P16蛋白表达在分流宫颈上皮内瘤变Ⅱ中的临床意义

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Abstract Objective To observe the expression of p16 protein in the tissues of patients with different cervical lesions, and to investigate the feasibility of p16 protein as a reference index of CIN Ⅱ. Methods From January 2013 to August 2015, 170 cases underwent colposcopy and biopsy in Zhongshan BOAI Hospital Affiliated to Southern Medical University proved cervicitis, CIN Ⅰ and CIN Ⅱ, CIN Ⅲ and four cervical carcinoma group were selected, the expression of p16 were observed in different cervical lesions by p16 immunohistochemical staining, efficiency of the test of p16 protein in the diagnosis of CIN Ⅱ and above statistic (CINⅡ+, including CIN Ⅱ, CIN Ⅲ and cervical cancer) performance. And 170 cases of CIN Ⅱ patients were followed up every six months until 2 years after diagnosis with abnormal colposcopic biopsy, the relationship between the expression of p16 and outcome measures of CIN Ⅱ patients lesions was analyzed. Results ①The p16 positive rates of the cervicitis group and CIN Ⅰ group and CIN Ⅱ group, CIN Ⅲ and cervical cancer groups were 3.53%, 41.18%, 81.18%, 100% respectively, the difference was statistically significant (P < 0.05); the positive rate of p16 protein in the CINⅡ group was significantly higher than that of cervicitis group and CIN Ⅰ group, the differences were statistically significant (P < 0.01), the positive rate of p16 protein in the CIN Ⅲ group and cervical cancer group were significantly higher than CINⅡ group, CINⅠgroup, cervicitis group, the differences were statistically significant (P < 0.01). ②The sensitivity, specificity, coincidence rate, positive predictive value and negative predictive value of p16 protein in the detection of CINⅡ+ were respectively 90.59%, 77.65%, 84.12%, 80.21% respectively, 89.19%. ③62 cases (44.93%) in 138 CIN Ⅱ patients with positive expression of p16 protein had abnormal follow-up, 3 cases (9.38%) of 32 cases of disease progression CIN Ⅱ patients with negative expression of p16 protein had abnormal follow-up, the difference of two were statistically significant (P < 0.05). Conclusion The positive rate of p16 protein increased with the severity of cervical lesions. Detection of p16 protein shows better sensitivity, specificity, coincidence rate, positive predictive value and negative predictive value in the detection of CINⅡ+. The expression of p16 protein can predict the progression of CIN Ⅱ disease, and can be applied to the shunt of CIN Ⅱ in clinic.

Key words： Cervical intraepithelial neoplasia Cyclin-dependent kinase inhibitor p16 ;Cervical cancer High-grade squamous intraepithelial lesion, HSIL Low-grade squamous intraepithelial lesion

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	HUANG Liping LIN Xia▲

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HUANG Liping LIN Xia▲. Clinical significance of p16 protein expression in cervical intraepithelial neoplasia Ⅱ[J]. 中国医药导报, 2018, 15(2): 83-86,91.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I2/83

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