热性惊厥患儿外周血泛素羧基末端水解酶L1、神经烯醇化酶表达水平及临床意义

Abstract
Figure/Table
References (28)
Related Citation (15)

Download: PDF (973 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the expression levels of ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1) and neuron-specific enolase (NSE) in peripheral blood of children with febrile convulsion. Methods Retrospective analysis was performed on 97 children with febrile convulsion admitted to the Second People’s Hospital of Wuhu, Anhui Province (“our hospital” for short) from January 2017 to June 2019 as case group. They were divided into simple febrile convulsion group (simple group, 55 cases) and complex febrile convulsion group (complex group, 42 cases) according to disease type; according to the prognosis, the patients were divided into good prognosis group (76 cases) and poor prognosis group (21 cases). A total of 60 children with high fever diagnosed as acute upper respiratory tract infection were selected as control group. Clinical data of each group was compared, the correlation between serum UCH-L1 and NSE levels in children with febrile convulsion and clinical data was analyzed, and the diagnostic value of serum UCH-L1, NSE and adverse prognosis in febrile convulsion were evaluated. Results The levels of blood glucose, lactic acid, UCH-L1 and NSE in case group were higher than those in control group, and the level of hemoglobin in case group was lower than that in control group (all P < 0.01). The levels of blood glucose, lactic acid, UCH-L1 and NSE in complex group were higher than those in simple group, and the level of hemoglobin in complex group was lower than that in simple group (all P < 0.01). The levels of blood glucose, lactic acid, UCH-L1 and NSE in poor prognosis group were higher than those in good prognosis group, and the level of hemoglobin was lower than that in the good prognosis group (all P < 0.01). The levels of UCH-L1 and NSE in children with febrile convulsion were positively correlated with frequency of seizures, duration of seizures, blood glucose and lactic acid (r > 0, P < 0.05), and negatively correlated with hemoglobin (r < 0, P < 0.05). The cut-off value of UCH-L1 in the diagnosis of febrile convulsion was 2.23 ng/mL, area under the curve (AUC) was 0.878 (95%CI: 0.839-0.917), the sensitivity and specificity were 0.83 and 0.87, respectively. The cut-off value of NSE in the diagnosis of febrile convulsion was 9.85 ng/mL, AUC was 0.838 (95%CI: 0.792-0.885), the sensitivity and specificity were 0.77 and 0.83, respectively. The AUC of UCH-L1 combined with NSE in the diagnosis of febrile convection was 0.915 (95%CI: 0.886-0.944), and the sensitivity and specificity were 0.86 and 0.92, respectively. The cut-off value of UCH-L1 for predicting adverse prognosis in children with febrile convulsion was 3.65 ng/mL, AUC was 0.818 (95%CI: 0.768-0.853), the sensitivity and specificity were 0.77 and 0.86, respectively. The cut-off value of NSE for predicting adverse prognosis in children with febrile convulsion was 18.79 ng/mL, AUC was 0.798 (95%CI: 0.760-0.834), the sensitivity and specificity were 0.75 and 0.82, respectively. The AUC of UCH-L1 combined with NSE for predicting poor prognosis in children with febrile convulsion was 0.931 (95%CI: 0.889-0.964), and the sensitivity and specificity were 0.83 and 0.89, respectively. Conclusion The up-regulated expression of UCH-L1 and NSE in serum is involved in the pathogenesis of children with febrile convulsion, and is closely related to the degree of brain injury. Early combined detection can be used as an important indicator for diagnosis of febrile convulsion and prediction of prognosis.

Key words： Febrile convulsion Ubiquitin carboxy-terminal hydrolase-L1 Neuron-specific enolase Diagnosis Prognosis

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LHI Chijia WANG Hongqun

Cite this article:

LHI Chijia WANG Hongqun. Expression levels of ubiquitin carboxy-terminal hydrolase-L1 and neuron-specific enolase in peripheral blood of children with febrile convulsion and their clinical significance[J]. 中国医药导报, 2021, 18(8): 82-86,101.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I8/82

[1] Kimura M，Taketani T，Kurozawa Y. High incidence of status epilepticus and ongoing seizures on arrival to the hospital due to high prevalence of febrile seizures in Izumo，Japan：A questionnaire-based study [J]. Brain Dev，2019， 41（10）：848-853.
[2] 谭丽芬，王明义，郭小芳.小儿热性惊厥的临床特征和脑电图变化分析[J].当代医学，2018，24（13）：4-6.
[3] 朱慧芝，张苗苗，张玉玲.热性惊厥研究进展[J].中国医师进修杂志，2018，41（8）：764-768.
[4] 杨宇，邹新英，郝金斗，等.小儿热性惊厥的临床特点及治疗后血钠和二磷酸甘油酸水平的变化分析[J].黑龙江医学，2017，41（7）：647-648.
[5] Li Y，Sun Y，Li J，et al. Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [J]. J Neurol Sci，2015，357（1/2）：215-221.
[6] 瞿色华，单连强，董淮富，等.血清UCH-L1蛋白在新生大鼠缺氧缺血性脑病评估中的价值[J].蚌埠医学院学报，2017，42（11）：1433-1436.
[7] 陈红梅，陈锐.神经元特异性烯醇化酶在热性惊厥患儿的变化及其临床意义[J].中国实用医药，2016，11（27）：45-46.
[8] 中华医学会儿科学分会神经学组.热性惊厥诊断治疗与管理专家共识（2017实用版）[J].中华实用儿科临床杂志，2017，32（18）：1379-1382.
[9] 胡亚美，江载芳.诸福棠实用儿科学下册[M].北京：人民卫生出版社，2008：1856.
[10] 叶丽娟，黄国强.热性惊厥儿童血钠与神经元特异性烯醇化酶和脑利钠肽水平的相关性研究[J].内蒙古医学杂志，2019，51（2）：174-176.
[11] 杨智全，王海青，支更花，等.外周血UCH-L1、NSE表达水平对热性惊厥患儿脑损伤及预后的评估价值[J].临床和实验医学杂志，2020，19（2）：162-164.
[12] 邓琴芹，廖超平，黄春菊，等.热性惊厥患儿血清hs-CRP、H-FABP与心肌损伤的关系[J].中国妇幼健康研究，2020，31（7）：942-946.
[13] 杨薇斯.小儿热性惊厥的复发因素调查研究[J].中西医结合心血管病电子杂志，2020，8（15）：100-101.
[14] 陈冬梅.小儿热性惊厥复发高危因素的Logistic回归分析[J].中外医学研究，2020，18（14）：22-23.
[15] 张玉军，栾金玲，顾建月，等.热性惊厥患儿血清神经元特异性烯醇化酶变化的临床价值分析[J].浙江临床医学，2016，18（2）：281-282.
[16] Bishop P，Rocca D，Henley JM. Ubiquitin C-terminal hydrolase L1 （UCH-L1）：structure，distribution and roles in brain function and dysfunction [J]. Biochem J，2016，473（16）：2453-2462.
[17] 王光明，成毅，梁巧玲，等.颅脑外伤患者UCH-L1和NSE含量及手术前后变化的临床意义[J].中国医学创新，2019，16（4）：1-6.
[18] 裘艳梅，侯洪涛，叶秀春，等.pH值、乳酸、神经烯醇化酶及泛素羧基末端水解酶L1在早产儿脑损伤中的意义[J].广东医学，2017，38（6）：885-887.
[19] 李兵，胡世颉，闫志强，等.亚低温联合尼莫地平治疗重型颅脑损伤的疗效及对脑血流指标、血清GFAP、UCH-L1水平的影响[J].中西医结合心脑血管病杂志，2017，15（20）：2621-2624.
[20] Pang L，Wu Y，Dong N，et al. Elevated serum ubiquitin C-terminal hydrolase-L1 levels in patients with carbon monoxide poisoning [J]. Clin Biochem，2014，47（1/2）：72-76.
[21] Clifford-Mobley O，Palmer F，Rooney K，et al. Serum neuron-specific enolase measurement for neuro-prognostication post out-of-hospital cardiac arrest：Determination of the optimum testing strategy in routine clinical use [J]. Ann Clin Biochem，2020，57（1）：69-76.
[22] 朱金艳.不同惊厥患儿血清神经元特异性烯醇化酶水平分析[J].中国实用医药，2019，14（23）：71-72.
[23] 杨海玲，崔益秋，管义祥.MRI联合S100B、NSE对不同分期脑出血患者诊断及临床意义[J].中国急救复苏与灾害医学杂志，2020，15（6）：694-697.
[24] 叶远玲，徐伟，汪潞.神经元特异性烯醇化酶联合血乳酸对心肺复苏患者的预测价值[J].中国现代医生，2019， 57（19）：27-30，封3.
[25] 吉翔，华芸，张学军.癫痫发作患儿血清中IGF-1和NSE联合检测的临床意义[J].实用医学杂志，2016，32（4）：650-652.
[26] 宋文桃，乌日莎娜，冯新平，等.动态检测红细胞RDW-CV和血清NSE、EPO在新生儿缺氧缺血性脑病的临床意义[J].内蒙古医科大学学报，2016，38（1）：50-53.
[27] 郝改领，张中馥，马建平，等.热性惊厥患儿外周血NF-κB、NSE的表达及相关因素分析[J].河北医科大学学报，2019，40（6）：662-666.
[28] 展晓玲，廖翠芳，何凯.小儿单纯性与复杂性热性惊厥临床特点及脑电图分析[J].中国医药科学，2019，9（10）：67-69.