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| Effects of Selenium-enriched Danggui Yinyanghuo Recipe on SOD, MDA and serum sex hormone in rat testis |
| LI Jintao1 LI Shijun2 SONG Zhangxin2 XIANG Kui2 ZHAO Hua2 CUI Yingdong2 TIAN Zhaohui3 |
1.Department of Medicine, Hubei Minzu University, Hubei Province, Enshi 445000, China;
2.Department of Urology and Andrology, the National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, Enshi 445000, China;
3.Department of Medical Administration, the National Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, Enshi 445000, China |
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Abstract Objective To study the effects of different doses of Selenium-enriched Danggui Yinyanghuo Recipe on superoxide dismutase (SOD), malondialdehyde (MDA) and serum sex hormones (follicle stimulating hormone [FSH], luteinizing hormone [LH] and testosterone [T]) in rats testis. Methods Ten male SD rats were randomly selected as the normal group (gavage physiological saline), and the remaining rats were divided into four groups according to random number table method: model control group (gavage adenine), Selenium-enriched Danggui Yinyanghuo Recipe high dose group (gavage adenine + 15 days later, gavage 20.92 g/[kg·d] selenium-enriched traditional Chinese medicine), middle dose group (gavage adenine + 15 days later, gavage 10.46 g/[kg·d] selenium-enriched traditional Chinese medicine), low dose group (gavage adenine + 15 days later, gavage 5.23 g/[kg·d] selenium-enriched traditional Chinese medicine). The experiment lasted for 45 days with 12 rats in each group. The general state, SOD and MDA of testis, serum sex hormone and testicular morphology of rats were detected and compared in each group. Results Observation of general state: from the fourth day of modeling, the model control group and the Selenium-enriched Danggui Yinyanghuo Recipe groups showed clinical manifestations of reduced diet, decreased body weight and increased urine volume. After the implementation of drug intervention, the diet and body weight of the low, medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe gradually increased, and the urine volume and other clinical manifestations gradually tended to be normal. Compared with normal group, SOD decreased and MDA increased in testicular homogenate of model control group, with high statistical significance (all P < 0.01). Compared with the model control group, SOD in the Selenium-enriched Danggui Yinyanghuo Recipe high dose and medium dose groups increased to varying degrees, with high statistical significance (all P < 0.01). However, there was no significant difference between Selenium-enriched Danggui Yinyanghuo Recipe low dose group and model control group (P ＞ 0.05). Compared with the model control group, MDA in the high, medium and low dose groups of Selenium-enriched Danggui Yinyanghuo Recipe was decreased to different degrees, with high statistical significance (all P ＜ 0.01). Compared with normal group, serum FSH and LH were increased in model control group, while serum T was decreased, with high statistical significance (all P ＜ 0.01). Compared with model control group, serum FSH and LH were decreased and serum T was increased in the high and medium dose groups of Selenium-enriched Danggui Yinyanghuo Recipe, with high statistical significance (all P ＜ 0.01). Histopathology of testis showed obvious testicular injury in model control group, and improvement in low, medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe. Conclusion Selenium-enriched Danggui Yinyanghuo Recipe can improve the levels of SOD, MDA and serum sex hormones in the testis of rats after adenine modeling, and has a certain therapeutic effect on the testis injury caused by adenine.
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