酸枣仁-五味子药对配比筛选及半仿生提取工艺研究

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Abstract Objective To screen the optimal ratio of Jujube Kernel-Schisandra rnedcine pair on sedative and hypnotic effect, and the semi-bionic extraction technology of the ratio of the drug pair was optimized. Methods Fifty mice were randomly divided into blank group, positive drug group, Jujube Kernel-Schisandra (15 g∶6 g group, 12 g∶6 g group and 6 g∶6 g group), each group was 10 mice. The blank group was given distilled water and the positive drug group was given 0.13% Estazol Lun water solution, while the drug administration group was given suspension with corresponding ratio. Mice were induced to sleep by intraperitoneal injection of different doses of Sodium Pentobarbital, and the sleep status, sleep latency and sleep duration of mice in each group were recorded. The content changes of γ-aminobutyric acid (GABA), glutamate(Glu), glutamine(Gln), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in brain tissue fluid of mice in each group were determined to screen the optimal ratio; the comprehensive scores of GABA and 5-HT were uesd as the response values, the best technology for screening semi-bionic extraction by Box-Behnken response surface method. Results Compared with blank group, sleep latency and sleep duration of mice in positive drug group, 15 g:6 g group and 6 g:6 g group were significantly shortened and prolonged (P < 0.05 or P < 0.01). Compared with positive drug group, sleep latency of 12 g:6 g group was significantly prolonged (P < 0.05); Compared with 15 g:6 g group, sleep latency in 12 g:6 g group was significantly prolonged (P < 0.05). Compared with blank group, the contents of GABA in brain of mice in positive drug group and 15 g:6 g group were increased and Glu and Gln in positive drug group and 15 g:6 g group were decreased (P < 0.05 or P < 0.01). Compared with positive drug group, the contents of GABA in 12 g:6 g group and 6 g:6 g group were decreased, while Glu and Gln contents were increased (P < 0.05). Compared with 15 g:6 g group, the contents of GABA in 12 g:6 g group were decreased, while the contents of Gln in 6 g:6 g group were increased (P < 0.05). Compared with blank group, the contents of 5-HT in positive drug group, 15 g:6 g group, 12 g:6 g group and 6 g:6 g group were increased (P < 0.05 or P < 0.01). Compared with positive drug group, the contents of 5-HT in 12 g:6 g group were decreased (P < 0.05). Compared with 15 g:6 g group, the contents of 5-HT in 12 g:6 g group were decreased (P < 0.05). Compared with blank group, the contents of 5-HIAA in positive drug group were decreased (P < 0.05). Compared with positive drug group, the contents of 5-HIAA in 15 g:6 g group, 12 g:6 g group and 6 g:6 g group were increased (P < 0.05 or P < 0.01). The optimal technology of semi-bionic extraction was that the amount of artificial gastric juice was 9.5 times and the time was 2.5 h for the first time. The dosage of artificial intestinal fluid was 6.0 times and the time was 3.6 h for the second time. Conclusion The optimum ratio of 15 g:6 g (corresponding to the daily dose of human body) for sedative and hypnotic effect of Jujube Kernel-Schisandra is obtained. The semi-bionic extraction technology is reliable and operable under the optimal ratio condition.

Key words： Jujube Kernel-Schisandra Different ratio Box-Behnken response surface method Semi-bionic extraction

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	RAN Jianxin1 HU Denghui2 WANG Xiaojie3

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RAN Jianxin1 HU Denghui2 WANG Xiaojie3. Study on screening medicine pair ratio and semi-bionic extraction technology of Jujube Kernel-Schisandra[J]. 中国医药导报, 2021, 18(4): 18-23.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2021/V18/I4/18

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