups randomly according to body weight layer according to the random number table method, and six in each group, which were blank control group (no administration), 0.5 mL LID-LLD-PLVs were applied on the gingival surface of mandibular anterior teeth, then the group was divided into LD-LD-PLVS 30 min, 60 min and 90 min groups according to the action time. Then the anterior mucosa of each group was injected with 25 μL of 10% formalin solution for 60 min, and the gingiva, ipsilateral trigeminal ganglion and medulla oblongata were extracted. Real-time PCR was used to quantitatively analyze the mRNAs of substance P (SP), calcitonin gene related peptide (CGRP) and c-fos in gingival, trigeminal ganglion and medulla oblongata. Results The mRNA quantitative of SP, CGRP and c-fos in the gingival, trigeminal ganglion and medulla oblongata of LID-LLD-PLVs 30 min, 60 min and 90 min groups were all lower than that of the control group, and the differences were statistically significant (all P ＜ 0.05). Conclusion The mechanism of LID-LLD-PLVs may be related to the inhibition of the expression levels of SP, CGRP and c-fos in gingival, trigeminal ganglion and medulla oblongata.
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