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| Study on the material basis and related mechanism of acute stroke based on proteomics |
| LIU Tao1 TANG Tao2 |
1.Postdoctoral Workstation, Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Uygur Autonomous Region, Urumqi 830000, China;
2.Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital of Central South University, Hu’nan Province, Changsha 830054, China |
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Abstract Objective To observe the common and differentially expressed proteins (DEPs) in the brain tissue of rats with acute stroke by using quantitative proteomics, and to explore the common material basis and mechanism of acute stroke. Methods Forty male SD rats aged from six to seven weeks were divided into ischemic stroke (IS) sham operation (Sham1) group, IS group, intracranial hemorrhage (ICH) sham operation (Sham2) group and ICH group according to random number table method, with 10 rats in each group. IS rat model of ischemia-reperfusion and ICH model induced by collagenase were established by suture method. After 24 hours in IS group and Sham1 group, after 48 hours in ICH group and Sham2 group, brain tissues were taken for quantitative proteomics analysis after normal saline perfusion. DEPs were identified and analyzed by bioinformatics. The related DEPs were verified by Western blot. Results Three hundred and forty-five and 425 DEPs associated with IS and ICH were screened out, among which 57 were common (20 were up-regulated and 37 were down regulated). GO analysis: exosomes, cytoplasm, plasma membrane and mitochondria were accounted for a high proportion in the classification of cell composition, exosomes, myelin sheath and synapses were ranked the top three in enrichment analysis. Protein binding, same protein binding, domain protein specific binding and phosphatidylserine binding were accounted for a high proportion in molecular functional classification, protein binding, phosphatidylserine binding and myelin sheath structural components were ranked the top three in enrichment analysis. Drug response, hypoxia response and lipopolysaccharide response were accounted for a high proportion in the classification of biological processes, neurofilament assembly, acrylamide reaction and intermediate fiber assembly were ranked in the top three in enrichment analysis. KEGG analysis showed that a total of 17 signaling pathways were abundant, of which amyotrophic lateral sclerosis, pyruvate metabolism, tyrosine metabolism and pyruvate metabolism were the main signaling pathways of acute stroke. Expressions of Nefl, Gap43, Atp6v1f, Th and Atp6v1a in IS group and Sham1 group were compared, expressions of Nefl, Gap43, Atp6v1f, Th and Atp6v1a in ICH group and Sham2 group were compared, the differences were statistically significant (P < 0.05). Nefl was up regulated, while Gap43, Atp6v1f, Th and Atp6v1a were down regulated. Conclusion There are common DEPs in acute stroke, and the core mechanism may be myelin sheath injury and metabolic disorder.
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