EMX1、miR-124a-3、NKX6-1甲基化与胃癌癌前疾病的关系

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Abstract Objective To investigate the correlation between EMX1, miR-124a-3, NKX6-1 methylation and gastric cancer precancerous diseases. Methods From June 2015 to February 2020, 192 patients who underwent gastroscopy with pathological examination at Endoscopy Center of Linyi People’s Hospital of Shandong Province were selected as study objects. They were divided into chronic non-atrophic gastritis group (95 cases), chronic atrophic gastritis group (86 cases), and gastric cancer group (11 cases) according to the development model of gastric cancer (Correa cascade); according to different degrees of chronic gastritis atrophy (pathology), they were divided into low atrophy group (104 cases) and high atrophy group (77 cases); according to the degree of intestinal metaplasia of chronic atrophic gastritis, they were divided into low intestinal metaplasia group (31 cases) and high intestinal metaplasia group (55 cases); according to the Kimura-Takemoto classification of chronic atrophic gastritis under endoscopy, They were divided into low atrophy group (24 cases), moderate atrophy group (48 cases), and high atrophy group (14 cases). The methylation levels at different sites of EMX1, miR-124A-3 and NKX6-1 genes in each group were compared. Results The methylation levels of EMX1 gene (72920455, 72920466 and 72920469) and miR-124a-3 gene (63178433, 63178444 and 63178452) sites in chronic atrophic gastritis group were higher than those in chronic non-atrophic gastritis group, and the differences were all statistically significant (all P < 0.05). The methylation levels of NKX6-1 gene (84496988, 84496933, 84496882, 84496840) sites in gastric cancer group were lower than those in chronic non-atrophic gastritis group and chronic atrophic gastritis group; the methylation levels of NKX6-1 gene (84496988, 84496933 and 84496882, 84496840) sites in chronic atrophic gastritis group were higher than those in chronic non-atrophic gastritis group, and the differences were all statistically significant (all P < 0.05). The methylation levels of EMX1 gene (72920466, 72920469 and 72920484), miR-124a-3 gene (63178433, 63178452 and 63178485) and NKX6-1 gene (84497016, 84496979, 84496975) sites in high atrophy group were higher than those in low atrophy group, and the differences were all statistically significant (all P < 0.05). The methylation levels of EMX1 gene (72920455, 72920466 and 72920469), miR-124a-3 gene (63178334, 63178377 and 63178433) and NKX6-1 gene (84497016, 84496979 and 84496975) sites in high intestinal metaplasia group were higher than those in low intestinal metaplasia group, and the differences were all statistically significant (all P < 0.05). The methylation levels of EMX1 gene (72920581, 72920627 and 72920639) sites in high atrophy group were higher than those in low atrophy group and moderate atrophy group, and the differences were all statistically significant (all P < 0.05); the methylation levels of miR-124a-3 gene (63178345, 63178383 and 63178444) and NKX6-1 gene (84496965, 84496893 and 84496884) sites in high atrophy group were higher than those in low atrophy group, and the differences were all statistically significant (all P < 0.05). Conclusion Methylation of EMX1, miR-124a-3 and NKX6-1 genes may play an important role in the development of precancerous atrophy and intestinal metaplasia of gastric cancer. However, its specific mechanism remains to be further explored.

Key words： DNA methylation Gastric cancer Chronic atrophic gastritis Intestinal metaplasia

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	SUN Liangxu1
	2 PENG Lijun2▲ GUO Anbing2 ZHANG Yanqing2 LU Lin2 JI Buxi2

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SUN Liangxu1,2 PENG Lijun2▲ GUO Anbing2 ZHANG Yanqing2 LU Lin2 JI Buxi2. Correlation between EMX1, miR-124a-3, NKX6-1 methylation and gastric cancer precancerous diseases[J]. 中国医药导报, 2021, 18(5): 18-22.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I5/18

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