透明细胞肾细胞癌组织中miR-4313、TBX15的表达及临床意义

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Abstract Objective To study the expression and clinical significance of microRNA (miR)-4313 and T-box transcription factor 15 (TBX15) in clear cell renal cell carcinoma (ccRCC). Methods Fluorescence quantitative PCR was used to detect the mRNA expression of miR-4313 and TBX15 in the cancer tissues of 93 ccRCC patients treated in Benxi Central Hospital, Liaoning Province from March 2018 to December 2019. Immunohistochemistry was used to detect the expression of TBX15 protein in the cancer tissues. Normal tissues adjacent to the cancer were used as control. The relationship between miR-4313 and TBX15 protein expression in cancer tissues and clinicopathological parameters was analyzed. The correlation between miR-4313 and TBX15 mRNA expression were analyzed by Pearson linear correlation analysis. Results Compared with adjacent tissues, the relative expression level of miR-4313 in ccRCC cancer tissue was significantly lower, while the relative expression level of TBX15 mRNA was significantly higher, and the differences were statistically significant (all P ＜ 0.05). The positive expression rate of TBX15 protein in cancer tissues was significantly higher than that in adjacent tissues, and the difference was statistically significant (P ＜ 0.05). There was no statistical significance in the expression of miR-4313 and TBX15 protein in cancer tissues among patients with different ages, genders, pathological grades, distant metastasis and lymph node metastasis (P ＞ 0.05). The miR-4313 expression in tumor stage Ⅲ-Ⅳ ccRCC cancer tissues were significantly lower than stage Ⅰ-Ⅱ, the TBX15 protein expression in tumor stage Ⅲ-Ⅳ ccRCC cancer tissues were significantly higher than the stageⅠ-Ⅱ, the differences were statistically significant (all P ＜ 0.05). There was a significant negative correlation between miR-4313 and TBX15 mRNA expression in cancer tissues (r = -0.671, P = 0.001). Conclusion The expression of miR-4313 in ccRCC cancer tissues decreases，while the expression of TBX15 increases. Both expressions are related to tumor stage and may become new ccRCC tumor markers.

Key words： Clear cell renal cell carcinoma microRNA-4313 T-box transcription factor 15 Clinical significance

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	SU Ji WANG Gang FENG Shiwei

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SU Ji WANG Gang FENG Shiwei. Expression and clinical significance of miR-4313 and TBX15 in clear cell renal cell carcinoma[J]. 中国医药导报, 2021, 18(10): 19-22.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I10/19

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