厄贝沙坦对自发性高血压大鼠的降压作用及肾脏RAS基因表达分析

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Abstract Objective To explore the effect of Irbesartan on blood pressure of spontaneously hypertensive rats (SHR) and the influence on renal Renin-angiotensin system(RAS) related gene expression. Methods The SHR rats were randomly divided into model group (15 mg/[kg·d], drinking water), Irbesartan group (15 mg/[kg·d], Irbesartan), six rats in each group; another six Wistar rats were served as normal control group (15 mg/[kg·d], drinking water) and each group was administered for eight weeks. The blood pressure changes of rats at three hours and 24 h after administration of each week were measured by the non-invasive blood pressure meter; RT-qPCR was used to detect the expression of angiotensin converting enzyme 2 (ACE2), angiotensin Ⅱ receptor-1 (AT1R) and AT2R mRNA in kidney tissue. Results The systolic blood pressure (SBP) of the Irbesartan group and model group at three hours and 24 h after administration showed highly statistically significant difference (P ＜ 0.01). Among them, SBP in the Irbesartan group was lower than that in the model group at three hours and 24 h after administration at the first time, two, four, six and eight weeks, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The difference in diastolic blood pressure (DBP) between the Irbesartan group and model group at three hours and 24 h after administration was highly statistically significant (P ＜ 0.01). Among them, the DBP of the Irbesartan group was lower than that of the model group three hours after administration at the first time, while were lower than those of the model group at three hours and 24 h after administration at two, four, six, and eight, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Compared with the model group, the relative expression of ACE2 mRNA in the Irbesartan group was decreased, while the relative expression of AT2R mRNA was increased, and the differences were statistically significant (P ＜ 0.05). Conclusion Irbesartan has a significant effect on lowering blood pressure. After Irbesartan reduces blood pressure, the body produces a protective effect of blood pressure regulation, so that the expression of ACE2 is reduced. Moreover, AT1R and AT2R have a functional interaction in blood pressure regulation. Blocking AT1R reduces blood pressure, and may lead to the stimulating and elevating effect of opposite AT2R.

Key words： Irbesartan Spontaneously hypertensive rats Angiotensin converting enzyme 2 Angiotensin Ⅱ receptor-1 Angiotensin Ⅱ receptor 2

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	CHEN Sisi1 XUE Xiaomin2 LI Yu2 ZHU Bing’er3 CHEN Lihao3 WU Yue3 WU Renzhao1

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CHEN Sisi1 XUE Xiaomin2 LI Yu2 ZHU Bing’er3 CHEN Lihao3 WU Yue3 WU Renzhao1. Effect of Irbesartan on blood pressure of spontaneously hypertensive rats and analysis of renal RAS gene expression[J]. 中国医药导报, 2021, 18(5): 10-13.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I5/10

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